This study was conducted to identify differences in Practical Performance of Nurse between Comprehensive Nursing Care Service Ward and General Service Ward. The subjects of this study were 185 nurses from S hospital, Comprehensive Nursing Care service operating hospital, located in D city. This study used the measuring tool “Nursing Professionalism Scale” developed by Yoon etc(2005) and “Practical Performance Scale” developed by Lee etc(2005). The collected data were analyzed by descriptive statistics, t-test, ANOVA, Duncan test, Pearson’s correlation coefficients and multiple regression using SPSS 20.0 version. As a result of the study, Comprehensive Nursing Care Service Ward nurses have higher Nursing Professionalism than General Service Ward nurses. Also, Nursing Professionalism has an effect on improvement of Practical Performance on Both ward nurses. Therefore, the study can be applied to improve the Practical Performance of the nurses through the establishment of the Nursing Professionalism.