The purpose of this study was to examine the effect of covering yarn’s twist on mechanical properties of knitted fabrics of composite yarns with single covering process. Four yarns that were used in this study: two different composite yarns made from the four kinds of cotton and functional polyester(Poly-A) with the ratio of 52:48, and the rest two yarns are the original cotton 100% yarn and the poly-A 100% yarn. The two kinds of composite yarns, CP1 and CP2, were processed on the single covering process. CP1 was applied on the single covering process with S-800 tpm, where Poly-A was used as covering yarn and cotton was used as core yarn. CP2 was applied on the same process as CP1 except that Poly-A had been applied on the two-for-one twisting process with S-400 tpm on the previous step.Sixteen mechanical properties of all the four knitted fabrics knitted under the same knitting conditions were measured by KES-FB system with the outer knit condition. And 3D CAD dressmaking simulations, which were driven by some of the mechanical properties, were presented. The results were as follows: CP2 had high RT values with twisting of covering yarn. CP2 also had high B, 2HB values because of higher linear density. SMD was affected rather by twisting of covering yarn than by fine hair of the cotton surface. Twisting of covering yarn made it decreasing T value and increasing W value. Dressmaking 3D CAD simulations showed that there are buckling effects on CP2 because of high bending rigidity and shear rigidity.