The recognition of welfare for experimental animal based on 3Rs principles has been spreading throughout the developed countries and therefore, on the demand of alternatives to animal experiments have been continually increased, especially in advanced countries. Recently, EU prohibited animal uses for developing cosmetic products at any level and some other countries applied already or are trying to setting the similar level of limitation of animal uses for cosmetic industries. According to the global trends, the marketing ban for animal used cosmetics will be applied soon in Korea. In this study, we summarize the polices, regulations, research developments, and validation processes of the animal alternatives methods of foreign countries and use those as a basal information for improving the alternatives regulation and developments. This research project was mainly focused to summarize and analyze related topics including, 1) Investigation of current policies of alternatives in EU, Japan, and USA etc. 2) Investigation of current regulatory trends of alternatives in foreign countries. 3) Investigation of current research trends of alternatives in foreign countries 4) Investigation of current validation processes of alternatives in foreign countries. Investigating the latest data on the status of animal alternatives methods and based on those, someone could use our study for setting the important research theme, roadmap for long-term development strategy of animal alternatives. However, due to the dynamic update trends in the alternative methods in many countries, the newly updated documents could be released at any time.