Effect of Fermented Black Rice Extract on NH4Cl and Acetaminophen-induced Hepatotoxicity

Jong-Min Park; 박선옥; LEE SANG JONG; Shambhunath Bose; 이수진; Jeon， Song-Hee; 송미영; Hojun Kim; Myeong-Jong Lee

Journal of Korean Medicine Rehabilitation

Abbr : JORM
2012, 22(2), pp.31-45
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Korean Medicine

Jong-Min Park ¹, 박선옥 ², LEE SANG JONG ³, Shambhunath Bose ⁴, 이수진 ⁵, Jeon， Song-Hee ⁶, 송미영 ⁷, Hojun Kim ⁸, Myeong-Jong Lee ⁹

¹동국대학교 한방재활의학과 교실
²(주)에스티알바이오텍
³(주)에스티알바이오텍
⁴Dongguk University
⁵동국대학교 한방재활의학과 교실
⁶동국대학교
⁷동국대학교
⁸동국대학교
⁹동국대학교

ABSTRACT

Objectives :This study was carried out to investigate the hepato-protective effects of fermented black rice(Oryza sativa Linne) extracts(FBRE) through of HepG2 cell and rats. Methods :In vitro, the cell viability, ammonia level, liver enzymes including AST, ALT, GST and SOD were investigated after the treatment of fermented black rice extract(FBRE) and positive controls of commercial nutraceutical products in NH4Cl-treated HepG2 cell lines. In vivo, liver function biomarkers were measured in acetaminophen hepatotoxicity animal model. 32 male rats were divided into four groups and each group were fed different formula for 4 days, i.e, normal control(C), acetaminophen treatment(AA), Hepakhan 250 mg/kg(commercial product as positive control) + acetaminophen treatment(HK+AA), fermented black rice extracts 250 mg/kg + acetaminophen treatment(FBRE+AA). Weight, food intake, liver enzymes, lipid-protein profiles were investigated. Results :In vitro, FBRE and two commercial products(Hepaglucan and Helpkhan) had shown to improve the cell viability, ammonia level, GPT, SOD in HepG2 cell and there were, however, no change on GST, GOT observed. FBRE had shown that similar or better hepato-protective effects than other commercial products. In vivo experiment, there were no significant change in food intake but body weight decreased in all acetaminophen-treatment groups(p<0.05). GPT and glucose level was significantly decreased in FBRE+AA group(p<0.05). Conclusions :These results suggest that FBRE has potential possibility to improve compromised hepatic function.
KEYWORDS

hepato-protective effects, fermented black rice(Oryza sativa Linne) extracts, HepG2 cell, rats liver cell.
REFERENCES (53)

[journal] Sung KS / 2000 / Effects of red ginseng component administration on glutathione and lipid perocidation levels in mice liver / J. Ginseng Res 24(4) : 176~182

[journal] 정재연 / 2002 / Suppressive Effects of Antioxidant DA-9601 on Hepatic Fibrosis in Rats / Clinical and Molecular Hepatology / 대한간학회 8(2002) : 436~447

[journal] Cha BC / 2000 / Antioxidative and hepatoprotective effect of compounds isolated from Galla Rhois(Rhus javanica Linne) / Medical Crop sci 8(2) : 157~164

[thesis] 정준학 / 2007 / 사염화탄소에 의해 유도된 흰쥐의 간손상에 대한 헛개나무 메탄올 추출물의 보호효과 / 석사 / 대구가톨릭대학교 대학원

[thesis] 이민아 / 2009 / 사방오리나무의 간섬유화 억제활성 성분 / 석사 / 서울대학교 대학원

[thesis] 이성현 / 2008 / 고려엉겅퀴의 항산화 및 간 보호활성과 Syringin의 분리 / 석사 / 강원대학교 대학원

[journal] 문진영 / 2002 / 월국환 메탄올 추출물이 산화적 간손상에 미치는 효과 / 대한한의학방제학회지 10(2) : 85~95

[journal] 박선동 / 2000 / 실소산이 CCl4 유도 간손상 Mouse의 기능회복에 미치는 영향 / 대한한의학방제학회지 8(1) : 169~190

[book] Ramarathnam N / 1986 / Studies on the relationship between antioxidative activity of rice hull and germination ability of rice seed / J. of Food and Agriculture : 719~726

[journal] 지희연 / 2005 / 자광찰벼 추출물의 H4IIE 세포에서의 항산화 효과 / Korean J. Crop. Sci 50 : 8~11

[journal] 강미영 / 2008 / Effects of Rice Embryo and Embryo Jelly with Black Rice Bran Pigment on Lipid Metabolism and Antioxidant Enzyme Activity in High Cholesterol-Fed Rats / Journal of the Korean Society for Applied Biological Chemistry / 한국응용생명화학회 51(3) : 200~206

[journal] Choi SW / 1996 / Antioxidative activity of ethanolic extracts of rice brans / Food Biotechnol 5 : 305~309

[journal] Tsuda T / 1994 / Antioxidative activity of the anthocyanin pigments cyanidin 3-O-β-Dglucoside and cyanidin / Food Chem 42 : 2407~2411

[journal] Nam SH / 1997 / In vitro inhibitory effect of colored rice bran extracts carcinogenicity / Agric. Chem. Biotechnol 40 : 307~312

[journal] Nam SH / 1998 / Comparison of effect of rice bran extracts of the colored rice cultivars on carcinogenesis / Agri. Chem. Biotechnol 41 : 78~83

[journal] Hamuro J / 1980 / Induction of cytotoxic peritional exdudate cells by T-cell immune adjuvants of the (1-3)glucan type letinan and anloguses / Immunology 39 : 229~

[thesis] 김우섭 / 2007 / 버섯 추출물을 이용한 육제품 개발에 관한 연구 / 석사 / 대구한의대학교 대학원

[thesis] 박승용 / 2009 / 복분자로 배양된 표고버섯 균사체 추출물의 항암 및 항산화 효과 검증 / 석사 / 대구한의대학교 한방산업대학원

[book] 김윤숙 / 2003 / 미강발효액의 생리활성 및 실용화 / 한국식품개발연구원

[thesis] 이성규 / 2009 / 녹두를 첨가한 탈지대두 발효물의 콜레스테롤 및 지질대사 개선에 미치는 영향 / 석사 / 계명대학교 대학원

[journal] Guengerich FP / 1991 / Role of human cytochrome P-450 IIEL in the oxidation of many low molecular weight cancer suspects / Chem. Res. Toxicol 4 : 168~179

[journal] Imaeda AB / 2009 / Acetaminophen-induced hepatotoxicity in mice is dependent on Tlr9 and the Nalp3 inflammasome / J Clin Invest 119 : 305~314

[journal] Hinson JA / 2010 / Mechanisms of acetaminophen-induced liver necrosis / Handb Exp Pharmacol 196 : 369~405

[journal] Reitman G / 1957 / A method of determining serum GOT and GPT activity / Amer. J. Clin. Path 28 : 58~62

[book] Institute of laboratory Animal Resources / 1996 / Guide for the Care and Use of Laboratory Animals / National Academy Press

[journal] Cabaud PG / 1958 / Colorimetric measurement of lactic dehydrogenase activity of body fluids / American journal of clinical pathology 30(3) : 234~236

[journal] Daniel O / 1954 / Urinary excretion of acid phosphatase / British medical journal 1(4) : 19~21

[journal] Raabo E / 1960 / On the enzymatic determination of blood glucose / Scandinavian journal of clinical & laboratory investigation 12(4) : 402~407

[journal] Allain CC / 1974 / Enzymatic determination of total serum cholesterol / Clinical chemistry 20(4) : 470~475

[book] 안덕균 / 2002 / 한국본초도감 / 교학사 : 480~

[other] 통계청 / 2009년 연령별 사망원인 순위 / 연도별 사망원인 순위

[book] 허준 / 2005 / 동의보감 : 2004~2007

[book] 전국한의과대학 본초학교실 / 1994 / 본초학 / 영림사 : 372~373

[book] 진존인 / 1982 / 중약대사전 / 강담사 : 204~

[journal] Choi HC / 1996 / Diversity and function of pigments in colored rice / Korean J. Crop 41 : 1~9

[journal] 남석현 / 1997 / 유식미 쌀 추출겨의 in vitro의 발암 억제 효과 / Agri. Chem. & Bio 40(4) : 307~312

[thesis] 김재운 / 2001 / 흑미(Oryza sativa L.)의 물리화학적 성질 및 항산화성 / 석사 / 고려대학교 대학원

[journal] 정용준 / 2007 / Effect of Fermented Herbal Extracts, HP-1 on Enzyme Activities and Gene Expressions Related to Alcohol Metabolism in Ethanol-loaded Rats / 동의생리병리학회지 / 대한동의병리학회 21(2) : 387~391

[thesis] 김용미 / 2009 / 발효한약의 연구동향 연구 / 석사 / 경원대학교 대학원

[journal] Duthie SJ / 1989 / The role of reductive and oxidative metabolism in the toxicity of mitooxantrone, adriamycin and manadione in human liver derived HepG2 hepatoma cells / Br. J. Cancer 60 : 566~571

[journal] Haussinger D / 2002 / Pathogenesis of hepatic encephalopathy / J. Gastroenterol. Hepatol 17(3) : 256~259

[journal] Jakoby WB / 1978 / The glutathione S-transferase : a group of multi-functional detoxification proteins / Adv Enymol Relat Areas Mol Bio 46 : 383~414

[journal] Charles / 2006 / Clinical and experimental approaches to the pathophysiology of interleukin-18 in cancer progression / Cancer and Metastasis Reviews 25(3) : 417~434

[book] Lieber CS / 1985 / Alcohol and the liver, metabolism of ethanol, medtabolism effects and pathogenesis of injury / Acta. Med. Scand. Suppl : 11~55

[journal] 김병로 / 2004 / The Effect of Short term Vitamin E Supplementation on the Lipid Peroxide and SOD, CAT Activity after Submaximal Exercise. / 한국체육학회지 / 한국체육학회 43(2) : 443~452

[thesis] 이문희 / 2008 / 사삼의 아세트아미노펜 유발 간손상 보호효과 / 박사 / 대구가톨릭대학교 대학원

[journal] Gerard MJ / 1997 / Serum glucose levels and alcohol consumption habits in a large population / Diabetes 26 : 780~

[journal] Inkeles S / 1981 / Hyperlipidemia and coronary atherosclerosis / Medicine 60 : 110~123

[other] Manninen V / Triglycerides and LDL-cholesterol concenttration on coronary heart disease risk in the Helsinki Heart Study

[journal] Kim BK / 2001 / Cholesterol-lowering effect of mushrooms powder in hyperlipidemic rats / J Korean Soc Food Sci Nutr 30 : 510~515

[journal] Cha JY / 2000 / Effect of water extract of leaves from Morus alba and Cudrania tricuspidata on the lipid concentration of serum and liver in rats / Agric Chem. Biotechnol 43 : 303~308

[journal] 강미영 / 2008 / Effects of Rice Embryo and Embryo Jelly with Black Rice Bran Pigment on Lipid Metabolism and Antioxidant Enzyme Activity in High Cholesterol-Fed Rats / Journal of the Korean Society for Applied Biological Chemistry / 한국응용생명화학회 51(3) : 200~206

[thesis] 이상관 / 2005 / 사염화탄소 투여로 유발된 간 손상에 대한 인진쑥 추출물의 치료효과 / 박사 / 경북대학교 대학원
KCI Citation (0)

KJC Korea
Journal Central

Statistics

Tools