Effect of Fermented Black Rice Extract on NH4Cl and Acetaminophen-induced Hepatotoxicity

Jong-Min Park; 박선옥; LEE SANG JONG; Shambhunath Bose; 이수진; Jeon， Song-Hee; 송미영; Hojun Kim; Myeong-Jong Lee

Journal of Korean Medicine Rehabilitation

Abbr : JKMR
2012, 22(2), pp.31-45
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Korean Medicine

Jong-Min Park ¹ , 박선옥 ² , LEE SANG JONG ³ , Shambhunath Bose ⁴ , 이수진 ⁵ , Jeon， Song-Hee ⁶ , 송미영 ⁷ , Hojun Kim ⁸ , Myeong-Jong Lee ⁹

¹ 동국대학교 한방재활의학과 교실
² (주)에스티알바이오텍
³ (주)에스티알바이오텍
⁴ Dongguk University
⁵ 동국대학교 한방재활의학과 교실
⁶ 동국대학교
⁷ 동국대학교
⁸ 동국대학교
⁹ 동국대학교

ABSTRACT

Objectives :This study was carried out to investigate the hepato-protective effects of fermented black rice(Oryza sativa Linne) extracts(FBRE) through of HepG2 cell and rats. Methods :In vitro, the cell viability, ammonia level, liver enzymes including AST, ALT, GST and SOD were investigated after the treatment of fermented black rice extract(FBRE) and positive controls of commercial nutraceutical products in NH4Cl-treated HepG2 cell lines. In vivo, liver function biomarkers were measured in acetaminophen hepatotoxicity animal model. 32 male rats were divided into four groups and each group were fed different formula for 4 days, i.e, normal control(C), acetaminophen treatment(AA), Hepakhan 250 mg/kg(commercial product as positive control) + acetaminophen treatment(HK+AA), fermented black rice extracts 250 mg/kg + acetaminophen treatment(FBRE+AA). Weight, food intake, liver enzymes, lipid-protein profiles were investigated. Results :In vitro, FBRE and two commercial products(Hepaglucan and Helpkhan) had shown to improve the cell viability, ammonia level, GPT, SOD in HepG2 cell and there were, however, no change on GST, GOT observed. FBRE had shown that similar or better hepato-protective effects than other commercial products. In vivo experiment, there were no significant change in food intake but body weight decreased in all acetaminophen-treatment groups(p<0.05). GPT and glucose level was significantly decreased in FBRE+AA group(p<0.05). Conclusions :These results suggest that FBRE has potential possibility to improve compromised hepatic function.
KEYWORDS

hepato-protective effects , fermented black rice(Oryza sativa Linne) extracts , HepG2 cell , rats liver cell.
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