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@article{ART002555882},
author={Soo-Hwan Lee and Kira Parichuk and Cha Yun Yeop},
title={Affirmative Effect of Hwaweo-jeon (Huayu-jian) in Osteoblast Cells and Tibia Fracture-induced Mice},
journal={Journal of Korean Medicine Rehabilitation},
issn={1229-1854},
year={2020},
volume={30},
number={1},
pages={13-29},
doi={10.18325/jkmr.2020.30.1.13}

TY - JOUR
AU - Soo-Hwan Lee
AU - Kira Parichuk
AU - Cha Yun Yeop
TI - Affirmative Effect of Hwaweo-jeon (Huayu-jian) in Osteoblast Cells and Tibia Fracture-induced Mice
JO - Journal of Korean Medicine Rehabilitation
PY - 2020
VL - 30
IS - 1
PB - The Korean Academy Of Oriental Rehabilitation Medicine
SP - 13
EP - 29
SN - 1229-1854
AB - Objectives This study was performed to decide the bone union effect of Hwaweo-jeon on tibia fractured mice. Methods In this study, laboratory experiments were implemented by the stage of in vitro and in vivo. In in vitro, MC3T3-E1 cells were treated with various concentration of Hwaweo-jeon extract (HWJ). To investigate effect of HWJ for osteoblast, relative mRNA expression of 5 substances (alkaline phosphatase [ALP], runt-related transcription factor 2 [Runx2], osteocalcin [OCN], osterix [OSX] and collagen type Ⅱ alpha 1 chain [Col2a1]) was used as a marker of osteogenesis. In order to determine HWJ’s effect for fracture healing, relative gene expression level of ALP, Runx2, OCN, OSX and Col2a1 were used to find out the influence to osteoblast. Furthermore, receptor activator of nuclear factor kappa-B ligand and osteoprotegerin relative mRNA expression were used to estimate the impact to osteoclast. Also, X-ray was used for the purpose of identifying bone union in tibia-fracture mouse model. Results In in vitro experiment, most part of relative mRNA expression were increased compared to control group. In in vivo and in vitro experiment, HWJ induced osteoblast activitation by verifying relative mRNA expression of 5 substances. And in vivo experiment, we can also identify that HWJ triggered osteoclast activation during early stage of tibia fracture. Furthermore, X-ray pictures show noticeable recovery of tibia fracture. Conclusions HWJ extract promotes bone union by facilitating the osteoblast. But, HWJ may occur liver & kidney toxicity over specific concentration. Therefore, when HWJ is applied to human body, doctors have to follow up the liver function test & renal function test of patient. (J Korean Med Rehabil 2020; 30(1):13-29)
KW - Hwaweo-jeon (Huayu-jian);Tibia fractured mice;Osteoblasts;Osteoclasts;Bone union;mRNA expression
DO - 10.18325/jkmr.2020.30.1.13
ER -

Soo-Hwan Lee, Kira Parichuk and Cha Yun Yeop. (2020). Affirmative Effect of Hwaweo-jeon (Huayu-jian) in Osteoblast Cells and Tibia Fracture-induced Mice. Journal of Korean Medicine Rehabilitation, 30(1), 13-29.

Soo-Hwan Lee, Kira Parichuk and Cha Yun Yeop. 2020, "Affirmative Effect of Hwaweo-jeon (Huayu-jian) in Osteoblast Cells and Tibia Fracture-induced Mice", Journal of Korean Medicine Rehabilitation, vol.30, no.1 pp.13-29. Available from: doi:10.18325/jkmr.2020.30.1.13

Soo-Hwan Lee, Kira Parichuk, Cha Yun Yeop "Affirmative Effect of Hwaweo-jeon (Huayu-jian) in Osteoblast Cells and Tibia Fracture-induced Mice" Journal of Korean Medicine Rehabilitation 30.1 pp.13-29 (2020) : 13.

Soo-Hwan Lee, Kira Parichuk, Cha Yun Yeop. Affirmative Effect of Hwaweo-jeon (Huayu-jian) in Osteoblast Cells and Tibia Fracture-induced Mice. 2020; 30(1), 13-29. Available from: doi:10.18325/jkmr.2020.30.1.13

Soo-Hwan Lee, Kira Parichuk and Cha Yun Yeop. "Affirmative Effect of Hwaweo-jeon (Huayu-jian) in Osteoblast Cells and Tibia Fracture-induced Mice" Journal of Korean Medicine Rehabilitation 30, no.1 (2020) : 13-29.doi: 10.18325/jkmr.2020.30.1.13

Soo-Hwan Lee; Kira Parichuk; Cha Yun Yeop. Affirmative Effect of Hwaweo-jeon (Huayu-jian) in Osteoblast Cells and Tibia Fracture-induced Mice. Journal of Korean Medicine Rehabilitation, 30(1), 13-29. doi: 10.18325/jkmr.2020.30.1.13

Soo-Hwan Lee; Kira Parichuk; Cha Yun Yeop. Affirmative Effect of Hwaweo-jeon (Huayu-jian) in Osteoblast Cells and Tibia Fracture-induced Mice. Journal of Korean Medicine Rehabilitation. 2020; 30(1) 13-29. doi: 10.18325/jkmr.2020.30.1.13

Soo-Hwan Lee, Kira Parichuk, Cha Yun Yeop. Affirmative Effect of Hwaweo-jeon (Huayu-jian) in Osteoblast Cells and Tibia Fracture-induced Mice. 2020; 30(1), 13-29. Available from: doi:10.18325/jkmr.2020.30.1.13

Soo-Hwan Lee, Kira Parichuk and Cha Yun Yeop. "Affirmative Effect of Hwaweo-jeon (Huayu-jian) in Osteoblast Cells and Tibia Fracture-induced Mice" Journal of Korean Medicine Rehabilitation 30, no.1 (2020) : 13-29.doi: 10.18325/jkmr.2020.30.1.13