A number of studies have been introduced on the use of quaternary cationic agents having various reactive groups, which can improve the substantivity of anionic dyes, specially direct dyes, towards cotton. In the case of direct dyes, it is well known that they are widely used due to their convenience to apply and low cost, whilst they display poor levels of fastness properties to washing. Thus many applications using direct dyes in the textile fields have been still enjoyed in the areas where a higher level of wet fastness is specially not required.This work herein comprises that in order to improve the substantivity of direct dyes towards cotton, cellulose-reactive allylamine polymer namely, triazinyl N,N'-dimethyl-N,N'-diallyl ammonium chloride was prepared and treated onto cotton to provide cationic properties within substrates. This application showed that even low concentration of electrolytes being present, the direct dyes were exhausted well on the cationized cotton and that the rate of dye uptake by treated cotton was faster than that of untreated sample. Furthermore the antimicrobial properties were observed from the cationized samples.