Gene Expression of Supernumerary Dental Pulp Related to the Subculture Speed: A Pilot Study (계대 배양 속도가 다른 과잉치 치수유래 줄기세포 간 유전자 발현 특성)

Yookyung Lee (이유경); Jong-Soo Kim (김종수); JISUN SHIN (신지선); Kim Jongbin (김종빈)

doi:10.5933/JKAPD.2019.46.2.219

Gene Expression of Supernumerary Dental Pulp Related to the Subculture Speed: A Pilot Study

Journal of the Korean academy of pediatric dentistry
Abbr : J Korean Acad Pediatr Dent
2019, 46(2), pp.219-225
DOI : 10.5933/JKAPD.2019.46.2.219
Publisher : The Korean Academy Of Pediatric Dentistry
Research Area : Medicine and Pharmacy > Dentistry
Received : October 13, 2018
Accepted : November 28, 2018
Published : May 30, 2019

Yookyung Lee ¹, Jong-Soo Kim ², JISUN SHIN ², Kim Jongbin ²

¹단국대학교 치과대학 소아치과학교실
²단국대학교

Accredited

ABSTRACT

The purpose of this study was to investigate the odontoblast gene expression related to the subculture speed of supernumerary dental pulp stem cells (sDPSCs). The stem cell is undifferentiated cells which has the ability to differentiate into various cells. Specific stimulation or environment induces cell differentiation, and these differentiation leads to bone or muscle formation. 20 sDPSCs were obtained from 20 children under aseptic condition. During the culture through the 10th passage, the third passage cells which showed short subculture period and 10th passage cells which showed long subculture period were earned. Each cell was divided into differentiated group and non-differentiated group. Quantitative real-time polychain reaction (q-RT-PCR) was performed for each group. The genes related to odontoblast differentiation, Alkaline Phosphatase (ALP), Osteocalcin (OCN), Osteonectin (ONT), Dentin sialophosphoprotein (DSPP) and Dentin matrix acidic phosphoprotein 1 (DMP-1), were measured. Differentiated cells showed more gene expression levels. Undifferentiated cells showed higher gene expression level in 10th passages but differentiated cells showed higher gene expression level in 3rd passages. Cells that showed faster subculture period showed relatively lower gene expression level except for OCN and DSPP.

KEYWORDS

Supernumerary Dental Pulp Stem Cells, Odontoblasts

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* References for papers published after 2023 are currently being built.

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Journal of the Korean academy of pediatric dentistry 2023 KCI Impact Factor : 0.48

Gene Expression of Supernumerary Dental Pulp Related to the Subculture Speed: A Pilot Study

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* References for papers published after 2023 are currently being built.

Journal of the Korean academy of pediatric dentistry 2023 KCI Impact Factor : 0.48

Gene Expression of Supernumerary Dental Pulp Related to the Subculture Speed: A Pilot Study

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