The purpose of this study is to find out whether smartphone overdependence serves as a significant mediating effect in the relationship of anxiety and school life adjustment to prevent and reduce problems of anxiety and smartphone overdependence among university students and to seek alternatives to improve school life adjustment. For this purpose, a structural equation model analysis was conducted on male and female university students attending a four-year university located in Gwangju Metropolitan City to verify the mediated effect. As the result of the analysis, The mediating effects of smartphone overdependence were statistically significant and presented as a partial medium, showing the direct effect of anxiety directly affecting school life adjustment and the indirect effect of anxiety on school life adjustment by means of smartphone overdependence. Based on these findings, the significance of the study and its implications were discussed, and suggestions were put forward for further study.