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@article{ART002421373},
author={Lee, So Hee},
title={Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven},
journal={Textile Coloration and Finishing },
issn={1229-0033},
year={2018},
volume={30},
number={4},
pages={264-274},
doi={10.5764/TCF.2018.30.4.264}

TY - JOUR
AU - Lee, So Hee
TI - Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven
JO - Textile Coloration and Finishing
PY - 2018
VL - 30
IS - 4
PB - The Korean Society Of Dyers And Finishers
SP - 264
EP - 274
SN - 1229-0033
AB - Immobilization of lysozyme on chitosan non-woven using glutaraldehyde(GA) was investigated. For this, 100 % chitosan non-woven was prepared as novel support for the enzyme immobilization. In addition, free lysozyme activity was examined depending on various pH and temperature by measuring time. Moreover, the optimum immobilization conditions depending on various pH, temperature, immobilization time and lysozyme concentration was evaluated. In addition, thermal stability and storage stability of immobilized lysozyme were measured. The characteristics of immobilized lysozyme was examined by FT-IR, surface morphology, and MTT assay. The results are follows: the optimal immobilization of lysozyme were pH 7.0, 25 ℃, lysozyme concentration 1.5 mg/ml, immobilization time 240 min. The immobilized lysozyme showed higher thermal stability than the free trypsin. The immobilized lysozyme activity was retained 80 % of its initial activity at 4 ℃ over 30 days of storage. The lysozyme was immobilized effectively on chitosan non-woven by observation of surface morphology.
KW - immobilization;chitosan;non-woven;lysozyme;glutaraldehyde
DO - 10.5764/TCF.2018.30.4.264
ER -

Lee, So Hee. (2018). Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven. Textile Coloration and Finishing , 30(4), 264-274.

Lee, So Hee. 2018, "Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven", Textile Coloration and Finishing , vol.30, no.4 pp.264-274. Available from: doi:10.5764/TCF.2018.30.4.264

Lee, So Hee "Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven" Textile Coloration and Finishing 30.4 pp.264-274 (2018) : 264.

Lee, So Hee. Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven. 2018; 30(4), 264-274. Available from: doi:10.5764/TCF.2018.30.4.264

Lee, So Hee. "Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven" Textile Coloration and Finishing 30, no.4 (2018) : 264-274.doi: 10.5764/TCF.2018.30.4.264

Lee, So Hee. Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven. Textile Coloration and Finishing , 30(4), 264-274. doi: 10.5764/TCF.2018.30.4.264

Lee, So Hee. Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven. Textile Coloration and Finishing . 2018; 30(4) 264-274. doi: 10.5764/TCF.2018.30.4.264

Lee, So Hee. Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven. 2018; 30(4), 264-274. Available from: doi:10.5764/TCF.2018.30.4.264

Lee, So Hee. "Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven" Textile Coloration and Finishing 30, no.4 (2018) : 264-274.doi: 10.5764/TCF.2018.30.4.264