Comparison of Mineralization in Each Passage of Dental Pulp Stem Cells from Supernumerary Tooth

JISUN SHIN; Kim Jongbin

doi:10.5933/JKAPD.2017.44.3.350

The purpose of this study was to evaluate the difference of differentiation potential in each passage of dental pulp stem cells from supernumerary tooth (sDPSCs). The sDPSCs were obtained from a healthy 6-year-old male patient under the guidelines and got the informed consent. Cells were cultured until passage number 16 and divided into two groups; 1 - 8 passages as a young group and 9 - 16 passages as an old group. It was taken 2.25 ± 0.46 days in a young group and 3.25 ± 0.46 days in an old group to propagate cells of each passage until confluence and there were statistically significant differences between two groups (p < 0.05). In every passage, cell morphology was observed with microscope and evaluated the capacity to form high levels of minerals by alizarin red solution staining after treating differentiation medium. Fibroblast-like, spindle shaped, elongated cells and a few nodules were found in uninduced cultures of passage number 1, 8 and 9. But at 16 passage culture, cell size became larger and broader and observed with more nodules. After inducing differentiation, mineralized nodules were detected at the first passage of 7th day culture whereas at the 8 passage culture, nodules were seen clearly at 14th day culture. In addition, the amount of mineralized nodules were remarkably decreased after passage 9. From the data presented in this study, it is recommended to use sDPSCs of passage number within 8 for utilizing as stem cells.

Citation status

KCI Citation Counts (1)
열림/닫힘 버튼
REFERENCES (36)
열림/닫힘 버튼
* 2021년 이후 발행 논문의 참고문헌은 현재 구축 중입니다.

[journal] Smith A / 2006 / A glossary for stem-cell biology / Nature 441 : 1060~1060

[journal] Evans MJ / 1981 / Establishment in culture of pluripotential cells from mouse embryos / Nature 292 : 154~156

[journal] Almeida-Porada G / 2001 / Adult stem cell plasticity and methods of detection / Rev Clin Exp Hematol 5 : 26~41

[journal] Temple S / 2001 / Stem cell plasticity-building the brain of our dreams / Nature Reviews Neuroscience 2 : 513~520

[journal] Matsubara T / 2004 / A new technique to expand human mesenchymal stem cells using basement membrane extracellular matrix / Biochem Biophys Res Commun 313 : 503~508

[journal] Baxter MA / 2004 / Study of telomere length reveals rapid aging of human marrow stromal cells following in vitro expansion / Stem Cells 22 : 675~682

[journal] Bianco P / 2001 / Bone marrow stromal stem cells: nature, biology, and potential applications / Stem Cells 19 : 180~192

[journal] Bruder SP / 1997 / Growth kinetics, selfrenewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation / J Cell Biochem 64 : 278~294

[journal] Gronthos S / 2000 / Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo / Proc Natl Acad Sci USA 97 : 13625~13630

[journal] Seo BM / 2004 / Investigation of multipotent postnatal stem cells from human periodontal ligament / Lancet 364 : 149~155

[journal] Morsczeck C / 2005 / Isolation of precursor cells (PCs) from human dental follicle of wisdom teeth / Matrix Biol 24 : 155~165

[journal] Sonoyama W / 2006 / Mesenchymal stem cell-mediated functional tooth regeneration in swine / PLoS One 1 : e79~

[journal] Miura M / 2003 / SHED: stem cells from human exfoliated deciduous teeth / Proc Natl Acad Sci U S A 100 : 5807~5812

[journal] Huang WH / 1992 / Mesiodens in the primary dentition stage: a radiographic study / ASDC J Dent Child 59 : 186~189

[journal] Huang AHC / 2008 / Isolation and characterization of dental pulp stem cells from a supernumerary tooth / J Oral Pathol Med 37 : 571~574

[journal] Hayflick L / 1961 / The serial cultivation of human diploid cell strains / Exp Cell Res 25 : 585~621

[journal] Till JE / 1961 / A direct measurement of the radiation sensitivity of normal mouse bone marrow cells / Radiat Res 14 : 213~222

[journal] Wagers AJ / 2004 / Plasticity of adult stem cells / Cell 116 : 639~648

[journal] Phinney DG / 1999 / Donor variation in the growth properties and osteogenic potential of human marrow stromal cells / J Cell Biochem 75 : 424~436

[journal] Ryu E / 2008 / Identification of senescenceassociated genes in human bone marrow mesenchymal stem cells / Biochem Biophys Res Commun 371 : 431~436

[journal] Wall ME / 2007 / Effects of serial passaging on the adipogenic and osteogenic differentiation potential of adipose-derived human mesenchymal stem cells / Tissue Eng 13 : 1291~1298

[journal] Park BW / 2007 / Osteogenic phenotypes and mineralization of cultured human periosteal-derived cells / Arch Oral Biol 52 : 983~989

[journal] Rho GJ / 2009 / Porcine mesenchymal stem cells-current technological status and future perspective / Front Biosci (Landmark Ed) 14 : 3942~3961

[journal] Huang GJ / 2009 / Mesenchymal stem cells derived from dental tissues vs. those from other sources:their biology and role in regenerative medicine / J Dent Res 88 : 792~806

[journal] Graziano A / 2008 / Dental pulp stem cells: a promising tool for bone regeneration / Stem Cell Rev 4 : 21~26

[journal] Stenderup K / 2003 / Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells / Bone 33 : 919~926

[journal] Huang AH / 2008 / Isolation and characterization of dental pulp stem cells from a supernumerary tooth / J Oral Pathol Med 37 : 571~574

[journal] Lindroos B / 2008 / Characterisation of human dental stem cells and buccal mucosa fibroblasts / Biochem Biophys Res Commun 368 : 329~335

[journal] Karaoz E / 2010 / Isolation and in vitro characterisation of dental pulp stem cells from natal teeth / Histochem Cell Biol 133 : 95~112

[journal] Berchem G / 2002 / Cathepsin-D affects multiple tumor progression steps in vivo: proliferation, angiogenesis and apoptosis / Oncogene 21 : 5951~

[journal] Mets T / 1981 / In vitro aging of human bone marrow derived stromal cells / Mech Ageing Dev 16 : 81~89

[journal] Min JH / 2011 / Dentinogenic potential of human adult dental pulp cells during the extended primary culture / Hum Cell 24 : 43~50

[journal] 박세아 / 2009 / Characterization of Umbilical Cord-derived Stem Cells during Expansion in Vitro / Clinical and Experimental Reproductive Medicine / 대한생식의학회 36(1) : 23~34

[journal] Izadpanah R / 2006 / properties of mesenchymal stem cells derived from bone marrow and adipose tissue / J Cell Biochem 99 : 1285~1297

[journal] Conget PA / 1999 / Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells / J Cell Physiol 181 : 67~73

[journal] Deans RJ / 2000 / Mesenchymal stem cells: biology and potential clinical uses / Exp Hematol 28 : 875~884

KJC Korea
Journal Central

Statistics

Tools

Sharing

Issue List

Citation status