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Journal Central

Effects of Neutral Eohyeol(Yuxue) Herbal Acupuncture and Dangkisoo-san(Dangguixu-san) on Fracture Healing in the Early Stage in Rats Hye-Lim Ahn, O.M.D., Mi-Suk Shin, O.M.D., Sun-Jong Kim, O.M.D., Jin-Bong Choi, O.M.D. Dept. of Oriental Rehabilitation Medicine, Dong-Shin University Mok-Dong Oriental HospitalDept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Shin University* Objectives : This study was designed to evaluate the effects of Neutral Eohyeol(Yuxue) herbal acupuncture and Dangkisoo-san(Dangguixu-san) on fracture healing in rats. Methods : We divided the subjects into 5 groups : normal, control with no treatment, Sample Ⅰ taken with Dangkisoo-san(Dangguixu-san), Sample Ⅱ taken with Neutral Eohyeol(Yuxue) herbal acupuncture and sample Ⅲ taken with Neutral Eohyeol(Yuxue) herbal acupuncture and Dangkisoo-san(Dangguixu-san) groups. We induced penetrating fracture on femur in control, sample Ⅰ, Ⅱ and Ⅲ groups. And after serum T-ALP, serum osteocalcin, histological changes, and the degree of TGF-β1 in each group were observed at the 7th day and at the 14th day respectively. Results : All the sample groups showed significant changes in serum T-ALP, histological observation and TGF-beta 1 compared with control group. Especially sample Ⅲ was more effective than other groups in fracture healing. However, serum osteocalcin insignificantly increased at the 14th day. It seemed that 14 day was too short to evaluate osteocalcin. Conclusions : It is suggested that Neutral Eohyeol(Yuxue) herbal acupuncture and Dangkisoo-san(Dangguixu-san) may play a significant role in fracture healing and these two treatments could be more effective when used together.

Effects of Prunellae Herba on Proteoglycan Degradation in Collagenase-induced Rat Osteoarthritis Model Heon-Sik Ryu, O.M.D., Su-Hyeon Jeong, O.M.D., Sun-Jung Kim, O.M.D., Il-Bok Seo* Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung UniversityDept. of Anatomy, College of Oriental Medicine, Se-Myung University* Objectives : This study was to investigate the effects of Prunellae Herba on Proteoglycan(PG) degradation by measuring body weight, Glycosaminoglycan(GAG), Interleukin-1β(IL-1β) in synovial fluid, and PG content of articular cartilage of femur in collagenase-induced arthritis in rats. Methods : Arthritis was induced by injection of collagenase(0.1 ml) into knee joint of rats. Arthritic rats were divided into control(n=8) and treated(n=8) group. Control group was taken normal saline for 15 days and treated group was taken extracts of Prunellae Herba for same duration. Normal group(n=8), non-arthritic group, was injected with normal saline and was taken normal saline for 15 days. Body weight was measured at 0, 10, 15 days after injection. GAG, IL-1β in synovial fluid were measured with ELISA kit at 15 days after injection. PG content of articular cartilage of femur, represented by safranine O staining, was measured at 15 days after injection. Histopathological study on the articular cartilage of knee joint was investigated at 15 days after injection. Results : Body weight of treated group was not significantly increased, PG of treated group was significantly increased, and GAG was significantly decreased compared with control group. But IL-1β was not significantly decreased. Conclusions : On the basis of these results, we concluded that Prunellae Herba has inhibiting effects on the Proteoglycan degradation in collagenase-induced rat osteoarthritis model.

Effect of Nardostachytis Rhizoma on Induction of Differentiation in HL-60 Cells Sang-Hak Yun, O.M.D., Jong-Ha Park, O.M.D., Kyeong-Su Kim, O.M.D., Won-Kyo Oh, O.M.D., Seung-Ryong Yeom, O.M.D., Young-Dal Kwon, O.M.D., Yung-Sun Song, O.M.D. Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University Objectives : To investigate PKC/MAPKs signaling pathways on the human promyelocytic leukemia HL-60 cell differentiation induced by Nardostachytis Rhizoma (N. Rhizoma). Methods : Differentiation of HL-60 cells was determined through expression of CD11b and CD14 surface antigens using flow cytometry and nitroblue tetrazolium (NBT) assay. PKC activity was measured by using a PKC Kinase Activity Assay kit (Non-Radioactive). Activation of ERK and protein levels of PKC isoforms were determined by Western blot analysis. Results : Activation of extracellular signal-regulated kinase (ERK) increased time-dependently in differentiation of HL-60 cells induced by N. Rhizoma. Inhibitor of ERK (PD98059) significantly reduced NBT reduction activity induced by N. Rhizoma in HL-60 cells. In addition, N. Rhizoma increased protein level of PKC α, PKC βI and PKC βII isoforms, but the protein level of PKC γ was constant. PKC inhibitors (GF 109203X, Chelerythrine and H-7) inhibited HL-60 cell differentiation and the ERK activation induced by N. Rhizoma. Conclusions : N. Rhizoma induces granulocytic differentiation via a PKC/ERK signaling pathway in HL-60 cells.

Effects of Bee Venom Treatment on the Injured Ligament Seung-Min Lee, O.M.D., Dong-Ho Keum, O.M.D. Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Guk University 목적 : 손상된 인대조직이 염증기(Inflammation phase)에서 재형성기(Remodeling phase)로 이행되는 과정에서 봉독요법 치료가 손상 인대조직의 회복에 미치는 영향을 생화학적, 조직학적 방법으로 세포와 분자적인 관점에서 연구하고자 하였다. 방법 : 우측 뒷발의 족관절 염좌를 유발한 쥐들을 4주와 8주 후, 매주 1회 6주간 환측 족관절 전외측 부위(GB40, 구허)에 봉독요법을 시행한 후 인대조직을 박리하여 Western blotting, Immunofluorescence staining과 RT-PCR을 통하여 단백을 분석하였다. Hoechst 핵 염색법으로 세포수를 측정하였고 Masson's trichrome 염색법으로 콜라겐 섬유를 시각화하였다. 결과 : 봉독실험군에서 phospho-Erk1/2 kinase가 같은 기간 동안 손상대조군에 비해 증가되었다. 증가된 Erk1/2의 활성도는 Cyclin B1 단백질의 증가와 양성적인 관련을 보였고, 전체 세포수도 대조군보다 많았다. Type III collagen mRNA와 단백질은 봉독실험군에서 증가되었고 Trichrome staining에서도 증가된 collagen fiber를 확인할 수 있었다. TNF-α와 IL-1α mRNA 수준은 봉독실험군에서 손상대조군에 비해 현저한 감소를 보였다. 결론 : 인대 손상에 봉독요법을 시행한 결과 세포 증식 및 증가된 세포 활성을 통해 인대조직의 회복을 촉진함을 예측할 수 있었다.

The Study on Pillow Height for Admission Patients to the Oriental Medical Hospital due to Stroke Jung-Hun Nam, O.M.D., Sung-Woo Cho, O.M.D., Gyung-Hoon Ko, O.M.D., Bong-Hyun Kim, O.M.D., In-Seon Lee, O.M.D. Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Eui University Objectives : The purpose of this study is to investigate the relation of pillow height for stroke patients. Methods： One hundred fourteen stroke patients were enrolled in this clinical study. The height of pillow was measured from the datum surface to occipital protuberance after 5 minutes in supine position. This study is an attempt to analyse the relation between the pillow height and the angle of cervical spine to shoulder joint, and compare the result with normal subjects. Results： The average height of pillow was 11.32±2.66cm, and this value is much higher than 5.0±10cm which is claimed to be the ideal height from some previous studies, and also greater than 10.1cm which is the average pillow height from other related studies. Logistic regression was used to determine the influence of various elements, and one where the group was divided into male and female seemed to show significant difference on the pillow height, where preceding diseases seemed to have no effect either on the pillow height nor the curvature of the cervical spine, which had the average value of 32.03±6.04°. Conclusions： This results suggest that stroke patients use higher pillows than normal subjects. More studies are required about the effect of pillow height on cerebral blood flow and stroke, and the method of measuring the pillow height and the curvature of cervical spine.

Study of Relationship between Autonomic Nerve System and Post Stroke Insomnia by Heart Rate Variability Jae-Heung Cho, O.M.D., Dong-Jae Shin, O.M.D., Jong-Soo Lee, O.M.D., Sung-Soo Kim, O.M.D. Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyung-Hee University Objectives : This study was designed to evaluate the effect of imbalance of autonomic nerve system on post-stroke patients with insomnia by Heart Rate Variability parameters. Methods : Sample group of 15 patients with post stroke insomnia was tested Heart Rate Variability parameters which are Mean Heart Rate(MHRT), Standard Deviation of all the Normal RR intervals(SDNN), Root Mean Square of Successive Differences Between The Normal Heart Beats(RMSSD), Total Power(TP), Low Frequency(LF), High Frequency(HF) and LF/HF ratio. This results were compared with that of control group including 15 patients without post stroke insomnia. Results : 1. MHRT and LF/HF ratio were not significantly different between sample group and control group(P=0.061, 0.305). 2. SDNN and RMS-SD were significantly different between sample group and control group(P=0.013, 0.029). 3. TP, HF and LF of sample group were significantly different compared with those of conrol group(P=0.023, 0.037, 0.037). Conclusions : General parameters of Heart Rate Variability except MHRT and LF/HF ratio were dropped off in activity. This result means that post stroke insomnia was affected more by secondary factors than simple stress due to environmental change.

Relationship of Heart Rate Variability with Body Mass Index and Waist Circumference in Premenopausal Women Ae-Ri Han, O.M.D., Mi-Yeon Song, O.M.D., Hyun-Dae Shin, O.M.D. Dept.of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyung-Hee University Objectives : The purpose of this study is to investigate the relationship between obesity parameters(Body Mass Index and Waist Circumference) and Heart Rate Variability(HRV) in premenopausal women. Methods : The study was performed in 71 premenopausal women from 25 to 44 year-old who visited to an oriental medical clinic in, height and weight were measured using electronic instrument. To measure autonomic nerve activity, 5-minute electrocardiographic recordings were analyzed with time and frequency domain methods of Heart Rate Variability(HRV). Results : 1. RMSSD(the square root of the mean squared differences of successive normal-to-normal intervals) and Ln HF(high frequency power) showed significant correlation with Body Mass Index. Correlation coefficience of RMSSD and Ln HF with Body Mass Index was r=-0.260(p<0.05) and r=-0.343(p<0.01) respectively. 2. SDNN(the standard deviation of all the normal RR interval), RMSSD, Ln TP(total power) and Ln HF showed significant correlation with Waist Circumference. Correlation coefficience of SDNN, RMSSD, Ln TP and Ln HF with Waist Circumference was r=-0.255(p<0.05), r=-0.343(p<0.01), r=-0.256(p<0.05), and r=-0.395(p<0.01) respectively. Conclusions : There was a significant relationship of Heart Rate Variability(HRV) with obesity parameters(Body Mass Index and Waist Circumference) in premenopausal women. As Body Mass Index and Waist Circumference were increased, activity of the autonomic nerve system was decreased, especially parasympathetic activity was significantly decreased.

A Case Report of a Patient with Facial Nerve Paralysis Caused by Traumatic Temporal Bone Fracture Hye-Lim Ahn, O.M.D., Mi-Suk Shin, O.M.D. Dept. of Oriental Rehabilitation Medicine, Dong-Shin University Mok-Dong Oriental Hospital Objectives : The purpose of this study is to report that a patient with facial nerve paralysis caused by traumatic temporal bone fracture was improved by non-surgical treatment. Methods : We treated this patient with electric acupuncture, physical therapy and herbal medication. We evaluated the state of improvement by House-Brackmann facial grading scale, Yanagihara's unweighted grading system, DITI(digital infrared thermographic imaging) and EMG(electromyography). DITI was measured twice before treatment and after 25 weeks ; EMG before treatment and after 12 weeks. Results : The subject was improved in estimation by House-Brackmann facial grading scale, Yanagihara's unweighted grading system, DITI and EMG. Conclusions : Although this case was supposed to take surgical treatment, non-surgical treatment(electric acupuncture, physical therapy and herbal medication) was effective to improve many symptoms of facial nerve paralysis. We expect we can use non-surgical treatments more broadly for diseases which can be taken only by surgery with subsequent studies.