In-Vitro Anticancer and Free Radical Scavenging Potential of Compound Formulation Used in Unani System of Medicine

Mohd Nazir Mannan; Munawwar Husain Kazmi; Alokananda Chakraborty; Mohammad Zakir; Tasleem Ahmad; K. Lahari

doi:http://dx.doi.org/10.5667/CellMed.2020.0027

CELLMED

Abbr : CellMed
2020, 10(4), pp.27-27
DOI :10.5667/CellMed.2020.0027
Publisher : Cellmed Orthocellular Medicine and Pharmaceutical Association
Research Area : General Medicine

Mohd Nazir Mannan ¹ , Munawwar Husain Kazmi ² , Alokananda Chakraborty ³ , Mohammad Zakir ⁴ , Tasleem Ahmad ⁵ , K. Lahari ⁶

¹ Department of Ilmul Advia (Pharmacology), Markaz Unani Medical College & Hospital
² Department of Ilmul Advia, National Research Institute of Unani Medicine for Skin Disorders (NRIUMSD)
³ National Research Institute of Unani Medicine for Skin Disorders (NRIUMSD)
⁴ Department of Ilmul Advia (Pharmacology), National Research Institute of Unani Medicine for Skin Disorders (NRIUMSD)
⁵ National Research Institute of Unani Medicine for Skin Disorders (NRIUMSD)
⁶ National Research Institute of Unani Medicine for Skin Disorders (NRIUMSD)

ABSTRACT

Cancer is one of the leading cause of mortality in India as well as worldwide. The management of cancer by conventional therapy has shown life threatening adverse effects. The researchers are now exploring the natural way of treatment. Unani system of medicine have rich literature for cancer and many compound formulations have been described in this system. Unani system of medicine is based on holistic approach and treat human being as a unit with natural herbs, mineral and animal origin drugs. An important compound Unani formulation (CUF) from the literature has been chosen to explore the Unani claim of its anticancer activity. The phytochemical constituents were assessed using standard phytochemical screening method. Antioxidant property of this formulation was assessed by DPPH assay. The DPPH free radical scavenging assay was carried out by colorimetric method and ascorbic acid was taken as a positive control. Three different extracts of CUF on different concentrations were used to screening on human breast cancer (BCC) MCF-7 cell line. For the estimation of in-vitro cytotoxic potency of the investigated extracts was assessed on MTT assay by using trypan blue method and paclitaxel was used as the standard. Hydro-ethanolic (HE) extract showed highest free radical scavenging activity among all extracts. DPPH Assay showed substantial antioxidant activity of these extracts in hydro-ethanol extract at 1µg concentration of CUF. The CUF showed antioxidant and anticancer activity. The claim made by Unani physician has been proved.
KEYWORDS

Anti-cancer Activity , Antioxidant , Breast cancer cell line , MCF-7 , Saratan , DPPH Assay , MTT Assay
REFERENCES (52)

[journal] Adeyemi, TOA / 2014 / Phytochemical Screening and Health Potentials of Morinda Lucida Benth / International Journal of Innovation and Scientific Research 11 (2) : 515~519

[journal] Ahuja R / 2013 / Evaluation of Anticancer Potential of Terminalia Chebula Fruits against Ehrlich Ascites Carcinoma Induced Cancer in Mice / Journal of Scientific and Innovative Research 2 (3) : 549~554

[book] Al-Qamri MH / 2008 / Ghina Mana (Urdu Translation by CCRUM) / CCRUM, Dept. of AYUSH, Ministry of Health and Family Welfare : 508~510

[web] Anonymous / 2019 / National Cancer Institute / https://www.cancer.gov/about-cancer/understanding/statistics

[journal] Alam MA / 2017 / Unani Approach to Cancer (Sartan) and Its Management / International Journal of Unani and Integrative Medicine 1 (2) : 01~03

[journal] Aynehchi Y / 1982 / Survey of Iranian Plants for Saponins, Alkaloids, Flavonoids and Tannins. II / International Journal of Crude Drug Research 20 (2) : 61~70

[journal] Baskar R / 2012 / Cancer and Radiation Therapy: Current Advances and Future Directions / International Journal of Medical Sciences 9 (3) : 193~199

[book] Bernard W Stewart / 2003 / World Health Organization (WHO: OMS): International Agency for Research on Cancer: World Cancer Report / IARC Press : 182~186

[journal] Chakraborti SK / 1974 / Variations in the antitumour constituents ofWithania somnifera dunal / Experientia 30 : 852~853

[journal] Chatterjee D / 2011 / Evaluation of Antitumor Activity of Cuscuta ReflexaRoxb(Cuscutaceae)Against Ehrlich Ascites Carcinoma in Swiss Albino Mice / Tropical Journal of Pharmaceutical Research 10 (4) : 447~454

[web] Davis CP / Cancer / https://www.medicinenet.com/cancer/article.htm

[journal] Diamandopoulos GT / 1996 / Cancer: An Historical Perspective / Anticancer Res 16 (4A) : 1595~1602

[journal] FarooqU / 2014 / A Comparative Study of Phytochemical Investigation of Xanthium Strumarium Medicinal Plant / IJRPC 4 (1) : 96~100

[journal] Fridovich I. / 1998 / Oxygen Toxicity: A Radical Explanation / The Journal of Experimental Biology 201 (Pt 8) : 1203~1209

[journal] Gören N / 2002 / Chemical characterization and biological activities of the genus Tanacetum (Compositae) / Bioactive Natural Products (Part H) Studies in Natural Products Chemistry 27 (H) : 547~658

[journal] GulcinW / 2004 / Comparison of Antioxidant Activity of Clove (Eugenia Caryophylata Thunb) Buds and Lavender (Lavandula Stoechas L.) / Food Chemistry 87 (3) : 393~400

[confproc] Hamuel J / 2012 / Phytochemicals: Extraction Methods, Basic Structures and Mode of Action as Potential Chemotherapeutic Agents / Phytochemicals -A Global Perspective of Their Role in Nutrition and Health : 2~4

[book] Ibn-e-Rushd / 1987 / Kitab-Ul-Kulliyat / CCRUM, Dept. of AYUSH, Ministry of Health andFamily Welfare : 81~

[book] Ibne-al-Qif-al-Maseehi / 2000 / Kitab-Al-Umda-Fil-Jarahat / Dept. of AYUSH, Ministry of Health and Family Welfare : 169~170

[journal] Ignacimuthu S / 2006 / Ethnobotanical investigations among tribes in Madurai District of Tamil Nadu (India) / J Ethnobiology Ethnomedicine 2 (1) : 25~

[journal] Jeena KJ / 1999 / Effect of Emblica Officinalis, Phyllanthus Amarus and Picrorrhiza Kurroa on N-Nitrosodiethylamine Induced Hepatocarcinogenesis / Cancer Letters 136 (1) : 11~16

[journal] Joy KL / 2000 / Effect of Picrorrhiza Kurroa Extract on Transplanted Tumours and Chemical Carcinogenesis in Mice / J Ethnopharmacol 71 : 261~266

[journal] Kaileh M / 2007 / Screening of indigenous Palestinian medicinal plants for potential anti-inflammatory and cytotoxic activity / Journal of Ethnopharmacology 113 (3) : 510~516

[journal] KatiyanSS / 2016 / Co-Delivery of Rapamycin and Piperine Loaded Polymeric Nano-Particles for Breast Cancer Treatment / Drug Delivey : 1~9

[book] Kirtikar KR / 1999 / Indian Medicinal Plants / International Book Distributors : 1017~1020

[book] Kirtikar, KR / 1999 / Indian Medicinal Plants / International Book Distributors : 1741~1742

[book] Kokate CK / 2017 / Pharmacognosy / Nirali Prakashan : 1.1~1.6

[journal] KumarA / 2014 / A Review of Traditional Anticancer Nano-Medicine: Triphala / The Pharma Innovation Journal 3 (7) : 60~66

[journal] Mahmood N / 1997 / Constituents of Cuscuto Reflexa are anti-HIV Agents / Antiviral Chemistry and Chemotherapy 8 (1) : 70~74

[book] Majoosi A. / 2010 / Kamil-Al-Sana’a (Arabic) / CCRUM, Dept. of AYUSH, Ministry of Health and Family Welfare : 240~

[journal] Mannan MN / 2020 / Standardization and Development of Pharmacopoeial Standard Operating Procedures (SOPs)of Classical Unani Formulation / CellMed Orthocellular Medicine Pharmaceutical Association 10 (2) : 1~8

[book] Nadkarni M / 2010 / Materia Medica / Popular Prakashan : 50~51

[journal] Nobori T / 1994 / Deletions of the cyclin-dependent kinase-4 inhibitor gene in multiple human cancers / Nature 368 (6473) : 753~756

[journal] Okunlola A / 2007 / Evaluation of Pharmaceutical and Microbial Qualities of Some Herbal Medicinal Products in South Western Nigeria / Tropical Journal of Pharmaceutical Research 6 (1) : 661~670

[book] Quraeishi AH / 2002 / Cancer Ya Sartan: Ek Ta’ruf. Pandhra Rozah-Tibb-E-Mashriq: Delhi: Cancer Special Issue / New Public Press : 72~

[journal] Rajkumar V / 2011 / Antioxidant and anti-neoplastic activities of Picrorhiza kurroa extracts / Food and chemical toxicology 49 (2) : 363~369

[book] Rampuri NG / 2011 / Khazanul Advia / Idara Kitab-Us-Shifa : 226~227

[book] Razi ABMZ / 2002 / Kitab-Al-Hawi / CCRUM, Dept. of AYUSH, Ministry of Health and Family Welfare : 9~24

[journal] Sudhakar A / 2009 / History of Cancer, Ancient and Modern Treatment Methods / J Cancer Sci Ther 1 (2) : 1~4

[book] Samarqandi AN / 2007 / Sharah AsbaabTarjumah Kabeer / EjajPublishing House : 21~

[book] Samarqandi AN / 2009 / Sharah Asbab / Idara Kitab-al-Shifa : 177~186

[book] Sofowora A / 1993 / Medicinal Plants and Traditional Medicine in Africa / Spectrum Books Ltd., Sunshine House : 191~289

[journal] Sunyana Jain / 2009 / Ayurvedic Medicines in the Treatment of Cancer / Journalof Chinese Integrative Medicine 7 (11) : 1096~1099

[book] Tabri M / 1997 / Al-Maolijat-e-Buqratiya / CCRUM, Dept. of AYUSH, Ministry of Health and Family Welfare : 244~248

[journal] Udavant PB / 2012 / Preliminary screening of Cuscuta reflexastems for Anti-inflammatory and cytotoxic activity / Asian Pacific Journal of Tropical Biomedicine : S1303~S1307

[journal] Urruticoechea A / 2010 / Recent Advances in Cancer Therapy: An Overview / Current Pharmaceutical Design 16 (1) : 3~10

[journal] Vaghasiya Y / 2011 / Phytochemical Analysis of Some Medicinal Plants from Western Region of India / Research Journal of Medicinal Plant 5 : 567~576

[web] Vorobiof DA / 2007 / The Cancer Burden in Africa / https://www.researchgate.net/publication/5845578_The_cancer_burden_in_Africa

[journal] Weiss L / 2000 / Early Concepts of Cancer / Cancer Metast Rev 19 (3-4) : 215~217

[web] WHO / 2018 / World Health Organisation / Key Facts about Cancer / https://www.who.int/cancer/about/facts/en/

[journal] Yahaya U / 2019 / Antifungal activity of Parkiabiglobosa extract on pathogenic strain of Candida albicans / J. Applied Sci, 19 : 235~240

[journal] Young IS / 2001 / Antioxidant in Health Disease / J Clin Pathol 54 : 176~186
KCI Citation (0)

KJC Korea
Journal Central

Statistics

Tools