The purpose of this study is to analyze how it affects functional movement and dynamic balance applying Czech get up exercise based on the principle of motor development of child for office worker women in 30s and 40s. Through random allocation, an Czech get up exercise group(n=15) and a control group(n=14) were formed to apply exercise for 12 weeks, 3 times a week, and 60 minutes a day, and the control group maintained daily life at the same period. As a result of the study, showed that Deep squat(p<.05), Hurdle step(p<.01), In-line lunge(p<.05), Trunk stability push-up(p<.001), Rotary stability (p<.01), Total score(p<.001), were significantly in the Czech get up Group, and Dynamic Balance of Lower Extremity(p<.001), Right Dynamic Balance of Lower Extremity(p<.001) were also significantly in the Czech get up Group. In conclusion, it is thought that Czech get up exercise based on the principle of motor development of child will have a positive effect on the functional movement and dynamic balance in office women, thereby increasing work efficiency as well as healthy life.