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pISSN : 1975-9657

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2016, Vol.10, No.1

  • 1.

    The Evaluation of the Potential Eye Irritation of Stem Cell Cosmetics using Alternative Methods

    Han-Seul Oh | C-Yoon Kim | Ju-Hee Han and 6other persons | 2016, 10(1) | pp.1~7 | number of Cited : 0
    Abstract PDF
    The global cosmetic market in 2014 was estimated to be worth to about 250 billion USD and has continued to grow. In particular, stem-cell based cosmetics, which can be used to repair skin and protect it from aging, have become recently trendy. However, the questionable safety issue of commercial stem-cell cosmetics has become a hot issue. Cosmetics consumers may buy a product without any information about the safety of products whose ingredients may have less than 10 years of commercial history. For the safety verification of cosmetics and ingredients, the classification for eye irritation needs to be conducted essentially. The Bovine Corneal Opacity & Permeability test (BCOP) is recommended as a validated alternative method for eye irritation by the Organization for Economic Co-operation and Development (OECD). Thus, in this study, we evaluated the eye irritation potential of commercial stem-cell based cosmetics by the BCOP assay, Hens Egg Test on the Chorio-Allantoic Membrane (HET-CAM) assay, and Human Umbilical Vein Endothelial cell (HUVEC) test. Our results showed that all 8 test substances were not classified as eye irritants which could cause serious eye damage according to the BCOP assay associated with histopathology and the HET-CAM assay. But four test substances showed to be mild irritants in the HUVEC test. To the best of our knowledge, our results are the first reported data about the classification of the potential eye irritation of stem-cell based cosmetics, and demonstrate that the validated BCOP assay combined with other alternative tests such as the HET-CAM assay and the HUVEC test, may be able to indicate mild to serious eye irritants.
  • 2.

    Development of an In vitro Phototoxicity Test Using the Human Reconstituted Epidermis Model Developed in Korea

    Yong-Sun Lee | Jung-Sun Yi | Hye-Rim Lim and 7other persons | 2016, 10(1) | pp.1~9 | number of Cited : 0
    Abstract PDF
    Human reconstituted epidermis models have been widely used in evaluating phototoxicity. However, human reconstituted epidermis models made in Korea have not been employed to assess phototoxicity. In this study, we conducted an in vitro phototoxicity test with Neoderm® -E, human reconstituted epidermis model from Korea, in order to see if the model could be used for the evaluation of phototoxicity. Firstly, we performed an UVA sensitivity test and selected 6 J/cm^2 for an UVA irradiation dose. Chlorpromazine as a positive control was then tested to confirm that the substance induces phototoxicity in this model. Afterwards, 3 phototoxins and 2 non-phototoxins were tested using Neoderm® -E and Epiderm TM , a generally used phototoxicity test model, to compare prediction results for each chemical. The results indicated that all those 5 chemicals were correctly predicted with Neoderm® -E, which was accordant with the phototoxic information on them. However, only 3 chemicals were correctly predicted with Epiderm TM . Consequently, the predictive capacity of the test with Neoderm® -E could be higher than that using Epiderm TM . Overall, we reached the conclusion that Neoderm® -E could be used in evaluating phototoxicity.
  • 3.

    The Application of Good Laboratory Practice (GLP) on in Vitro Skin Irritation Test

    Won-Hee Lee | Nam-Soo Kim | Bo-ra Kim | 2016, 10(1) | pp.1~6 | number of Cited : 0
    Abstract PDF
    Various guidelines of alternative test have been published following increased interest in alternative test. Among these guidelines, in vitro skin irritation test is composed of applying a test substance on an artificial human skin model and determining skin irritation measured by cell viability assay using MTT test method. To perform this test to become objectively and reliably, we built all the test procedures following Good Laboratory Practice (GLP). This paper introduces the in vitro skin irritation test method using a human skin model and describes the process how researcher have been through to comply with GLP.
  • 4.

    Current Status of Alternatives to Animal Experiments in Advanced Foreign Countries

    Jin-Koo Lee | Eun-Ho Lee | Myeung-Ju Kim and 4other persons | 2016, 10(1) | pp.1~16 | number of Cited : 2
    Abstract PDF
    The recognition of welfare for experimental animal based on 3Rs principles has been spreading throughout the developed countries and therefore, on the demand of alternatives to animal experiments have been continually increased, especially in advanced countries. Recently, EU prohibited animal uses for developing cosmetic products at any level and some other countries applied already or are trying to setting the similar level of limitation of animal uses for cosmetic industries. According to the global trends, the marketing ban for animal used cosmetics will be applied soon in Korea. In this study, we summarize the polices, regulations, research developments, and validation processes of the animal alternatives methods of foreign countries and use those as a basal information for improving the alternatives regulation and developments. This research project was mainly focused to summarize and analyze related topics including, 1) Investigation of current policies of alternatives in EU, Japan, and USA etc. 2) Investigation of current regulatory trends of alternatives in foreign countries. 3) Investigation of current research trends of alternatives in foreign countries 4) Investigation of current validation processes of alternatives in foreign countries. Investigating the latest data on the status of animal alternatives methods and based on those, someone could use our study for setting the important research theme, roadmap for long-term development strategy of animal alternatives. However, due to the dynamic update trends in the alternative methods in many countries, the newly updated documents could be released at any time.
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