The Effects of Dokhwalgisaeng-tang(Duhuoqisheng-tang) and Jungsongouhyul Pharmacopuncture on Pain Control and Nerve Regeneration in the Crush-induced Sciatic Nerve Injury of the Rat Model

이상규; 원재균; Seung-ryong Yeom; LEE SU KYUNG; SONG YUNG SUN; Young Dal Kwon

Objectives : The purpose of this Study was to identify the effect of Dokhwalgisaeng-tang(Duhuoqisheng-tang) and Jungsongouhyul pharmacopuncture on pain control and nerve regeneration after crush injury in rat sciatic nerve. Methods : Neuropathic pain was induced by crush-induced model of right sciatic nerve. Animal groups were divided as follows; Group Ⅰ: no treatment control group, Group Ⅱ : experimental group treated with Dokhwalgisaeng-tang(Duhuoqisheng-tang), Group Ⅲ : experimental group treated with Jungsongouhyul pharmacopuncture, and Group Ⅳ : experimental group treated with Dokhwalgisaeng-tang(Duhuoqisheng-tang) and Jungsongouhyul pharmacopuncture. To evaluate pain intensity, each group was observed paw withdrawal threshold and immunoreactivity on the c-fos before and after respective treatments in five hours, first, third, and fifth day. To evaluate nerve regeneration, those were observed SFI(Sciatic Functional Index) and GAP-43(Growth Associated Protein 43) after each treatment in seventh and thirteenth day. Results : 1. Paw withdrawal threshold to the mechanical stimuli made the significant difference between group Ⅳ and the control group after five days of the experiment. 2. Paw withdrawal threshold to the thermal stimuli made the significant difference between group Ⅰ and Ⅲ, Ⅰ and Ⅳ, Ⅱ and Ⅳ. 3. In immunohistochemical response of c-fos, as time passes, the immunoreactivity of all groups was decreased gradually. Especially, group Ⅳ was observed the lowest after three days. 4. The differences of sciatic function indexes in each group were significantly between group Ⅰ and Ⅲ, Ⅰ and Ⅳ, Ⅱ and Ⅳ after 14 days, and between group Ⅰ and Ⅲ, Ⅰ and Ⅳ, Ⅱ and Ⅳ, Ⅲ and Ⅳ after 21 days. 5. In immunohistochemical response of GAP-43, all groups had higher GAP-43 immunoreactivity at the 14 days from post-injury and group Ⅳ showed highest immunoreactivity. Conclusions : Based on above the results, it is proposed that Dokhwalgisaeng-tang(Duhuoqisheng-tang) and Jungsongouhyul pharmacopuncture may be helpful as a treatment in neuropathic pain and nerve regeneration in rat model.

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* 2022년 이후 발행 논문의 참고문헌은 현재 구축 중입니다.

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