@article{ART002052365},
author={Jong-Soo Kim},
title={Characterization of Differentiation of the Supernumerary Dental Pulp Stem Cells toward the Odontoblast by Application Period of Additives},
journal={Journal of the Korean academy of pediatric dentistry},
issn={1226-8496},
year={2015},
volume={42},
number={4},
pages={312-318},
doi={10.5933/JKAPD.2015.42.4.312}
TY - JOUR
AU - Jong-Soo Kim
TI - Characterization of Differentiation of the Supernumerary Dental Pulp Stem Cells toward the Odontoblast by Application Period of Additives
JO - Journal of the Korean academy of pediatric dentistry
PY - 2015
VL - 42
IS - 4
PB - The Korean Academy Of Pediatric Dentistry
SP - 312
EP - 318
SN - 1226-8496
AB - The aim of this study was to investigate the possibility of the supernumerary teeth for the stem cell source in dentistry. The Real Time Quantitative Reverse Transcription Polymerase Chain Reaction (Real Time qRT-PCR) method was used to evaluate the differentiation toward the odontoblast of the supernumerary dental pulp stem cells (sDPSCs).
Supernumerary dental pulp stem cells were obtained from 3 children (2 males and 1 female, age 7 to 9) diagnosed that the eruption of permanent teeth was disturbed by supernumerary teeth.
The common genes for odontoblasts are alkaline phosphatase (ALP), osteocalcin (OC), osteonectin (ON), dentin matrix acidic phosphoprotein 1 (DMP-1), dentin sialophosphoprotein (DSPP). The sDPSCs were treated for 0 days, 8 days and 14 days with additives and then Real Time qRT-PCR was performed in intervals of 0 days, 8 days and 14 days.
The alizarin-red solution staining was performed to visualize the stained color for the degree of calcification at 7 days, 14 days, 21 days and 28 days after treating additives to the sDPSCs.
From the result of the Real Time qRT-PCR, the manifestation exhibit maximum value at 8 days after additive treatment and shifted to a decrease trend at 14 days.
Alizarin-red solution staining exhibit light results at 7 days after staining and generalized dark result at 14 days.
Consequently, in studies with sDPSCs, appropriate treatment time of additives for Real Time qRT-PCR is 8 days. Also, a suitable period of Alizarin-red solution staining is 14 days.
KW - Odontoblast;Supernumerary Tooth;Additives;Stem Cell
DO - 10.5933/JKAPD.2015.42.4.312
ER -
Jong-Soo Kim. (2015). Characterization of Differentiation of the Supernumerary Dental Pulp Stem Cells toward the Odontoblast by Application Period of Additives. Journal of the Korean academy of pediatric dentistry, 42(4), 312-318.
Jong-Soo Kim. 2015, "Characterization of Differentiation of the Supernumerary Dental Pulp Stem Cells toward the Odontoblast by Application Period of Additives", Journal of the Korean academy of pediatric dentistry, vol.42, no.4 pp.312-318. Available from: doi:10.5933/JKAPD.2015.42.4.312
Jong-Soo Kim "Characterization of Differentiation of the Supernumerary Dental Pulp Stem Cells toward the Odontoblast by Application Period of Additives" Journal of the Korean academy of pediatric dentistry 42.4 pp.312-318 (2015) : 312.
Jong-Soo Kim. Characterization of Differentiation of the Supernumerary Dental Pulp Stem Cells toward the Odontoblast by Application Period of Additives. 2015; 42(4), 312-318. Available from: doi:10.5933/JKAPD.2015.42.4.312
Jong-Soo Kim. "Characterization of Differentiation of the Supernumerary Dental Pulp Stem Cells toward the Odontoblast by Application Period of Additives" Journal of the Korean academy of pediatric dentistry 42, no.4 (2015) : 312-318.doi: 10.5933/JKAPD.2015.42.4.312
Jong-Soo Kim. Characterization of Differentiation of the Supernumerary Dental Pulp Stem Cells toward the Odontoblast by Application Period of Additives. Journal of the Korean academy of pediatric dentistry, 42(4), 312-318. doi: 10.5933/JKAPD.2015.42.4.312
Jong-Soo Kim. Characterization of Differentiation of the Supernumerary Dental Pulp Stem Cells toward the Odontoblast by Application Period of Additives. Journal of the Korean academy of pediatric dentistry. 2015; 42(4) 312-318. doi: 10.5933/JKAPD.2015.42.4.312
Jong-Soo Kim. Characterization of Differentiation of the Supernumerary Dental Pulp Stem Cells toward the Odontoblast by Application Period of Additives. 2015; 42(4), 312-318. Available from: doi:10.5933/JKAPD.2015.42.4.312
Jong-Soo Kim. "Characterization of Differentiation of the Supernumerary Dental Pulp Stem Cells toward the Odontoblast by Application Period of Additives" Journal of the Korean academy of pediatric dentistry 42, no.4 (2015) : 312-318.doi: 10.5933/JKAPD.2015.42.4.312