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Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven

  • Textile Coloration and Finishing
  • Abbr : Text. Color. and Finish.
  • 2018, 30(4), pp.264-274
  • DOI : 10.5764/TCF.2018.30.4.264
  • Publisher : The Korean Society Of Dyers And Finishers
  • Research Area : Engineering > Fiber Engineering > Dyeing Engineering
  • Received : November 27, 2018
  • Accepted : December 11, 2018
  • Published : December 27, 2018

Lee, So Hee 1

1경상대학교 의류학과

Accredited

ABSTRACT

Immobilization of lysozyme on chitosan non-woven using glutaraldehyde(GA) was investigated. For this, 100 % chitosan non-woven was prepared as novel support for the enzyme immobilization. In addition, free lysozyme activity was examined depending on various pH and temperature by measuring time. Moreover, the optimum immobilization conditions depending on various pH, temperature, immobilization time and lysozyme concentration was evaluated. In addition, thermal stability and storage stability of immobilized lysozyme were measured. The characteristics of immobilized lysozyme was examined by FT-IR, surface morphology, and MTT assay. The results are follows: the optimal immobilization of lysozyme were pH 7.0, 25 ℃, lysozyme concentration 1.5 mg/ml, immobilization time 240 min. The immobilized lysozyme showed higher thermal stability than the free trypsin. The immobilized lysozyme activity was retained 80 % of its initial activity at 4 ℃ over 30 days of storage. The lysozyme was immobilized effectively on chitosan non-woven by observation of surface morphology.

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This paper was written with support from the National Research Foundation of Korea.