This study examined the effects of a short-term intensive Mind Subtraction meditation program on adults’ blood chemistry and blood pressure. Twenty-eight adults participated in a short-term intensive mediation program for 4 days and 5 nights. In this study, the effects of 27-hours meditation training were measured. The subjects of study were healthy adults who continued meditation training for more than a year, and consisted in 9 male and 19 female, whose average age was 47.86. The collected data were analyzed using paired t-test or Wilcoxon signed rank test in SPSS 24.0. The results showed that the indicators of kidney function (blood urea nitrogen and creatinine; BUN/Crt) and total cholesterol were significantly decreased after completing the meditation program. Diastolic blood pressure was significantly decreased in the normal blood pressure group, and systolic blood pressure was decreased in the elevated blood pressure group with basal systolic blood pressure above 120mmHg. In men, the enzyme which reflects liver damage or chronic inflammation, gamma-glutamyltranspeptidase (γ-GTP) also significantly decreased. Based on these results, Mind Subtraction Meditation may have a positive effect on clinical chemistry indicators and blood pressure in adults. In the future, it is suggested that more detailed scientific studies be conducted to investigate the effects of this type of meditation on physical health and disease prevention.