Effects of Prunellae Herba on Proteoglycan Degradation in Collagenase-induced Rat Osteoarthritis Model
Heon-Sik Ryu, O.M.D., Su-Hyeon Jeong, O.M.D., Sun-Jung Kim, O.M.D., Il-Bok Seo*
Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung UniversityDept. of Anatomy, College of Oriental Medicine, Se-Myung University*
This study was to investigate the effects of Prunellae Herba on Proteoglycan(PG) degradation by measuring body weight, Glycosaminoglycan(GAG), Interleukin-1β(IL-1β) in synovial fluid, and PG content of articular cartilage of femur in collagenase-induced arthritis in rats.
Arthritis was induced by injection of collagenase(0.1 ml) into knee joint of rats. Arthritic rats were divided into control(n=8) and treated(n=8) group. Control group was taken normal saline for 15 days and treated group was taken extracts of Prunellae Herba for same duration. Normal group(n=8), non-arthritic group, was injected with normal saline and was taken normal saline for 15 days. Body weight was measured at 0, 10, 15 days after injection. GAG, IL-1β in synovial fluid were measured with ELISA kit at 15 days after injection. PG content of articular cartilage of femur, represented by safranine O staining, was measured at 15 days after injection. Histopathological study on the articular cartilage of knee joint was investigated at 15 days after injection.
Body weight of treated group was not significantly increased, PG of treated group was significantly increased, and GAG was significantly decreased compared with control group. But IL-1β was not significantly decreased.
On the basis of these results, we concluded that Prunellae Herba has inhibiting effects on the Proteoglycan degradation in collagenase-induced rat osteoarthritis model.