Purpose: The purpose of this study was to find out the difference in the priority level of recognition for universal design application in public spaces by gender. Method: ANOVA(analysis of variance) and post-hoc test were conducted to determine the priority level of recognition and pattern for the disabled, non-disabled, and experts classified into males and females. Results: There was no gender difference in the comparison by sector for all males and females. However, in comparing of domains and facilities, women showed a higher level of recognition than men in the building sector and cross domain. When comparing space consumers and producers by dividing them into male and female groups, women showed a higher level of recognition than men in producers, but there was no gender difference between consumers. In comparison by sector, domain and facility, women producers also showed a higher level of recognition in the road sector, park and recreation sector, sidewalk domain, four-spaces in the park and recreation sector, and six-spaces in the building sector than men producers. Also, in the building sector, women producers and consumers showed a higher recognition level than men. Comparing the disabled, non-disabled people and experts by dividing them into male and female groups, in the case of non-disabled people and experts, women showed a higher level of recognition than men, while men showed a higher level of recognition than women in the disabled. In addition, there were differences in recognition patterns in many spaces and facilities by gender. Implications: This study is meaningful in comparing the differences in the priority level of recognition and patterns between men and women to apply universal design for people of all ages and both sexes.