Application of Teeth Whitening LED for Prevention of Dental Caries : Antimicrobial Photodynamic Therapy Approach

Choa Park (박초아); Howon Park (박호원); LEE JU HYUN (이주현); Hyunwoo Seo (서현우); Lee, Si Young (이시영)

doi:10.5933/JKAPD.2020.47.1.70

Application of Teeth Whitening LED for Prevention of Dental Caries : Antimicrobial Photodynamic Therapy Approach

Journal of the Korean academy of pediatric dentistry
Abbr : J Korean Acad Pediatr Dent
2020, 47(1), pp.70-77
DOI : 10.5933/JKAPD.2020.47.1.70
Publisher : The Korean Academy Of Pediatric Dentistry
Research Area : Medicine and Pharmacy > Dentistry
Received : September 8, 2019
Accepted : November 4, 2019
Published : February 29, 2020

Choa Park ¹, Howon Park ², LEE JU HYUN ², Hyunwoo Seo ², Lee, Si Young ²

¹강릉원주대학교 치과대학 소아치과학교실 및 구강과학연구소
²강릉원주대학교

Accredited

ABSTRACT

The present study is aimed to assess the effect of antimicrobial photodynamic therapy (aPDT) on Streptococcus mutans biofilm through teeth whitening light emitting diode (LED). Planktonic and dynamic biofilm state cultures of S. mutans were used. Erythrosine 20 μM/L was used as the photosensitizer. Irradiation was performed by exposing cultures to clinic and homecare whitening LEDs for 15 minutes. The viability was measured through Colony Forming Unit counts and confocal laser scanning microscopy. aPDT using whitening LEDs and erythrosine significantly decreased the CFU count of S. mutans compared to that in the control group. Dynamic biofilm group showed more resistant features to aPDT compared with planktonic state. Clinic and homecare whitening LED device showed similar antimicrobial effect. The whitening LED, which could irradiate the entire oral arch, showed a significant photodynamic effect on cariogenic S. mutans biofilm. aPDT mediated by erythrosine and LEDs used for teeth whitening exhibited promising antimicrobial activity.

KEYWORDS

Photodynamic therapy, Whitening LED, Erythrosine, Streptococcus mutans

Citation status

* References for papers published after 2023 are currently being built.

[journal] Bowen WH / 2002 / Do we need to be concerned about dental caries in the coming millennium? / Crit Rev Oral Biol Med 13 : 126~131

[journal] Bowen WH / 2011 / Biology of Streptococcus mutans-derived glucosyltransferases : role in extracellular matrix formation of cariogenic biofilms / Caries Res 45 : 69~86

[journal] Marsh PD / 2010 / Controlling the oral biofilm with antimicrobials / J Dent 38 : S11~15

[journal] Van Straten D / 2017 / Oncologic photodynamic therapy : Basic principles, current clinical status and future directions / Cancers(Basel) 9 : E19~

[journal] Dolmans DE / 2003 / Photodynamic therapy for cancer / Nat Rev Cancer 3 : 380~387

[journal] Hamblin MR / 2016 / Antimicrobial photodynamic inactivation : a bright new technique to kill resistant microbes / Curr Opin Microbiol 33 : 67~73

[journal] Cieplik F / 2018 / Antimicrobial photodynamic therapy-what we know and what we don’t / Crit Rev Microbiol 44 : 571~589

[journal] Konopka K / 2007 / Photodynamic therapy in dentistry / J Dent Res 86 : 694~707

[journal] Tahmassebi JF / 2015 / A study of the control of oral plaque biofilms via antibacterial photodynamic therapy / Eur Arch Paediatr Dent 16 : 433~440

[journal] Nagata JY / 2012 / Antibacterial photodynamic therapy for dental caries : Evaluation of the photosensitizers used and light source properties / Photodiagnosis Photodyn Ther 9 : 122~131

[journal] 김재용 / 2018 / Antimicrobial Effect on Streptococcus mutans in Photodynamic Therapy using Different Light Source / 대한소아치과학회지 / 대한소아치과학회 45(1) : 82~89

[journal] 최서정 / 2015 / Optimum Treatment Parameters for Photodynamic Antimicrobial Chemotherapy on Streptococcus mutans Biofilms / 대한소아치과학회지 / 대한소아치과학회 42(2) : 151~157

[journal] Lee YH / 2012 / The photodynamic therapy on Streptococcus mutans biofilms using erythrosine and dental halogen curing unit / Int J Oral Sci 4 : 196~201

[journal] Soares JA / 2018 / Exploring different photodynamic therapy parameters to optimize elimination of Enterococcus faecalis in planktonic form / Photodiagnosis Photodyn Ther 22 : 127~131

[journal] Pourhajibagher M / 2018 / Exploring different photosensitizers to optimize elimination of planktonic and biofilm forms of Enterococcus faecalis from infected root canal during antimicrobial photodynamic therapy / Photodiagnosis Photodyn Ther 24 : 206~211

[other] / Material Safety Data sheet of Erythrosine B / Chemicals &Laboratory Equipment

[journal] Wood S / 2006 / Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms / J Antimicrob Chemother 57 : 680~684

[journal] Mondelli RF / 2016 / Evaluation of temperature increase during in-office bleaching / J Appl Oral Sci 24 : 136~141

[journal] Malallah R / 2019 / Improving the uniformity of holographic recording using multi-layer photopolymer:Part II. Experimental results / J Opt Soc Am A Opt Image Sci Vis 36 : 334~344

[journal] de Sousa DL / 2015 / Effect of twice-daily blue light treatment on matrix-rich biofilm development / PLoS One 10 : e0131941~

[journal] Sedlacek MJ / 2007 / Antibiotic resistance in an in vitro subgingival biofilm model / Oral Microbiol Immunol 22 : 333~339

[journal] Fontana CR / 2009 / The antibacterial effect of photodynamic therapy in dental plaquederived biofilms / J Periodont Res 44 : 751~759

[journal] Baab DA / 1983 / A comparison of antimicrobial activity of four disclosant dyes / J Dent Res 62 : 837~841

[journal] Kato H / 2012 / Xanthene dyes induce membrane permeabilization of bacteria and erythrocytes by photoinactivation / Photochem Photobiol 88 : 423~431

[journal] Kim S / 2013 / In vitro bactericidal effects of 625, 525, and 425 nm wavelength(red, green, and blue)light-emitting diode irradiation / Photomed Laser Surg 31 : 554~562

[journal] Chebath-Taub D / 2012 / Influence of blue light on Streptococcus mutans re-organization in biofilm / J Photochem Photobiol B 116 : 75~78

[journal] Felix Gomez GG / 2018 / Effect of violet-blue light on Streptococcus mutans-induced enamel demineralization / Dent J(Basel) 6 : E6~

KJCKorea
Journal Central

Journal of the Korean academy of pediatric dentistry 2023 KCI Impact Factor : 0.48

Application of Teeth Whitening LED for Prevention of Dental Caries : Antimicrobial Photodynamic Therapy Approach

ABSTRACT

KEYWORDS

Citation status

* References for papers published after 2023 are currently being built.

Journal of the Korean academy of pediatric dentistry 2023 KCI Impact Factor : 0.48

Application of Teeth Whitening LED for Prevention of Dental Caries : Antimicrobial Photodynamic Therapy Approach

ABSTRACT

KEYWORDS

Statistics

Tools

Issue List

Citation status

KCI Citation Counts (1)

REFERENCES (27) * References for papers published after 2023 are currently being built.

Search PDF

Citation

* References for papers published after 2023 are currently being built.