Anti-Obesity Effect of Wild Ginseng Complex Pharmacopuncture on Adipocyte and High Fat Diet-Induced Obese C57BL/6J Mice
2012, 22(2), pp.67-90
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Korean Medicine
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Objectives : The study was designed to experiment the anti-obesity and anti-inflammatory effects of wild ginseng complex pharmacopuncture which was made with mixture of Wild ginseng, Bovis Calculus, Ursi Fel and Moschus to the adipocyte and C57BL/6J mice which was induced obesity with high-fat diet. Methods : We treated wild ginseng complex pharmacopuncture to NIH/3T3 mouse fibroblast(3T3-L1) and Raw264.7 mouse macrophage(macrophage cell). We measured cytotoxicity using MTT assay principles. We tried to find out anti-obesity effect by measuring NO production, adiponectin, PPAR-γ expression and Oil Red O experiment. And by measuring NO production, iNOS, COX-2 and NF-κB expression, we tried to find out anti-inflammatory effect. In animal experiments, subject groups were divided into normal diet group, high fat diet group, and high fat diet and wild ginseng complex pharmacopuncture in BL23 treated group. We observed the changes of body weight, organ’s weight, AST, ALT, γ-GTP, BUN, total cholesterol, HDL cholesterol, LDL-cholesterol, Plasma insulin, leptin. Results : As a result of treating wild ginseng complex pharmacopuncture, there was no cytotoxicity except 50% concentration. When wild ginseng complex pharmacopuncture was treated to 3T3-L1 which is preadipocyte, it has an anti-obese effect. In case of treating wild ginseng complex pharmacopuncture to macrophage cell, we could found out anti-inflammatory effect. After injecting wild ginseng complex pharmacopuncture in corresponding part of BL23 for 6 weeks' mice, it was identified that it has an efficacy of restraining a body weight gain. After 6 weeks, as a result of measuring the weight of mice's liver, kidney and epididymis, the weight reduced comparing with high fat diet group, so it came out that it has prevention effect in accumulation of fats. The AST, ALT, γ-GTP, BUN level were lower in group of wild ginseng complex pharmacopuncture than the group of high fat diet. After measuring blood lipids, total cholesterol and LDL-cholesterol level were normal in group of wild ginseng complex pharmacopuncture and HDL cholesterol level were higher than the group of high fat diet. Concentrations of Plasma insulin and blood leptin were lower in group of wild ginseng complex pharmacopuncture than the group of high fat diet. Conclusions :From all these results, the wild ginseng complex pharmacopuncture showed the significant efficacy as a obesity treatment and it is thought as a safe treatment that could expect even the efficacy of anti-inflammatory.
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