Streptococcus Mutans Biofilm Inhibition Effect of Indocyanine Green and Near Infrared Diode Laser (Indocyanine Green과 근적외선 다이오드 레이저의 Streptococcus mutans 세균막에 대한 억제 효과)

Yeowon Kim (김여원); Howon Park (박호원); Juhyun Lee (이주현); Hyunwoo Seo (서현우); Siyoung Lee (이시영)

doi:10.5933/JKAPD.2020.47.4.446

Streptococcus Mutans Biofilm Inhibition Effect of Indocyanine Green and Near Infrared Diode Laser

Journal of the Korean academy of pediatric dentistry
Abbr : J Korean Acad Pediatr Dent
2020, 47(4), pp.446~453
DOI : 10.5933/JKAPD.2020.47.4.446
Publisher : The Korean Academy Of Pediatric Dentistry
Research Area : Medicine and Pharmacy > Dentistry
Received : July 7, 2020
Accepted : July 28, 2020
Published : November 30, 2020

Yeowon Kim ¹, Howon Park ², Juhyun Lee ², Hyunwoo Seo ², Siyoung Lee ²

¹강릉원주대학교 치과대학 소아∙청소년치과학교실 및 구강과학연구소
²강릉원주대학교

Accredited

ABSTRACT

The purpose of this study was to evaluate the effect of Indocyanine Green (ICG) and near-infrared (NIR) diode laser on Streptococcus mutans biofilms depending on ICG concentrations. S. mutans biofilms were formed on a Hydroxyapatite disk, and 0.5, 1.0, 2.0, 3.0, 4.0, 5.0 mg/mL ICG solutions dissolved in sterile distilled water and a NIR diode laser having a power of 300 mW and a wavelength of 808 nm were applied to the biofilms. The temperature changes of the biofilm surface according to the concentrations of the ICG solution were measured using a 1-channel thermocouple thermometer. Compared to the control group, in the groups with only the 3.0, 4.0, 5.0 mg/mL ICG solution application, and in the groups with the 1.0, 2.0, 3.0, 4.0, 5.0 mg/mL ICG solution application and light irradiation, a statistically significant decrease in the bacterial counts were observed. The temperature increase according to the concentration of the ICG solutions was 9.53℃, 10.43℃, 11.40℃, 12.10℃, 12.67℃, and 13.63℃ in ICG solutions of 0.5, 1.0, 2.0, 3.0, 4.0, and 5.0 mg/mL respectively. This study presents the potential for clinical application of ICG and NIR diode lasers as a new method for preventing dental caries.

KEYWORDS

Indocyanine Green, Near Infrared Diode laser, Streptococcus mutans, Biofilm, Photodynamic therapy, Photothermal therapy

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