This study verified the effects that a programming-based TPACK education program, through design-based research on TPACK, had on pre-service teachers. The research subjects were pre-service teachers attending teacher training at a university in Korea, and 129 control groups and 126 experimental groups were recruited through lectures. The treatment used a TPACK educational program developed in a design-based study (Kim & Lee, 2019). A TPACK test tool developed by Park and Kang (2014) was used. The test results were analyzed via independent sample t-test and paired sample t-test. The results show that the programming-based TPACK education program (first cycle) was only effective in improving TPK and TPACK, but an effective educational program was developed to improve PCK, TCK, TK (second cycle), PK (third cycle), and CK (final cycle). This design-based research therefore confirmed the development of a programming-based TPACK education program that was effective for improving the TPACK of pre-service teachers.