@article{ART002580679},
author={Dae-Yeon Lee and Ju-hwi Jo and Wan-su Kim and Ho-Sung Lee and Young-woo Yi and Sang-In Park and Keon-Sang An and Lee In Hee},
title={Antioxidant Activity and Standardization of Extraction Solvents of SJ004},
journal={Journal of Korean Medicine Rehabilitation},
issn={1229-1854},
year={2020},
volume={30},
number={2},
pages={67-75},
doi={10.18325/jkmr.2020.30.2.67}
TY - JOUR
AU - Dae-Yeon Lee
AU - Ju-hwi Jo
AU - Wan-su Kim
AU - Ho-Sung Lee
AU - Young-woo Yi
AU - Sang-In Park
AU - Keon-Sang An
AU - Lee In Hee
TI - Antioxidant Activity and Standardization of Extraction Solvents of SJ004
JO - Journal of Korean Medicine Rehabilitation
PY - 2020
VL - 30
IS - 2
PB - The Korean Academy Of Oriental Rehabilitation Medicine
SP - 67
EP - 75
SN - 1229-1854
AB - Objectives SJ004 is a natural herbal medicine that contains Acyranthes japonica Nakai and Eucommia ulmoides Oliver traditionally used for joint and spinal diseases. This study aimed to establish an efficient method of extracting SJ004 to standardize using the yield, high-performance liquid chromatography (HPLC), and antioxidant assay.
Methods SJ004 was extracted with distilled water, 70% and 100% of ethyl alcohol (EtOH). The method validation of 20-hydroxyecdysone and pinoresinol diglucoside was determined by HPLC-photo diode array and the content of SJ004 was calculated. The antioxidant activity of each extract was compared and measured using total flavonoids, total phenolic compounds, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and ferric reducing antioxidant power according to the standard protocol.
Results The yield was highest in pure water extract and lowest in 100% EtOH. But, the content of marker compounds indicating 20-hydroxyecdysone and pinoresinol diglucoside was highest in 100% EtOH extract. In the physiological activity measurement using antioxidant activity, 100% ethanol extract was highest. The limit of detection indicating 20-hydroxyecdysone and pinoresinol diglucoside were analyzed 0.33 μg/mL, 0.1616 μg/mL, and the limit of quantification were analyzed 1.01 μg/mL and 0.49 μg/mL respectively.
Conclusions The experimental results showed that the extraction conditions have a significant effect on content of marker compounds and antioxidant activity. As a result of method validation, SJ004 was standardized by 20-hydroxyecdysone and pinoresinol diglucoside. (J Korean Med Rehabil 2020;30(2):67-75)
KW - SJ004;Antioxidants;High pressure liquid chromatography;Ecdysterone
DO - 10.18325/jkmr.2020.30.2.67
ER -
Dae-Yeon Lee, Ju-hwi Jo, Wan-su Kim, Ho-Sung Lee, Young-woo Yi, Sang-In Park, Keon-Sang An and Lee In Hee. (2020). Antioxidant Activity and Standardization of Extraction Solvents of SJ004. Journal of Korean Medicine Rehabilitation, 30(2), 67-75.
Dae-Yeon Lee, Ju-hwi Jo, Wan-su Kim, Ho-Sung Lee, Young-woo Yi, Sang-In Park, Keon-Sang An and Lee In Hee. 2020, "Antioxidant Activity and Standardization of Extraction Solvents of SJ004", Journal of Korean Medicine Rehabilitation, vol.30, no.2 pp.67-75. Available from: doi:10.18325/jkmr.2020.30.2.67
Dae-Yeon Lee, Ju-hwi Jo, Wan-su Kim, Ho-Sung Lee, Young-woo Yi, Sang-In Park, Keon-Sang An, Lee In Hee "Antioxidant Activity and Standardization of Extraction Solvents of SJ004" Journal of Korean Medicine Rehabilitation 30.2 pp.67-75 (2020) : 67.
Dae-Yeon Lee, Ju-hwi Jo, Wan-su Kim, Ho-Sung Lee, Young-woo Yi, Sang-In Park, Keon-Sang An, Lee In Hee. Antioxidant Activity and Standardization of Extraction Solvents of SJ004. 2020; 30(2), 67-75. Available from: doi:10.18325/jkmr.2020.30.2.67
Dae-Yeon Lee, Ju-hwi Jo, Wan-su Kim, Ho-Sung Lee, Young-woo Yi, Sang-In Park, Keon-Sang An and Lee In Hee. "Antioxidant Activity and Standardization of Extraction Solvents of SJ004" Journal of Korean Medicine Rehabilitation 30, no.2 (2020) : 67-75.doi: 10.18325/jkmr.2020.30.2.67
Dae-Yeon Lee; Ju-hwi Jo; Wan-su Kim; Ho-Sung Lee; Young-woo Yi; Sang-In Park; Keon-Sang An; Lee In Hee. Antioxidant Activity and Standardization of Extraction Solvents of SJ004. Journal of Korean Medicine Rehabilitation, 30(2), 67-75. doi: 10.18325/jkmr.2020.30.2.67
Dae-Yeon Lee; Ju-hwi Jo; Wan-su Kim; Ho-Sung Lee; Young-woo Yi; Sang-In Park; Keon-Sang An; Lee In Hee. Antioxidant Activity and Standardization of Extraction Solvents of SJ004. Journal of Korean Medicine Rehabilitation. 2020; 30(2) 67-75. doi: 10.18325/jkmr.2020.30.2.67
Dae-Yeon Lee, Ju-hwi Jo, Wan-su Kim, Ho-Sung Lee, Young-woo Yi, Sang-In Park, Keon-Sang An, Lee In Hee. Antioxidant Activity and Standardization of Extraction Solvents of SJ004. 2020; 30(2), 67-75. Available from: doi:10.18325/jkmr.2020.30.2.67
Dae-Yeon Lee, Ju-hwi Jo, Wan-su Kim, Ho-Sung Lee, Young-woo Yi, Sang-In Park, Keon-Sang An and Lee In Hee. "Antioxidant Activity and Standardization of Extraction Solvents of SJ004" Journal of Korean Medicine Rehabilitation 30, no.2 (2020) : 67-75.doi: 10.18325/jkmr.2020.30.2.67
