Comparison of Gene Expression from Supernumerary Dental Pulp and Periodontal Ligament Stem Cells (과잉치 치수 세포와 치주인대 세포의 유전자 발현 비교)

Sangeun Lee (이상은); Kim Jongbin (김종빈); Jong-Soo Kim (김종수)

doi:10.5933/JKAPD.2018.45.2.242

Comparison of Gene Expression from Supernumerary Dental Pulp and Periodontal Ligament Stem Cells

Journal of the Korean academy of pediatric dentistry
Abbr : J Korean Acad Pediatr Dent
2018, 45(2), pp.242-249
DOI : 10.5933/JKAPD.2018.45.2.242
Publisher : The Korean Academy Of Pediatric Dentistry
Research Area : Medicine and Pharmacy > Dentistry
Received : January 16, 2018
Accepted : March 13, 2018
Published : May 31, 2018

Sangeun Lee ¹, Kim Jongbin ², Jong-Soo Kim ²

¹단국대학교 치과대학 소아치과학교실
²단국대학교

Accredited

ABSTRACT

The purpose of this study is to compare the properties of dental pulp and periodontal ligament stem cells from extracted supernumerary teeth by quantitative real-time PCR. Impacted supernumerary teeth in the maxillary anterior region were extracted. Dental pulp and periodontal ligament cells were collected from extracted supernumerary teeth on the same day. After isolation and culture of cells, compare characterization of them by using qRT-PCR. Primer sequences for odontoblasts are ONT, ALP, OCN, DMP-1 and DSPP. On dental pulp group, ONT has the largest quantity of gene expression, followed by OCN, ALP, DMP-1 and DSPP. On periodontal ligament group, ONT has the largest quantity of gene expression, followed by OCN, ALP, DSPP and DMP- 1. Analysis of quantitative gene expression data using relative quantification showed that the expression of all genes decreased in periodontal ligament cells. Dental pulp and periodontal ligament stem cells from supernumerary teeth have the properties of odontoblasts. Considering that properties, supernumerary teeth were considered a useful donor site of dental pulp and periodontal ligament stem cells.

KEYWORDS

Supernumerary dental pulp stem cells, Supernumerary periodontal ligament stem cells, Odontoblasts

Citation status

* References for papers published after 2023 are currently being built.

[journal] Irma Thesleff / 1996 / Tooth morphogenesis and cell differentiation / Current Opinion in Cell Biology 8 : 844~850

[journal] Ruch JV / 1985 / Odontoblast Differentiation and the Formation of the Odontoblast Layer / J Dent Res 64 : 489~498

[journal] Couve E / 2013 / The Amazing Odontoblast: Activity, Autophagy, and Aging / J Dent Res 92 : 765~772

[journal] Sasaki T / 1996 / Structure and Organization of Odontoblasts / Anat Rec 245 : 235~249

[journal] Papagerakis P / 2002 / Investigation of Osteocalcin, Osteonectin, and Dentin Sialophosphoprotein in Developing Human Teeth / Bone 30 : 377~385

[journal] Asaumi JI / 2004 / Radiographic examination of mesiodens and their associated complications / Dentomaxillofac Radiol 33 : 125~127

[journal] Omer RS / 2010 / Determination of the optimum time for surgical removal of unerupted anterior supernumerary teeth / Pediatr Dent 32 : 14~20

[journal] Huang GT / 2009 / Mesenchymal Stem Cells Derived from Dental Tissues vs. Those from Other Sources: Their Biology and Role in Regenerative Medicine / J Dent Res 88 : 792~806

[journal] Ulmer FL / 2010 / Stem Cells - Prospects in Dentistry / Schweiz Monatsschr Zahnmed 120 : 860~883

[journal] Gronthos S / 2002 / Stem Cell Properties of Human Dental Pulp Stem Cells / J Dent Res 81 : 531~535

[journal] Seo BM / 2004 / Investigation of multipotent postnatal stem cells from human periodontal ligament / Lancet 364 : 149~155

[journal] 송제선 / 2010 / Characterization of Stem Cells Obtained from the Dental Pulp and Periodontal Ligament of Deciduous Teeth / 조직공학과 재생의학 / 한국조직공학과 재생의학회 7(5) : 575~582

[journal] Livak KJ / 2001 / Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the <TEX>$2-{\Delta}{\Delta}CT$</TEX> Method / Methods 25 : 402~408

[journal] Gadkar VJ / 2014 / New Developments in Quantitative Real-time Polymerase Chain Reaction Technology / Curr Issues Mol Biol 16 : 1~6

[journal] Shih WY / 2016 / Clinical evaluation of the timing of mesiodens removal / J Chin Med Assoc 79 : 345~350

[journal] Russell KA / 2003 / Mesiodens - Diagnosis and Management of a Common Supernumerary Tooth / J Can Dent Assoc 69 : 362~366

[journal] Arana-Chavez VE / 2004 / Odontoblasts: the cells forming and maintaining dentine / Int J Biochem Cell Biol 36 : 1367~1373

[journal] Couve E / 1986 / Ultrastructural changes during the life cycle of human odontoblasts / Arch Oral Biol 31 : 643~651

[journal] Goldberg M / 2011 / Structure, Composition and Mineralization / Front Biosci 3 : 711~735

[journal] Begue-Kirn C / 1994 / Comparative analysis of TGFBs, BMPs, IGF1, msxs, fibronectin, osteonectin and bone sialoprotein gene expression during normal and in vitro-induced odontoblast differentiation / Int J Dev Biol 38 : 405~420

[journal] Termine JD / 1981 / Osteonectin, A Bone-Specific Protein Linking Mineral to Collagen / Cell 26 : 99~105

[journal] Abd-Elmeguid A / 2013 / Osteocalcin Expression in Pulp Inflammation / J Endod 39 : 865~872

[journal] Beertsen W / 1991 / Alkaline Phosphatase Induces the Deposition of Calcified Layers in Relation to Dentin: An in vitro Study to Mimic the Formation of Afibrillar Acellular Cementum / J Dens Res 70 : 176~181

[journal] Shiba H / 2003 / Proliferative Ability and Alkaline Phosphatase Activity with In Vivo Cellular Aging in Human Pulp Cells / J Endod 29 : 9~11

[journal] 박지현 / 2011 / Characteristics of Stem Cells Derived from the Periodontal Ligament of Supernumerary Teeth / 조직공학과 재생의학 / 한국조직공학과 재생의학회 8(4) : 123~131

[journal] Jacob A / 2014 / Transcriptional regulation of dentin matrix protein 1 (DMP1) in odontoblasts and osteoblasts / Connect Tissue Res 55 : 107~112

[journal] Alessandra T / 2011 / Distribution of DMP-1 and DSPP in human carious dentin: an immunohistochemical analysis / Italian J Anat Embryol 116 : 192~

[journal] Suzuki S / 2012 / Dentin sialophosphoprotein and dentin matrix protein-1: Two highly phosphorylated proteins in mineralized tissues / Arc Oral Biol 57 : 1165~1175

[journal] Kamata N / 2004 / Immortalization of human dental papilla, dental pulp, periodontal ligament cells and gingival fibroblasts by telomerase reverse transcriptase / J Oral Pathol Med 33 : 417~423

[journal] 소현 / 2009 / THE COMPARISON OF GENE EXPRESSION FROM HUMAN DENTAL PULP CELLS AND PERIODONTAL LIGAMENT CELLS / Restorative Dentistry and Endodontics / 대한치과보존학회 34(5) : 430~441

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Journal of the Korean academy of pediatric dentistry 2023 KCI Impact Factor : 0.48

Comparison of Gene Expression from Supernumerary Dental Pulp and Periodontal Ligament Stem Cells

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* References for papers published after 2023 are currently being built.

Journal of the Korean academy of pediatric dentistry 2023 KCI Impact Factor : 0.48

Comparison of Gene Expression from Supernumerary Dental Pulp and Periodontal Ligament Stem Cells

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