The purpose of this study is to investigate the occupational stress of physical therapists. A total of 302 physical therapists participated in this study. All participants were responded to a Korean Occupational Stress Scale-short form to investigate occupational stress. We compared the occupational stress of physical therapists according to the gender, employee type, medical facility, and the department. Occupational stress showed a significant difference only in gender. Overall, the physical therapist showed low occupational stress in this study. However, among the 7 items on the Korean Occupational Stress Scale-short form, the ‘Job demand’ item score was higher than other items. This study suggests that the hospital administrator needs to provide proper job demand and environment considering the level of the physical therapists in order to effective occupational stress management.