Mutations in the tyrosine kinase domain of the EGFR gene are rare in the Korean Oral Squamous Cell Carcinoma

이은주

Journal of The Korea Society of Computer and Information

Abbr : JKSCI
2016, 21(9), pp.101-106
Publisher : The Korean Society Of Computer And Information
Research Area : Computer Science

이은주 ¹

¹ 대전보건대학교

ABSTRACT

The epidermal growth factor receptor(EGFR) protein kinase signaling is an important pathway in cancer development and recently reported that EGFR and its kinase domain molecules are mutated in various of cancers including head and neck cancer. Functional deregulation of EGFR due to mutations in coding exons and copy number amplification is the most common event in cancers, especially among receptor tyrosine kinases(TK). We have analyzed Korean oral squamous cell carcinomas (OSCC) cell lines for mutations in EGFRTK. Exons encoding the hot-spot regions in the TK domain of EGFR (exons 17 to 23) were amplified by using polymerase chain reaction(PCR) and sequenced directly. EGFR expression was also analyzed in 8 OSCC cell lines using western blotting. Data analysis of the EGFR exons 17 to 23 coding sequences did not show any mutations in the 8 OSCC cell lines that were analyzed. The absence of mutations indicate that protein overexpression might be responsible for activation rather than mutation.
KEYWORDS

Receptor-Epidermal Growth Factor , protein-tyrosine kinase , carcinoma-squamous cell , Head and Neck Neoplasms , mouth neoplasms , exons
REFERENCES (21)

[journal] Luka Milas / 2003 / Chemoradiotherapy: Emerging treatment improvement strategies / Head & Neck / Wiley-Blackwell 25 (2) : 152~167 / https://doi.org/10.1002/hed.10232

[journal] C. J. Langer / 2012 / Exploring Biomarkers in Head and Neck Cancer / Cancer 118 (16) : 3882~3892

[journal] E. S. Lampri / 2015 / Biomarkers of head and neck cancer, tools or a gordian knot? / Int J Clin Exp Med 8 (7) : 10340~10357

[journal] S. P. D’Angelo / 2011 / Incidence of EGFR exon 19 deletions and L858R in tumor specimens from men and cigarette smokers with lung adenocarcinomas / J Clin Oncol 29 : 2066~2070

[journal] A. F. Gazdar / 2009 / Activating and resistance mutations of EGFR in non-small-cell lung cancer : role in clinical response to EGFR tyrosine kinase inhibitors / Oncogene 28 : 24~31

[journal] F. Ciardiello / 2001 / A novel approach in the treatment of cancer : targeting the epidermal growth factor receptor / Clin Cancer Res 7 : 2958~2970

[journal] Q. Liu / 2013 / Lack of epidermal growth factor receptor gene mutations in exons 19 and 21 in primary lymphoepithelioma-like carcinoma of the lung / Thoracic Cancer 5 : 63~67

[journal] P. Stephens / 2004 / Lung cancer : intragenic ERBB2 kinase mutations in tumors / Nature 431 : 525~526

[journal] H. Shigematsu / 2005 / Clinical and biological features associated with epidermal growth factor receptor gene mutations in lung cancers / J Natl Cancer Inst 97 : 339~462

[journal] 이은주 / 2005 / Characterization of newly established oral cancer cell lines derived fromsix squamous cell carcinoma and two mucoepidermoid carcinoma cells / Experimental and Molecular Medicine / 생화학분자생물학회 37 (5) : 379~390

[journal] 전남경 / 2014 / The GSK-3β/Cyclin D1 Pathway is Involved in the Resistance of Oral Cancer Cells to the EGFR Tyrosine Kinase Inhibitor ZD1839 / 대한의생명과학회지 / 대한의생명과학회 20 (2) : 85~95

[journal] S. Rajendran / 2013 / Lack of RING finger domain(RFD)mutations of the c-cbl gene in oral squamous cell carcinomas in Chennai, India / Asian Pac J Cancer Prev 14 : 1073~1075

[journal] E. E. Cohen / 2003 / Phase II trial of ZD1839 in recurrent or metastatic squamous cell carcinoma of the head and neck / J Clin Oncol 21 : 1980~1987

[book] P. M. Harari / 2003 / Molecular Targeting of the Epidermal Growth Factor Receptor / Lippincott, Williams and Wilkings

[journal] T. Fukui / 2007 / Gefitinib and epidermal growth factor receptor gene mutation / Gan Kagaku Ryoho 34 : 1168~1172

[journal] A. F. Gazdar / 2004 / Mutations and addiction to EGFR : the Achilles heal of lung cancers? / Trends Mol Med 10 : 481~486

[journal] E. E. Cohen / 2005 / Response of some head and neck cancers to epidermal growth factor receptor tyrosine kinase inhibitors may be linked to mutation of ERBB2 rather than EGFR / Clin Cancer Res 11 : 8105~8108

[journal] S. E. Wang / 2006 / HER2kinase domain mutation results in constitutive phosphorylation and activation of HER2 and EGFR and resistance to EGFR tyrosine kinase inhibitors / Cancer Cell 10 : 25~38

[journal] Y. Lemos-González / 2007 / Absence of activating mutations in the EGFR kinase domain in Spanish head and neck cancer patients / Tumour Biol 28 : 273~279

[journal] J. Loeffler-Ragg / 2006 / Low incidence of mutations in EGFR kinase domain in Caucasian patients with head and neck squamous cell carcinoma / Eur J Cancer 42 : 109~111

[journal] J. W. Lee / 2006 / Absence of EGFR mutation in the kinase domain in common human cancers besides non-small cell lung cancer / Int J Cancer 113 : 510~511
KCI Citation (0)

KJC Korea
Journal Central

Statistics

Tools