The purpose of this study was to examine and understand the effect on the exercise flow, electroencephalogram (EEG), and the photoplethysmography (PPG) following the senior citizens’ yoga participation experience. The subjects of this study were 19 senior citizens living in City I who voluntarily agreed to participate in the study, who were classified into those who are experienced in yoga (n=9) and those who were not experienced in yoga (n=10). The yoga exercise program lasted 60 minutes. After the yoga exercise program was completed, the EEG and PPG were measured, and the exercise flow survey questionnaires were prepared. As for the data processing method, the SPSS 23.0 Version for Windows was used to conduct the independent t-test, and the following results were secured. First, there are differences in the exercise flow depending on whether they were experienced in the yoga participation, yet they were not significant. Second, there were significant differences in L-Beta, M-Beta, H-Beta, Gamma, and the brain health indicators of Brain Stress and Brain Score (p<.05), depending on the experience of the senior citizens participating in yoga. Third, there were significant differences in HRV-INDEX, parasympathetic neuronal activities, and autonomic neuronal activities (p<.05), depending on the experience of the senior citizens participating in yoga. As a result of this study, it was apparent that there were differences in the EEG and PPG depending on whether the senior citizens were experienced in the yoga participation, and such results can be expected to serve as the basic data for the research of the ways to participate in the sustainable exercise along with the intent to exercise.