Anti-Obesity Effect of Wild Ginseng Complex Pharmacopuncture on Adipocyte and High Fat Diet-Induced Obese C57BL/6J Mice
2012, 22(2), pp.67-90
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Korean Medicine
-
Objectives : The study was designed to experiment the anti-obesity and anti-inflammatory effects of wild ginseng complex pharmacopuncture which was made with mixture of Wild ginseng, Bovis Calculus, Ursi Fel and Moschus to the adipocyte and C57BL/6J mice which was induced obesity with high-fat diet. Methods : We treated wild ginseng complex pharmacopuncture to NIH/3T3 mouse fibroblast(3T3-L1) and Raw264.7 mouse macrophage(macrophage cell). We measured cytotoxicity using MTT assay principles. We tried to find out anti-obesity effect by measuring NO production, adiponectin, PPAR-γ expression and Oil Red O experiment. And by measuring NO production, iNOS, COX-2 and NF-κB expression, we tried to find out anti-inflammatory effect. In animal experiments, subject groups were divided into normal diet group, high fat diet group, and high fat diet and wild ginseng complex pharmacopuncture in BL23 treated group. We observed the changes of body weight, organ’s weight, AST, ALT, γ-GTP, BUN, total cholesterol, HDL cholesterol, LDL-cholesterol, Plasma insulin, leptin. Results : As a result of treating wild ginseng complex pharmacopuncture, there was no cytotoxicity except 50% concentration. When wild ginseng complex pharmacopuncture was treated to 3T3-L1 which is preadipocyte, it has an anti-obese effect. In case of treating wild ginseng complex pharmacopuncture to macrophage cell, we could found out anti-inflammatory effect. After injecting wild ginseng complex pharmacopuncture in corresponding part of BL23 for 6 weeks' mice, it was identified that it has an efficacy of restraining a body weight gain. After 6 weeks, as a result of measuring the weight of mice's liver, kidney and epididymis, the weight reduced comparing with high fat diet group, so it came out that it has prevention effect in accumulation of fats. The AST, ALT, γ-GTP, BUN level were lower in group of wild ginseng complex pharmacopuncture than the group of high fat diet. After measuring blood lipids, total cholesterol and LDL-cholesterol level were normal in group of wild ginseng complex pharmacopuncture and HDL cholesterol level were higher than the group of high fat diet. Concentrations of Plasma insulin and blood leptin were lower in group of wild ginseng complex pharmacopuncture than the group of high fat diet. Conclusions :From all these results, the wild ginseng complex pharmacopuncture showed the significant efficacy as a obesity treatment and it is thought as a safe treatment that could expect even the efficacy of anti-inflammatory.
-
[journal] Nielsen S / 1997 / Obesity and cardivascular disease is body structure a factor / Curr. Opin. Lipidol 8 (4) : 200~204
[journal] 이기업 / 198805 / ARTIFICIAL INDUCTION OF INTRAVASCULAR LIPOLYSIS BY LIPID-HEPARIN INFUSION LEADS TO INSULIN RESISTANCE IN MAN / DIABETOLOGIA / SPRINGER VERLAG 31 (5) : 285~290
[journal] Kissebah AH / 1976 / Role of insulin resistance in adipose tissue and liver in the pathogenesis of endogenous hypertriglyceridaemia in man / Diabetologia 12 (6) : 563~571
[journal] Mertens IL / 2000 / Overweight, obesity, and blood pressure : the effects of modest weight reduction / Obes Res 8 (3) : 270~278
[journal] American Diabetes Association / 2003 / Clinical practice recommendations / Diabetes Care 26 (2) : 21~24
[journal] Must A / 1999 / The Disease Burden Associated With Overweight and Obesity / JAMA 282 (16) : 1523~1529
[journal] Ro YM / 2004 / Metabolic syndrome and cardiovascular disease / J. of KAHP 2 (1) : 72~77
[journal] Kopelman PG / 2000 / Obesity as a medical problem / Nature 404 (1) : 635~643
[book] 보건복지부 / 2010 / 2010 국민건강통계 / 보건복지부 건강정책과 : 52~53
[book] 한방재활의학과학회 / 2006 / 한방재활의학 / 군자출판사 : 390~396
[journal] 황덕상 / 2006 / 저주파 전침 자극이 허벅지 둘레에 미치는 영향 / 대한한방비만학회지 5 (1) : 1~8
[journal] Miwa H / 2002 / Effect of ultrasound application on fat mobilization / Pathophysiology 9 (1) : 13~19
[journal] Alster TS / 2005 / Cellulite treatment using a novel combination radiofrequency, infrared light and mechanical tissue manipulation device / J Cosmet Laser Ther 7 (2) : 81~85
[journal] 김민우 / 2011 / Study of Experimentations and Clinical Trials' Trends for Obesity Treatment using Pharmacupuncture / 한방비만학회지 / 한방비만학회 11 (1) : 47~60
[journal] Susumu Kohno / 2011 / a novel inhibitor of nitric oxide production in mouse macrophage RAW264.7 cells / Journal of natural medicines 65 (2) : 353~359
[journal] Kurulgan Demirci E / 2010 / Genome-Wide Gene Expression Analysis of NIH 3T3 Cell Line Under Mechanical Stimulation / Cellular and Molecular Bioengineering 4 (1) : 46~55
[journal] Sankara Ghosh / 1990 / Activation in vitro of NF-κB"by phosphorylation of its inhibitor κB" / Nature 344 (6267) : 678~682
[journal] Antonella Franchini / 2011 / Tail regenerative capacity and iNOS immunolocalization in Xenopus laevis tadpoles / Cell and Tissue Research 344 (2) : 261~269
[journal] Nguyen Bich Thu / 2010 / Zanthoxylum rhetsa Stem Bark Extract Inhibits LPS-induced COX-2 and iNOS expression in RAW 264.7 Cells via the NF-kB Inactivation / Natural Product Sciences / 한국생약학회 16 (4) : 265~270
[journal] Li-na L / 2009 / Detection of an angiotensin converting enzyme inhibitory peptide from peanut protein isolate and peanut polypeptides by western blot and dot blot hybridization / European Food Research and Technology 230 (1) : 89~94
[journal] 강수우 / 2007 / Effects of Herbal Acupuncture(Raphanus Sativus) at BL20 on the Hyperlipemia Rats Induced by High Fat Diet / 대한침구의학회지 / 대한침구의학회 24 (5) : 113~125
[journal] Allain CC / 1974 / Enzymatic determination of total serum cholesterol / Clin Chm 20 (4) : 470~475
[journal] McGowan MW / 1983 / A peroxidase-coupled method for the colorimetric determination of serum triglycerides / Clin Chem 29 (3) : 538~542
[journal] Friedewald WT / 1972 / Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge / Clin Chem 18 (6) : 499~502
[journal] Sjostrom CD / 1997 / Relationships between changes in body composition and changes in cardiovascular risk factors : the SOS Intervention Study / Obes Res 5 (6) : 519~530
[journal] 안병철 / 2005 / Socioeconomic Cost of Obesity in Korea / Journal of Nutrition and Health / 한국영양학회 38 (9) : 786~792
[journal] 주집성 / 2003 / 비만 치료를 위한 경혈 및 약물에 관한 고찰 / 대한경락경혈학회지 20 (3) : 147~162
[journal] 권기록 / 2010 / Anticancer effect of mountain ginseng Pharmacopuncture to the nude mouse of lung carcinoma induced by NCI-H460 human non-small cell lung cancer cells / Journal of Pharmacopuncture / 대한약침학회 13 (1) : 5~14
[journal] 장성봉 / 2010 / Anti-metastatic mechanism of mountain cultivated wild ginseng in human cancer cell line / Journal of Pharmacopuncture / 대한약침학회 13 (1) : 37~43
[journal] 이명선 / 2010 / 인체 결장암세포에서 항암제와 산삼배양근에 의한 p53 유전자 발현 / 산업과학연구 27 (2) : 25~32
[journal] 이명선 / 2010 / Cisplatin and Extract of Tissue Cultured Mountain Ginseng-Induced Apoptosis in Human Cervical Cancer Cells / 한국현미경학회지 / 한국현미경학회 40 (3) : 133~138
[journal] 이상훈 / 2007 / Effects of cultivated wild ginseng pharmacopuncture at Gansoo (BL18) and Gokji (LI11) on lipid composition, cytokine level, liver function and antioxidative capacity in diet-induced obese rats / Korean Journal of Acupuncture / 경락경혈학회 24 (4) : 181~200
[journal] Heung Mook Shin / 2009 / Mixture of Wild Panax Ginseng and Red-Mold Rice Extracts Activates Macrophages through Protection of Cell Regression and Cytokine Expression in Methotrexate-Treated RAW264.7 Cells / 대한한의학회지 / 대한한의학회 30 (6) : 69~79
[journal] 김형석 / 2007 / Protective Effect of Wild Ginseng Extract against t-BHP-induced Oxidative Damage in HepG2 Cells Using DNA Chip / Journal of Pharmacopuncture / 대한약침학회 10 (1) : 121~135
[journal] Se Na Yun / 2004 / Wild Ginseng Prevents the Onset of High-Fat Diet Induced Hyperglycemia and Obesity in ICR Mice / Archives of Pharmacal Research / 대한약학회 27 (7) : 790~796
[journal] 이동희 / 2006 / Analysis of Serum Proteom after Intravenous Injection of cultivated wild ginseng pharmacopuncture / Journal of Pharmacopuncture / 대한약침학회 9 (2) : 17~37
[journal] 홍민희 / 2008 / The Antihypertensive and Vasodilating Effects of AdventitiousRoot Extracts of Wild Ginseng / Journal of the Korean Society for Applied Biological Chemistry / 한국응용생명화학회 51 (2) : 102~107
[journal] 권선오 / 2009 / Antidepressant Effect of the Subchronic Administration of the Methanolic Extract of Wild‐ginseng and Cultivated‐ginseng in Mice Tail Suspension Test / 대한침구의학회지 / 대한침구의학회 26 (4) : 99~106
[journal] 안규성 / 2009 / Hair Loss Prevention and Hair Growth Promotion by Herb Extracts which contain the Cultured Korean Wild Ginseng / 한국인체미용예술학회지 / 한국인체미용예술학회 10 (4) : 221~226
[journal] 김성혜 / 2006 / Effects of Cultivated Wild Ginseng Pharmacopuncture on Surgically Induced Endometriosis in Rats / 대한침구의학회지 / 대한침구의학회 23 (5) : 55~67
[journal] 최은주 / 2006 / Effects of cultivated wild ginseng pharmacopuncture on lowering lipid and oxidative capacity in biochemical and molecular biological study in obese rats / Journal of Pharmacopuncture / 대한약침학회 9 (1) : 5~20
[journal] 박원필 / 2005 / Effects of distilled Cultivated Wild Ginseng Herbal Acupuncture in Rats with Diabetes Induced by High Fat Diet / Journal of Pharmacopuncture / 대한약침학회 8 (2) : 97~108
[journal] 나창수 / 1996 / 麝香.牛黃.熊膽 藥鍼이 肝損傷에 미치는 影響에 관한 硏究 / 대한한의학회지 17 (2) : 251~263
[journal] 정경연 / 1999 / 사향 우황 웅담 복합제제(OK)약침이 백서의 류마티스 관절염중 혈액학적인 변화 및 Collagen의 분포변화에 미치는 영향 / 대한침구학회지 16 (3) : 255~268
[journal] 서형식 / 2009 / The Experimental Study of Safety and Efficacy in Using Bovis Calculus Pharmacopuncture Solution as Eye Drop / Journal of Pharmacopuncture / 대한약침학회 12 (3) : 61~72
[journal] 조희철 / 2007 / 웅담과 우황약침이 지방세포 대사에 미치는 영향 / 대한침구의학회지 / 대한침구의학회 24 (4) : 125~142
[journal] 박중양 / 1997 / 사향, 우황, 삼칠근 복합방이 고지혈증 및 뇌손상에 미치는 영향 / 대전대학교 한의학연구소 논문집 14 (1) : 425~449
[journal] 나창수 / 1997 / 약침에 사용되는 우황, 웅담과 저담의 항미생물 활성에 관한 연구 / 대한침구학회지 14 (1) : 420~426
[journal] 은재순 / 1994 / 암세포주에 대한 우황 50% MeOH 엑스와 함암제의 병용효과 / 우석대학교 논문집 16 (12) : 31~52
[journal] 이휘용 / 2010 / The Effects of Cervi Pantotrichum Cornu Pharmacopuncture and Bovis calculus⋅Fel Ursi Pharmacopuncture on the Heart Rate Variability / 대한침구의학회지 / 대한침구의학회 27 (1) : 65~74
[journal] 유은주 / 2006 / The Scavenging Effect on Nitric Oxide of Calculus Bovis․Fel Ursi․Moschus Extract Solution for Herbal-acupuncture / 대한침구의학회지 / 대한침구의학회 23 (4) : 115~121
[journal] 정시영 / 2011 / The Comparative Study of Effectiveness between Acupuncture and its Cotreatment with Calculus Bovis·Fel Ursi·Moschus Pharmacopuncture on the Treatment of Acute Low Back Pain / 대한침구의학회지 / 대한침구의학회 28 (4) : 105~110
[journal] 윤대환 / 2006 / The Effect of Woohwang with Pear Phenolic compound on Blood Pressure, Plasma Renin, ANP in Hypertensive Rat Induced by 2K1C / 대한본초학회지 / 대한본초학회 21 (2) : 143~150
[journal] 나창수 / 1997 / 우황 약침의 효능 관찰을 위한 미세구조적 연구 / 대한한의학회지 18 (1) : 430~445
[journal] 위통순 / 2002 / Comparative study of herbal-acupuncture using Ursi Fel？Bovis Calculus and acupunture at ST36 and GB39 effect on Adjuvant Arthritis in rats / 대한침구의학회지 / 대한침구의학회 19 (2) : 164~176
[journal] 윤성식 / 2010 / The Experimental Study on the Antibiotic Effects of Fel Ursi Pharmacopuncture Solution on Bacterial Species which cause Keratitis / Journal of Pharmacopuncture / 대한약침학회 13 (1) : 45~52
[journal] 연광해 / 2009 / Inhibitory Effect of Bear Bile on Compound 48/80-induced Mast Cell Activation and IgE-mediated Vascular Permeability / 대한체질인류학회지 / 대한체질인류학회 22 (1) : 71~83
[journal] 한충택 / 2003 / Effect of Bear Bile on the Accumulation of Cholesterol Induced by High-Cholesterol Diet in Rats / Laboratory Animal Research / 한국실험동물학회 19 (1) : 52~60
[journal] Kun HAN / 2010 / Therapeutic Effect of Whole Bear Bile and Its Components against Croton Oil-Induced Rectal Inflammation in Rats / 응용약물학회지 / 한국응용약물학회 18 (1) : 83~91
[journal] 이진복 / 2010 / The Effects of Carthami Semen Pharmacopuncture and Bovis Calculus⋅Fei Ursi Pharmacopuncture on the Heart Rate Variability(HRV) / 대한침구의학회지 / 대한침구의학회 27 (2) : 11~21
[journal] 김욱 / 2005 / The Effects of Bear Bile on the Expression of the p53 Protein in Cancer Cell Lines / Anatomy and Cell Biology / 대한해부학회 38 (1) : 49~54
[journal] 주성수 / 2006 / 생쥐 뇌소교세포주에서 웅담추출활성성분(우르소데옥시콜린산)의항산화 및 세포보호효과 / 한국식품과학회지 / 한국식품과학회 38 (3) : 452~455
[journal] 박정훈 / 2005 / The Effect of Herbal-acupuncture using Ursi Fel into Zusanli(ST36) to Recover Function of Stratum Corneum on Mice Model after Atopic Dermatitis Elicitation. / 대한한의학회지 / 대한한의학회 26 (3) : 13~26
[journal] 최은욱 / 2000 / Mongolian Gerbil 의 뇌허혈 유발에 따른 뇌조직 손상에 있어서 사향 함유 우황청심원액과 영묘향 함유 우황청심원액의 조직학적 효능 비교 / 응용약물학회지 8 (3) : 255~261
[journal] 도원석 / 2001 / 楡白皮, 桂技, 牛膝, 峰毒 및 牛黃, 熊膽, 麝香복합제제 藥鍼이 mouse의 LPS誘發 關節炎의 血液學的 變化에 미치는 影響 / 대한침구학회지 18 (1) : 157~169
[journal] 임석린 / 2002 / 사향(麝香)의 수용성분(水容性分)이 생쥐 복강내(腹腔內)거식세포(巨食細胞)의 활성(活性)에 미치는 영향(影響) / 대한예방한의학회지 6 (2) : 147~155
[journal] 은재순 / 2003 / The Combined Effect of Moschus and Anti-tumor drug Mitomycin C / 동의생리병리학회지 / 대한동의병리학회 17 (6) : 1404~1408
[journal] 최은욱 / 2000 / 사향함유 우황청심원액과 영묘향함유 우황청심원액의 혈압강하 작용 및 적출심장에 미치는 효과에 대한 약리효능 비교 / 생약학회지 31 (3) : 310~319
[journal] Dandona P / 2001 / The suppressive effect of dietary restriction and weight loss in the obese on the generation of reactive oxygen species by leukocytes, lipid peroxidation, and protein carbonylation / J Clin Endocrinol Metab 86 (1) : 355~362
[journal] Esposito K / 2002 / Inflammatory cytokine concentrations are acutely increased by hyperglycemia in humans: role of oxidative stress / Circulation 106 : 2067~2072
[book] Choi JY / 2005 / Enhanced susceptibility to MPTP neurotoxicity in high-fat diet-induced obesity / Free Rad Biol Med : 806~816
[journal] Choi JW / 2001 / Increases in nitric oxide concentrations correlate strongly with body fat in obese humans / Clin Chem 47 (6) : 1106~1109
[journal] Touyz RM. / 2004 / Reactive oxygen species, vascular oxidative stress, and redox signaling in hypertension: what is the clinical significance? / Hypertension 44 : 248~252
[journal] Fortuno A / 2004 / Association of increased phargocytic NADPH oxidase-dependent superoxide production with diminished nitric oxide generation in essential hypertension / J. of Hypertension 22 (11) : 2169~2175
[journal] Arita Y / 1999 / Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity / Biochem Biophys Res Commun 257 (1) : 79~83
[journal] Hotta K / 2000 / Plasma concentrations of a novel, adipose-specific protein, adiponectin, in type 2 diabetic patients / Arterioscler Thromb Vasc Biol 20 : 1595~1599
[journal] Schoonjans K / 1996 / The peroxisome proliferator activated receptors(PPARS)and their effects on lipid metabolism and adipocyte differentiation / Biochim Biophys Acta 1302 (2) : 93~109
[journal] Jiang C / 1998 / PPAR-γ agonists inhibit production of monocyte inflammatory cytokines / Nature 391 (6662) : 82~86
[journal] Raju J / 2003 / Energy restriction reduces the number of advanced aberrant crypt foci and attenuates the expression of colonic transforming growth factor beta and cyclooxygenase isoforms in Zucker obese(fa/fa)rats / Cancer Res 63 (20) : 6595~6601
[journal] Hide I / 1997 / Suppression of TNF-alpha secretion by azelastine in a rat mast(RBL-2H3)cell line : evidence for differential regulation of TNF-alpha release, transcription, and degranulation / J Immunol 159 (6) : 2932~2940
[journal] Watson AM / 1999 / Effect of leptin on cytochrome P-450, conjugation, and antioxidant enzymes in the ob/ob mouse / Drug Metablism Disposition 27 (6) : 695~700
[journal] Considine RV / 1996 / Serum immunoreactive-leptin concentrations in normal-weight and obese humans / N Engl Journal Med 334 (5) : 292~295
[journal] Konukoglu D / 2004 / Relationship between plasma leptin and zinc levels and the effect of insulin and oxidative stress on leptin levels in obese diabetic patients / J Nutr Biochem 15 (12) : 757~760
[journal] 장지선 / 2006 / Effects of 12 weeks combined exercise program on body composition and atherosclerosis indices in middle-aged diabetic and obese women / 운동과학 / 한국운동생리학회 15 (1) : 105~116
[journal] Siegmund B / 2004 / Leptin receptor expression on T lymphocytes modulates chronic intestinal inflammation in mice / Gut 53 (7) : 965~972
-
239
Viewed
Print this page
Search PDF