1강릉원주대학교 치과대학 소아∙청소년치과학교실 및 구강과학연구소 2강릉원주대학교 3강릉원주대학교 4강릉원주대학교 5강릉원주대학교
The aim of this study was to evaluate the antimicrobial effect of photodynamic therapy (PDT) using plaque disclosing agent, 10 - 20 mM erythrosine, as a photosensitizer.
Multispecies cariogenic biofilms containing Streptococcus mutans , Lactobacillus casei and Candida albicans were formed on hydroxyapatite disc. 20 μM, 10 mM and 20 mM erythrosine were applied as a photosensitizer for 3 minutes, and then light-emitting diode (LED) irradiated for 24 seconds. Colony-forming unit (CFU) were measured and biofilms were observed using confocal laser scanning microscopy (CLSM). CFU were significantly decreased in the PDT groups using 10 - 20 mM erythrosine (10 mM, 20mM) and the results were also confirmed by CLSM.
This study confirms the high antimicrobial effect of photodynamic therapy using plaque disclosing agent as a photosensitizer.