The purpose of this study was to examine low interpersonal stress as a mechanism by which calling affects job satisfaction. Specifically, this study examined the mediating effect of interpersonal stress on the relationship between calling and job satisfaction. Also, this study investigated the moderating effect of living a calling on the relationship between interpersonal stress and job satisfaction. Data were collected from 359 secondary school teachers via an online survey. The results showed that calling positively predicts job satisfaction, which was partially mediated by low interpersonal stress. Also, when the level of living a calling was high, the negative effect of interpersonal stress on job satisfaction was weakened, confirming the moderating effect of living a calling. Besides, the mediating effect of interpersonal stress between calling and job satisfaction was moderated by living a calling, suggesting that the moderated mediation effect was significant. Based on these results, implications, limitations, and directions for future research were discussed.