Effects of Pyrola japonica Extracts on Osteoclast Differentiation and Bone Resorption (녹제초 추출물이 파골세포 분화 및 골 흡수에 미치는 영향)

JungSik-Park (박정식); Lim Hyung Ho (임형호)

doi:10.18325/jkmr.2019.29.2.135

Effects of Pyrola japonica Extracts on Osteoclast Differentiation and Bone Resorption

Journal of Korean Medicine Rehabilitation
Abbr : JKMR
2019, 29(2), pp.135-147
DOI : 10.18325/jkmr.2019.29.2.135
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Medicine and Pharmacy > Korean Medicine
Received : March 28, 2019
Accepted : April 12, 2019
Published : April 30, 2019

JungSik-Park ¹, Lim Hyung Ho ¹

¹가천대학교 한의과대학

Accredited

ABSTRACT

Objectives This study was performed to evaluate the effect of Pyrola japonica extract (NJ) and its principal constituent, homoarbutin (HA) on osteoclast differentiation and gene expression and bone resorption. The osteoclastogenesis and gene expression were determined in receptor activator of nuclear factor kappa B ligand (RANKL)-stimulated RAW264.7 cell. Methods In order to evaluate the effect of HA extracted from NJ on bone resorption, osteoclasts were used to be differentiated and formed by stimulating RAW264.7 cells with RANKL. Tartarate-resistant acid phosphatase (TRAP) (+) polynuclear osteoclast formation ability was evaluated, and differentiation control genes including cathepsin K, matrix metalloproteinases-9 (MMP-9), and TRAP in osteoclast differentiation were analyzed by real-time polymerase chain reaction (PCR). Immunoblotting was performed to measure the effect of mitogen-activated protein kinase (MAPK) factors on bone resorption, and the effect of osteoclasts on osteoclast differentiation was measured. Results Both NJ and high concentration of HA blocked RANKL-stimulated differentiation from RAW264.7 cell to TRAP-positive multinucleated cells. NJ reduced RANKL-induced expression of TRAP, cathepsin K. Both NJ and high concentration of HA inhibited RANKL- mediated expression of MMP-9, nuclear factor of activated T-cells, cytoplasmic 1, and cellular Jun-fos. NJ suppressed RANKL-stimulated expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase, tumor necrosis factor-alpha, and levels of interleukins. Both NJ and HA decreased bone resorption in osteoclast-induced bone pit formation model. Conclusions These results suggest that NJ and HA blocked bone resorption by decreasing RANKL-mediated osteoclastogenesis through down-regulation of genes for osteoclast differentiation. (J Korean Med Rehabil 2019;29(2):135-147)

KEYWORDS

Osteoclast, Poncirin, Receptor activator of nuclear factor kappa B ligand (RANKL), Tartarate-resistant acid phosphatase (TRAP), Japonica extract, Homoarbutin

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Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6

Effects of Pyrola japonica Extracts on Osteoclast Differentiation and Bone Resorption

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* References for papers published after 2023 are currently being built.

Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6

Effects of Pyrola japonica Extracts on Osteoclast Differentiation and Bone Resorption

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