Effects of Bujasasim-tang Ethanol Extract on Oxidative Stress, Inflammation and Osteoarthritic Rat Model

Minseok Oh; Chang-Hoon Woo

ObjectivesThis study was performed to investigate the effects of Bujasasim-tang ethanol extract (BST) on oxidative stress, inflammation and osteoarthritic rat model.MethodsTo ensure safety of BST, heavy metal levels were measured and cytotoxicity test was done. In vitro, To evaluate antioxidative effects of BST, total phenolic contents, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2’–azino–bis-(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) scavenging activity, reactive oxygen species (ROS) levels were measured. Also, to evaluate anti-inflammatory effects of BST treated group, total nitric oxide (NO) and pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) levels were measured in lipopolysaccharide(LPS)-stimulated RAW 264.7 cells. In vivo, We injected MIA 50 μl (60 mg/ml) into knee joints of rats to induce osteoarthritis. Rats were divided into total 3 groups (normal, control, BST treated group, each n=7). Normal group was not treated at all without inducing osteoarthritis and taken normal diet. Control group was induced osteoarthritis by MIA and taken with 2 ml of distilled water once a day for 4 weeks. BST treated group was induced osteoarthritis by MIA and taken BST 2 ml (200 mg/kg/mouse) once a day for 4 weeks. We evaluated dynamic weight bearing with the Incapacitance Test Meter. At the end of experiment, the rats were sacrificed to observe the functions of liver and kidney, changes of WBC, neutrophil, lymphocyte, monocyte levels in blood, to evaluate the levels of pro-inflammatory cytokines, tissue inhibitor of metallopreteinases-1 (TIMP-1), matrix metalloproteinase-9 (MMP-9), prostaglandin E2 (PGE2), leukotriene B4 (LTB4) within serum. We observed change of articular structures by Hematoxylin & Eosin (H&E), safranin-O staining method and measured amount of cartilage by micro CT-arthrography. Statistical analysis was done by unpaired student’s t-test with significance level at p＜0.05 in SPSS 11.0 for windows.Results1. Safety of the BST was identified. 2. AST, ALT, BUN, creatinine levels of BST treated group were within normal limit. In vitro, 1. DPPH and ABTS free radical scavenging activities of BST showed dose-dependent increase. 2. ROS production were significantly decreased. 3. Total nitric oxide (NO) and IL-1β production were decreased. 4. IL-6 and TNF-α production were significantly decreased. In vivo, 1. Weight bearing ability was significantly increased. 2. WBC, neutrophil, lymphocyte, monocyte levels in blood were decreased. 3. IL-1β and TNF-α levels in serum were significantly decreased. and the IL-6 level was decreased. 4. TIMP-1, MMP-9, LTB4, PGE2 levels in serum were significantly decreased. 5. Cartilage volume of BST treated group was significantly increased. Also changes of cartilage, synovial membrane, fibrous tissue were suppressed.ConclusionsThe results obtained in this study Bujasasim-tang have effects of anti-oxidative, antiinflammatory, relieve pain and protection of cartilage. Therefore we expect that Bujasasim-tang is effective treatment for osteoarthritis. (J Korean Med Rehab 2015;25(2):15-35)

Citation status

KCI Citation Counts (4)
열림/닫힘 버튼
REFERENCES (69)
열림/닫힘 버튼
* 2021년 이후 발행 논문의 참고문헌은 현재 구축 중입니다.

[book] 한방재활의학과학회 / 2011 / 한방재활의학 제3판 / 군자출판사 : 84~98

[book] 정영복 / 2006 / 정형외과학 제 6 판 / 최신의학사 : 253~261

[book] 한태륜 / 2009 / 재활의학 / 군자출판사 : 905~936

[book] 張介賓 / 1988 / 景岳全書上 / 대성문화사 : 229~234

[journal] 김영억 / 2011 / Anti-inflammation Effect of Low Intensity Laser Therapy in Collagen-induced Arthritis in Rats / 동의생리병리학회지 / 한의병리학회 25(5) : 870~875

[journal] 송주동 / 2011 / Involvement of Peroxynitrite in NO Donor-Induced HO-1 Expression in Rat Articular Chondrocytes / 생명과학회지 / 한국생명과학회 21(4) : 486~493

[journal] 이성규 / 2011 / Antioxidant and Anti-inflammatory Activities of Extracts from Korean Traditional Medicinal Prescriptions / 한국식품과학회지 / 한국식품과학회 43(5) : 624~632

[journal] 박동수 / 2011 / Effects of Gagamsosokmyeong-tang(Jiajianxiaoxuming-tang ) Treatment on the Monosodium Iodoacetate-induced Early Stage Osteoarthritis in Rats / 한방재활의학과학회지 / 한방재활의학과학회 21(4) : 49~65

[journal] 이정민 / 2013 / Effects of Jakyakkamchobuja-tang (芍藥甘草附子湯) on Papain-induced Osteoarthritis in Mice / 대한한의학회지 / 대한한의학회 34(1) : 116~135

[journal] 장효길 / 2012 / Anti-OsteoArthritic effects of Mahwangbuja-tang(Mahuangfuzi-tang ) on Papain-Induced OsteoArthritic mice model / 한방재활의학과학회지 / 한방재활의학과학회 22(4) : 59~77

[journal] 공상은 / 2013 / Effects of Sayeok-tang on Papain-Induced Osteoarthritis in Mice / 동의생리병리학회지 / 한의병리학회 27(2) : 212~224

[journal] 박동수 / 2011 / Effects of Gyeonbi-Tang Treatment on the Monosodium Iodoacetate-induced Mild Osteoarthritis in Rats / 동의생리병리학회지 / 한의병리학회 25(1) : 84~91

[journal] 신예지 / 2010 / The Effect of Aralia Cordata Thunb and Cimicifuga Heracleifolia on Cartilage Protection by the Regulation of Metabolism in Human Osteoarthric Chondrocytes / 대한침구의학회지 / 대한침구의학회 27(4) : 39~54

[journal] 최재영 / 2012 / Effects of Kamchobuja-tang(Gancaofuzi-tang ) on Papain-Induced Osteoarthritis in Mice / 한방재활의학과학회지 / 한방재활의학과학회 22(4) : 37~57

[journal] 성영석 / 2012 / The Effect of KKBT in Papain-Induced Osteoarthritis Mice Models / 한의학연구소 논문집 / 한의학연구소 21(1) : 33~52 / http://dx.doi.org/

[journal] 박동수 / 2007 / Effects of Bee-venom Treatment on the Expression of NOS, C-fos, Serotonin and Substance P in Central Nerve System of Monosodium Iodoacetate-induced Osteoarthritic Pain Model / 한방재활의학과학회지 / 한방재활의학과학회 17(3) : 1~18

[journal] Kang Mi-suk / 2008 / Effect of Ulmus Davidiana Planch Herbal Acupuncture Solution on Inhibition of Experimental Bone Resorption in Mouse Calvarial Bone Cells / 대한침구의학회지 / 대한침구의학회 25(2) : 119~127

[journal] 김현수 / 2006 / Effects of Aconitum pseudo-laeve on Nitric Oxide Synthase in the Periaqueductal Gray of Collagenase-induced Rat Osteoarthritis Model / Korean Journal of Acupuncture / 경락경혈학회 23(1) : 89~96

[journal] 박기범 / 2006 / Study on Clinical Effects of Homnis Placenta Herbal Acupuncture on Osteoarthritis of Knee Joint / 대한침구의학회지 / 대한침구의학회 23(4) : 163~173

[journal] 김용문 / 2007 / Effects of Bee-venom Treatment on the Monosodium iodoacetate-induced Osteoarthritis in Rats / 동의생리병리학회지 / 한의병리학회 21(5) : 1154~1162

[journal] Yong-Hyeon Baek / 2007 / Effect of Aralia Cordata Pharmacopuncture on Cartilage Protection and Apoptosis Inhibition In Vitro and in Collagenased-induced Arthritis Rabbit Model / 대한한의학회지 / 대한한의학회 28(4) : 114~123

[journal] 이태호 / 2009 / Effect of Trachelospermi Caulis Herbal-acupuncture on the Collagen-induced Arthritis in Rats / 대한침구의학회지 / 대한침구의학회 26(6) : 51~65

[journal] 김환영 / 2010 / Effects of Bee Venom and Cervi Cornu Parvum Pharmacoacupuncture in Monosodium Iodoacetate(MIA)-Induced Osteoarthritis Rat / 한방재활의학과학회지 / 한방재활의학과학회 20(1) : 61~77

[journal] 김은정 / 2011 / Regulation of Inflammatory Cytokine Production by Bee Venom in Rat Chondrocytes / 동의생리병리학회지 / 한의병리학회 25(1) : 132~137

[journal] 박영배 / 1995 / 施灸方法이 Adjuvant 관절염 흰쥐의혈액에 미치는 영향 / 대한침구학회지 11(2) : 137~150

[journal] 서병관 / 2011 / The Analgesic Effect and the Mechanism of Electroacupuncture on Thermal Hyperalgesia in the Rat Model of Collagenase-induced Arthritis: Mediation by Adrenergic Receptors / 대한침구의학회지 / 대한침구의학회 28(2) : 57~67

[book] 채인식 / 1971 / 傷寒論譯詮 / 고문사 : 123~

[book] 문준전 / 1990 / 동의병리학 / 고문사 : 397~398

[book] 전국한의과대학 본초학 공동교재편찬위원회 / 2010 / 본초학 / 영림사 : 216~221

[book] 이숭인 / 2008 / 古方撰次 / 校正？書局 : 166~

[journal] Altman R / 1986 / Development of criteria for the classification and reporting of osteoarthritis. Classification of osteoarthritis of the knee / Arthritis Rheum / Diagnostic and Therapeutic Criteria Committee of the American Rheumatism Association 29(8) : 1039~1049

[journal] Iannone F / 2003 / The pathophysiology of osteoarthritis / Aging Clin Exp Res 15(5) : 364~372

[book] 배병철 / 1994 / 今釋黃帝內經素問 / 성보사 : 376~382

[journal] 김태은 / 2004 / Screening of the Biologoical Activity from Water Extractsof the Medicinal Plants and the Protective Effect of R. palmatum on MTPT-induced Neurotoxicity / 동의생리병리학회지 / 한의병리학회 18(6) : 1666~1685

[journal] 오성준 / 2001 / 大黃(Rheum undulatum L.) 뿌리의 항산화 활성물질, Piceatannol / 한국응용생명화학회지 44(3) : 208~210

[journal] 장연희 / 2008 / Effect of Eisenia Bicyclis and Its Pill on Serum Lipid Status in Rats Fed High Fat Diet / Journal of Nutrition and Health / 한국영양학회 41(1) : 5~12

[journal] 이종철 / 2009 / The effect of Elastase inhibition and Tyrosinase by Rheum undulatum Stokes extracts / 한방안이비인후피부과학회지 / 대한한방안이비인후피부과학회 22(3) : 36~46

[journal] Joong-Keun Lee / 2007 / Analgesic and Anti-Inflammatory Effect of Scutellaria baicalensis / 대한한의학회지 / 대한한의학회 28(4) : 124~135

[journal] 윤석빈 / 2011 / Effect of Scutellariae Radix Extract on the Proinflammatory Mediators in Raw 264.7 Cells Induced by LPS / 대한본초학회지 / 대한본초학회 26(2) : 75~81

[journal] 김태균 / 2008 / Anti-inflammatory Effects of Hydrogels Containing Piroxicam and Hydrolyzed Products of Scutellariae Radix on Collagen-induced Arthritis / 약 학 회 지 / 대한약학회 52(5) : 394~401

[journal] 신정민 / 2008 / Effects of Scutellaria radix Extract on Osteoblast Differentiation and Osteoclast Formation / 한국식품과학회지 / 한국식품과학회 40(6) : 674~679

[journal] 김용주 / 2000 / 黃連 열수추출물의 항산화 활성 효과 / 생명과학회지 10(3) : 241~246

[journal] 박용기 / 2006 / Inhibitory effects of Coptidis Rhizoma on the LPS-induced production of nitric oxide and TNF-α in mouse macrophage cells / 대한본초학회지 / 대한본초학회 21(2) : 165~173

[journal] 윤광로 / 2009 / Anti-inflammatory effect of Coptidis Rhizoma / 대한본초학회지 / 대한본초학회 24(3) : 79~86

[journal] Cho JY / 1999 / 지질다당류-유도 RAW264.7 세포 내 종양 괴사인자 생성에 미치는 한방의 억제효과 / Natural Product Sciences 5(1) : 12~19

[journal] 김윤희 / 2006 / The Effects of Herbal-acupuncture with Aconiti Iateralis Preparata Radix solutionon Collagen-induced arthritis / Korean Journal of Acupuncture / 경락경혈학회 23(2) : 137~154

[journal] 정선희 / 1997 / 附子藥鍼이 鎭痛 및 消炎作用에 미치는 영향 / 대한침구학회지 14(1) : 334~346

[journal] 박선민 / 2012 / Effects of Samkieumgamibang Extract on Osteoclast Differentiation and Osteoblast Function / 대한한방부인과학회지 / 대한한방부인과학회 25(2) : 23~42 / http://dx.doi.org/

[journal] 이태희 / 2006 / Effects of Coptidis Rhizoma and Aconiti Lateralis Preparata Radix on the Change of Plasma Corticosterone Level and Rectal Temperature Induced by LPS / 대한본초학회지 / 대한본초학회 21(2) : 77~85

[book] 박일규 / 2001 / 임상병리학 / 고려의학 : 71~106

[journal] Kalben DA / 1977 / Hypothesis and experimental confirmation of a new pharmacological model of osteoarthrosis / Arzneimittelforschung 27(3) : 527~531

[thesis] 이용수 / 2008 / Monosodium iodoacetate(MIA)로 유발된 관절염 흰쥐의 행동학적 조직학적 변화 / 석사 / 고려대학교 대학원

[journal] Fernihough J / 2004 / Pain related behaviour in two models of osteoarthritis in the rat knee / Pain 112(1-2) : 83~93

[journal] Zhu CZ / 2005 / Role of cetral and peripheral mGluR5 receptors in post-operative pain in rats / Pain 114 : 195~202

[journal] Sims JE / 1988 / cDNA expression cloning of the IL-1 receptor, a member of the immunoglobulin superfamily / Science 241(4865) : 585~589

[journal] Sidney LE / 2014 / Comparison of osteogenic differentiation of embryonic stem cells and primary osteoblasts, revealed by responses to IL-1β, TNF-α and IFN-γ / Stem Cells Dev 23(6) : 605~617

[journal] Hughes FJ / 1999 / Cytokine-induced prostaglandin E2synthesis and cyclooxygenase-2 activity are regulated both by a nitric oxide-dependent and-independent mechanism in rat osteoblasts in vitro / J Biol Chem 274(3) : 1776~1782

[journal] Iking-Konert C / 2014 / Interleukin-6 inhibition as a potential therapeutic target in rheumatic diseases / Z Rheumatol 73(3) : 269~276

[journal] Yao X / 2014 / Targeting interleukin-6 in inflammatory autoimmune diseases and cancers / Pharmacology and Therapeutics 141(2) : 125~139

[journal] Farina AR / 2012 / Alendronate promotes plasmin-mediated MMP-9 inactivation by exposing cryptic plasmin degradation sites within the MMP-9 catalytic domain / FEBS Letters 586(16) : 2366~2374

[journal] 김용삼 / 2012 / Expression level and glycan dynamics determine the net effects of TIMP-1 on cancer progression / BMB Reports / 생화학분자생물학회 45(11) : 623~628

[journal] Lipari L / 2013 / Expression of gelatinases(MMP-2, MMP-9)in human articular cartilage / Int J Immunopathol Pharmacol 26(3) : 817~823

[journal] Naito K / 1999 / Measurement of matrix metalloproteinases(MMPs)and tissue inhibitor of metalloproteinases-1(TIMP-1)in patients with knee osteoarthritis : comparison with generalized osteoarthritis / Rheumatology 38(6) : 510~515

[journal] Martel-Pelletier J / 2003 / Cyclooxygenase-2 and prostaglandins in articular tissues / Semin Arthritis Rheum 33(3) : 155~167

[book] Abbas A / 2013 / 세포분자면역학 / 범문에듀케이션 : 537~

[journal] Grespan R / 2008 / CXCR2-specific chemokines mediate leukotriene B4-dependent recruitment of neutrophils to inflamed joints in mice with antigen-induced arthritis / Arthritis & Rheumatism 58(7) : 2030~2040

[journal] Atik OS / 1990 / Leukotriene B4 and prostaglandin E2-like activity in synovial fluid in osteoarthritis / Prostaglandins, leukotrienes, and essential fatty acids 39(4) : 253~254

[journal] 권종원 / 2010 / Micro-CT Arthrographic Analysis of Monosodium Iodoacetate-Induced Osteoarthritis in Rat Knees / 대한영상의학회지 / 대한영상의학회 62(5) : 483~490

[journal] Király K / 1996 / Safranin O reduces loss of glycosaminoglycans from bovine articular cartilage during histological specimen preparation / Histochem J 28(2) : 99~107

KJC Korea
Journal Central

Statistics

Tools

Sharing

Issue List

Citation status