Objectives: This study was designed to investigate the effect of Gami-cheongpyesagan-tangextract (GCST) on high fat diet-induced obesity in rats.
Methods: The mice were divided into six groups; normal diet control, high fat diet control (HFD),HFD＋GCST administrated group (100, 200, and 400 mg/kg) and olistat-admistrated group.
Obesity was induced by high fat diet (45%) for 7 weeks in mice, and GCST was administratedorally every day for 7 weeks. The body weight, food intake, and serological markers such as totalcholesterol, triglyceride, lipid contents, leptin, adiponectin and glutamic oxaloacetictransaminase/glutamic pyruvic transaminase were measured in mice. The mRNA expression ofobese-associating genes such as sterol regulatory element-binding protein (SREBP)-1c, fattyacid synthase (FAS), stearoyl-CaP desaturase (SCD-1), peroxisome proliferator-activatedreceptor (PPAR)-, COA oxidase (ACO), and carnitine palmitoyltransferase (CPT-1) wasanalyzed by reverse transcription polymerase chain reaction.
Results: The administration of GCST at 400 mg/kg, significantly reduced the increase of bodyweight and food intake as well as food efficiency compared to HFD group. GCST decreased theserum levels of triglyceride, total cholesterol, low-density lipoprotein-cholesterol, leptin in HFDcontrol group and inhibited lipid accumulation in liver and adipose tissues, but did not increasehigh-density lipoprotein-cholesterol. In the liver tissues of GCST administrated HFD group, themRNA levels of SREBP-1c, FAS and SCD-1 were decreased and the mRNA levels of PPAR-,ACO, and CPT-1 were increased.
Conclusions: These results indicate that GCST could improve high fat diet induced obesitythrough inhibiting the hyperlipidemia in fatty Liver. It suggest that GCST may be used clinically fordeclining the accumultion of body fat with hyperlipidemia.