Root bark extract of Cudrania tricuspidata reduces LPS-induced inflammation in macrophages of atherogenic mice

Lee Mi Ran

Journal of The Korea Society of Computer and Information

Abbr : JKSCI
2020, 25(10), pp.115-123
Publisher : The Korean Society Of Computer And Information
Research Area : Computer Science

Lee Mi Ran ¹

¹중원대학교

ABSTRACT

In this paper, we propose to evaluate the potential anti-inflammatory properties of root bark extract of Cudrania (C.) tricuspidata on lipopolysaccharide (LPS)-induced inflammation in macrophages of apolipoprotein E (ApoE) knockout (ApoE -/- ) mice, murine model of atherosclerosis. Atherosclerosis is a chronic vascular inflammatory disease. C. tricuspidata is a small tree of the Moraceae family and its extract has anti-inflammatory activities. However, its role in the progress of atherosclerosis is not yet clear. To determine anti-inflammatory effects of C. tricuspidata in atherogenesis, we applied LPS in peritoneal macrophages of ApoE -/- mice and measured cell viability by CCK-8 and expression of pro-inflammatory cytokines by qRT-PCR following treatment with root bark extract of C. tricuspidata. Research data was expressed as differences between the cells treated with LPS and root bark extract and the cells treated with LPS alone (control) by a two-tailed non-parametric Mann–Whitney U-test using GraphPad Instat program. No cytotoxic effect was observed when the cells were treated with the extract at concentrations ≤ 100 ㎍/mL. The expression of inflammatory cytokines, including MCP-1, IL-1β, IFN-γ, TNF-α, and IL-6 were inhibited by the extract. These results indicated that the extract has an anti-inflammatory effect and therefore a possible role in the treatment of atherosclerosis.
KEYWORDS

Cudrania tricuspidata, Lipopolysaccharide, Inflammation, Peritoneal macrophage, Atherosclerosis
REFERENCES (33)

[journal] Sarah Zaman / 2019 / Sudden Death Risk-Stratification in 2018–2019: The Old and the New / Heart, Lung and Circulation / Elsevier BV 28(1) : 57~64 / 10.1016/j.hlc.2018.08.027

[journal] Naghavi M / 2015 / Global, regional, and national age–sex specific all-cause and cause-specific mortality for 240 causes of death, 1990–2013: a systematic analysis for the Global Burden of Disease Study 2013 / The Lancet / Elsevier BV 385(9963) : 117~171 / 10.1016/S0140-6736(14)61682-2

[other] Statistics Korea / 2019 / Cause-of-death statistics in 2019 : 1~56

[journal] Gallino A / 2014 / Non-coronary atherosclerosis / Eur Heart J 35(17) : 1112~1119 / 10.1093/eurheartj/ehu071

[journal] Michael A. Gimbrone / 2016 / Endothelial Cell Dysfunction and the Pathobiology of Atherosclerosis / Circulation Research / Ovid Technologies (Wolters Kluwer Health) 118(4) : 620~636 / 10.1161/CIRCRESAHA.115.306301

[journal] Marchio P / 2019 / Targeting Early Atherosclerosis : A Focus on Oxidative Stress and Inflammation / Oxid Med Cell Longev 2019 : 8563845~ / 10.1155/2019/8563845

[journal] Ridker, P.M. / 2016 / Residual inflammatory risk: addressing the obverse side of the atherosclerosis prevention coin / Eur. Heart J. 37(22) : 1720~1722 / 10.1093/eurheartj/ehw024

[journal] Dimitry A. Chistiakov / 2017 / Mechanisms of foam cell formation in atherosclerosis / Journal of Molecular Medicine / Springer Science and Business Media LLC 95(11) : 1153~1165 / 10.1007/s00109-017-1575-8

[journal] Khojasteh Malekmohammad / 2019 / Antioxidants and Atherosclerosis: Mechanistic Aspects / Biomolecules / MDPI AG 9(8) : 301~ / 10.3390/biom9080301

[journal] Mihaylova B / 2012 / Cholesterol Treatment Trialists’(CTT) Collaborators. The effects of lowering LDL cholesterol with statin therapy in people at low risk of vascular disease: meta-analysis of individual data from 27 randomised trials / The Lancet / Elsevier BV 380(9841) : 581~590 / 10.1016/S0140-6736(12)60367-5

[journal] Paul M Ridker / 2008 / Rosuvastatin to Prevent Vascular Events in Men and Women with Elevated C-Reactive Protein / New England Journal of Medicine / Massachusetts Medical Society 359(21) : 2195~2207 / 10.1056/NEJMoa0807646

[journal] John R. Guyton / 2014 / An assessment by the Statin Intolerance Panel: 2014 update / Journal of Clinical Lipidology / Elsevier BV 8(3) : S72~S81 / 10.1016/j.jacl.2014.03.002

[journal] Jidong Sung / 2014 / Lipid-Lowering Treatment Practice Patterns in Korea: Comparison with the Data Obtained from the CEPHEUS Pan-Asian Study / Journal of Atherosclerosis and Thrombosis / Japan Atherosclerosis Society 21(11) : 1219~1227 / 10.5551/jat.23242

[journal] Robert M. Stoekenbroek / 2018 / PCSK9 inhibitors in clinical practice: Delivering on the promise? / Atherosclerosis / Elsevier BV 270 : 205~210 / 10.1016/j.atherosclerosis.2017.11.027

[journal] Lan-Ting Xin / 2017 / Cudrania tricuspidata: an updated review on ethnomedicine, phytochemistry and pharmacology / RSC Advances / Royal Society of Chemistry (RSC) 7(51) : 31807~31832 / 10.1039/C7RA04322H

[journal] Yang Hee Jo / 2017 / Prenylated xanthones from the roots of Cudrania tricuspidata as inhibitors of lipopolysaccharide-stimulated nitric oxide production / Archiv der Pharmazie / Wiley 350(1) : e1600263~ / 10.1002/ardp.201600263

[journal] 조삼석 / 2019 / Cudrania Tricuspidata Extract and Its Major Constituents Inhibit Oxidative Stress-Induced Liver Injury / Journal of Medicinal Food / 한국식품영양과학회 22(6) : 602~613 / https://doi.org/10.1089/jmf.2018.4322

[journal] Xinwei Jiang / 2019 / Scandenolone from Cudrania tricuspidata fruit extract suppresses the viability of breast cancer cells (MCF-7) in vitro and in vivo / Food and Chemical Toxicology / Elsevier BV 126 : 56~66 / 10.1016/j.fct.2019.02.020

[journal] Yang Hee Jo / 2019 / Xanthones from the stems of Cudrania tricuspidata and their inhibitory effects on pancreatic lipase and fat accumulation / Bioorganic Chemistry / Elsevier BV 92 : 103234~ / 10.1016/j.bioorg.2019.103234

[journal] Seung-Bin Lee / 2018 / Kaempferol 7- O - β -D-glucoside isolated from the leaves of Cudrania tricuspidata inhibits LPS-induced expression of pro-inflammatory mediators through inactivation of NF-κB, AP-1, and JAK-STAT in RAW 264.7 macrophages / Chemico-Biological Interactions / Elsevier BV 284 : 101~111 / 10.1016/j.cbi.2018.02.022

[journal] Ki Hun Park / 2006 / Anti-atherosclerotic and anti-inflammatory activities of catecholic xanthones and flavonoids isolated from Cudrania tricuspidata / Bioorganic & Medicinal Chemistry Letters / Elsevier BV 16(21) : 5580~5583 / 10.1016/j.bmcl.2006.08.032

[journal] Xusheng Li / 2020 / Bioactive compounds from Cudrania tricuspidata: A natural anticancer source / Critical Reviews in Food Science and Nutrition / Informa UK Limited 60(3) : 494~514 / 10.1080/10408398.2018.1541866

[journal] Tomoko Fujimoto / 1984 / Components of Root Bark of Cudrania tricuspidata l.1,2 Structures of Four New Isoprenylated Xanthones, Cudraxanthones A, B, C and D / Planta Medica / Georg Thieme Verlag KG 50(03) : 218~221 / 10.1055/s-2007-969682

[journal] 김옥경 / 2013 / Anti-wrinkle Activity of a Curdrania tricuspidata Extract on Ultraviolet-induced Photoaging / 한국식품영양과학회지 / 한국식품영양과학회 42(4) : 608~614

[journal] Taehun Lee / 2015 / Effects of Cudrania tricuspidata Fruit Extract and Its Active Compound, 5,7,3',4'-Tetrahydroxy-6, 8-diprenylisoflavone, on the High- Affinity IgE Receptor-Media ted Activation of Syk in Mast Cells / Journal of Agricultural and Food Chemistry / American Chemical Society (ACS) 63(22) : 5459~5467 / 10.1021/acs.jafc.5b00903

[journal] Ni Cheng / 2018 / Serum amyloid A promotes LPS clearance and suppresses LPS-induced inflammation and tissue injury / EMBO reports / EMBO 19(10) : e45517~ / 10.15252/embr.201745517

[journal] Juntang Lin / 2014 / Impact of MCP -1 in Atherosclerosis / Current Pharmaceutical Design / Bentham Science Publishers Ltd. 20(28) : 4580~4588 / 10.2174/1381612820666140522115801

[journal] Riccardo V. D'Elia / 2013 / Targeting the “Cytokine Storm” for Therapeutic Benefit / Clinical and Vaccine Immunology / American Society for Microbiology 20(3) : 319~327 / 10.1128/CVI.00636-12

[journal] Monika Bartekova / 2018 / Role of cytokines and inflammation in heart function during health and disease / Heart Failure Reviews / Springer Science and Business Media LLC 23(5) : 733~758 / 10.1007/s10741-018-9716-x

[journal] Joshua Hartman / 2014 / Inflammation and Atherosclerosis / Cardiology in Review / Ovid Technologies (Wolters Kluwer Health) 22(3) : 147~151 / 10.1097/CRD.0000000000000021

[journal] 박종길 / 2011 / The role of peroxidases in the pathogenesis of atherosclerosis / BMB Reports / 생화학분자생물학회 44(8) : 497~505

[journal] 고범석 / 2014 / Adverse drug reactions after taking the extract of Cudrania tricuspidata / Allergy Asthma & Respiratory Diseases / 대한 소아알레르기 호흡기학회 2(5) : 387~390

[journal] Chang SL / 2008 / Clinical manifestations and characteristics of patients with acute generalized exanthematous pustulosis in Asia / Acta Derm Venereol 88 : 363~365 / 10.2340/00015555-0438
KCI Citation (0)

KJC Korea
Journal Central

Statistics

Tools