Protective Effect of Clematidis Radix Extract on CoCl2-induced Apoptosis in Human Neuroblastoma Cells

박정우; Lim Hyung Ho

Journal of Korean Medicine Rehabilitation

Abbr : JKMR
2014, 24(2), pp.41-50
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Korean Medicine

박정우 ¹ , Lim Hyung Ho ²

¹ 가천대학교 한의과대학 한방재활의학과교실
² 가천대학교 한의과대학 한방재활의학과교실

ABSTRACT

Objectives The purpose of this study was to evaluate the effects of Clematidis radix extract on CoCl2-induced apoptosis in SH-SY5Y human neuroblastoma cells. Methods In order to investigate the protective effect of Clematidis radix on CoCl2-induced cytotoxicity in neuronal cells, MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, DAPI(4,6-diamidino-2-phenylindoleI) staining, TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) assay, DNA fragmentation assay and western blotting were performed on SH-SY5Y human neuroblastoma cells. Results Cells treated with CoCl2 exhibited several apoptotic features, while cells pre-treated with Clematidis radix prior to CoCl2 exposure showed a decrease in the occurrence of apoptotic features. CoCl2 increased HIF-1α expression, in contrast, Clematidis radix treatment decreased CoCl2-induced HIF-1α expression. Pre-treatment with the extract of Clematidis radix suppressed Bax, cytochrome c, and caspase-3 expressions, and also increased Bcl-2 expression in SH-SY5Y human neuroblastoma cells. Conclusions These results suggest that Clematidis radix may exert a protective effect on CoCl2-induced apoptosis in SH-SY5Y human neuroblastoma cells.
KEYWORDS

Clematidis radix , CoCl2 , Hypoxia , Apoptosis , SH-SY5Y human neuro-blastoma cells
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