This study was conducted to examine the influence of 1-1-9 ambulance crews’ empathy ability and compassion fatigue on the quality of life in the COVID-19 situation, and to provide basic data on the specific system to improve the quality of life of 1-1-9 ambulance crews. From March 10 to June 15, 2021, a survey was conducted on 250 1-1-9 ambulance crews in K-do and D-si, and frequency analysis, correlation, and multiple regression analysis were conducted using SPSS/WIN 25.0 for the collected data. As a result of multiple regression analysis, it was found that the relationship between psychological variables related to COVID-19 of 1-1-9 ambulance crews on empathy ability, compassion fatigue, and quality of life had a significant influence on each sub-factor. In this paper, we propose that a system be designed to enhance 1-1-9 ambulance crews’ well-being.