2008, Vol.18, No.4

Neuroprotective Effects of Scrophulariae Radix on Cerebral Ischemia in Mongolian Gerbils

LEE JUN HWAN , Mi-Yeon Song , Jongsoo Lee and 3 other persons | 2008, 18(4) | pp.1~11 | number of Cited : 2

Effect of Poncirus Trifoliata on Colonic Motility in Spinal Cord Injured Rats

최철원 , Min Cheol Joo , Moon Young Lee | 2008, 18(4) | pp.13~24 | number of Cited : 1

The Effects of Ohyaksungi-san(Wuyaoshunqi-san) and Electrical Acupuncture on Nerve Regeneration after Crush Injury in Rat Sciatic Nerve

양미성 , Misook Shin , 안혜림 | 2008, 18(4) | pp.25~37 | number of Cited : 9

Studies of the Effects of Acupuncture Stimulation at Huatuo Jiaji(EX B2) Points on Axonal Regeneration of Injured Sciatic Nerve in the Rats

김대필 , 박영회 , Keum Dong-ho | 2008, 18(4) | pp.39~61 | number of Cited : 5

Objectives : The present study was performed to investigate whether acupuncture stimulation in the rats affected regeneration properties of the injured sciatic nerve. A differential effect of acupuncture stimulation on the one point near the spinal nerve root controlling sciatic nerve activity and the other point in the peripheral area subordinated by injured nerve was compared. Materials and Methods : Rat sciatic nerves were injured by crush, and the effects on axonal regeneration on injured sciatic nerves were evaluated by acupuncture stimulation at two different regions. In proximal acupuncture stimulation group, acupuncture stimulation was performed on Huatuo Jiaji(EX B2) points located from L5 to S1 vertebral levels to stimulate the nearest spinal nerve root that innervates sciatic nerves. In distal acupuncture stimulation group, acupuncture stimulation was performed on Zusanli(ST 36) and Weizhong(BL 40) points to stimulate at peripheral area dominated by injured sciatic nerves. Acupuncture stimulation was given every other days for 1 or 2 weeks. Sciatic nerve tissues collected from acupuncture stimulation experimental groups, injury control group, and intact animal group were used for protein analysis by Western blotting or Hoechst nuclear staining. To determine axonal regeneration, DiI fluorescence dye was injected into the sciatic nerve 0.5 cm distal to the injury site in individual animal groups and DiI-labeled cells by retrograde tracing were measured in the DRG at lumbar 5 or in the spinal cord. DRG sensory neurons prepared from individual animal groups were used to measure the extent of neurite outgrowth and for immunofluorescence staining with anti-GAP-43 antibody. Results : Animal groups given proximal or distal acupuncture stimulation showed upregulation of GAP-43 and Cdc2 protein levels in the sciatic nerve at 7 days after injury. Cdk2 protein levels were strongly induced by nerve injury, but did not show changes by acupuncture stimulation. Phospho-Erk1/2 protein levels were elevated by acupuncture stimulation above those present in the injury control animals. These increases in regeneration-associated protein levels appeared to be related with increased cell proliferation in the injured sciatic nerves. Hoechst 33258 staining of sciatic nerve tissue to visualize nuclei of individual cells showed increased Schwann cell number in the distal portion of the injured nerve 7 and 14 days after injury and further increases by acupuncture stimulation particularly at the proximal position. Measurement of axonal regeneration by retrograde tracing showed significantly increased DiI-labeled cells in proximal acupuncture stimulation group compared to distal acupuncture stimulation group and injury control group. Finally, an evaluation of axonal regeneration by retrograde tracing showed increased number of DiI labeled cells in the DRG at lumbar 5 or in the ventral horn of the spinal cord at lower thoracic level at 7 days after nerve injury. Conclusions : The present data show that the proximal acupuncture stimulation at Huatuo Jiaji(EX B2) points governing injured sciatic nerves was more effective for axonal regeneration than the distal acupuncture stimulation. Further studies on functional recovery or associated molecular mechanisms should be critical for developing animal models and clinical applications.

The Effect of Ecklonia Cava on Expressing of Blood Lipids and UCP-1 of Brown Adipose Tissue(BAT) in Zucker Rats

김현우 , Hojun Kim , 박영회 and 2 other persons | 2008, 18(4) | pp.63~72 | number of Cited : 0

Effects of Acupuncture at HT8⋅LR2 on Serum Lipid and Liver Function Induced by High Fat Diet in Rats

최태진 , 황문현 , 임선주 and 4 other persons | 2008, 18(4) | pp.73~83 | number of Cited : 3

The Study on Oriental-medical Understanding about Clinical Application of Craniosacral Techniques(CST)

정택근 , InSeon lee | 2008, 18(4) | pp.85~101 | number of Cited : 9

Review on Efficacy and Safety of Manipulation Therapy including Chuna Manipulation

김기병 , 박태용 , 이정한 and 5 other persons | 2008, 18(4) | pp.103~120 | number of Cited : 4

A Literature Review on the Clinical Application of Microcurrent Electrical Neuromuscular Stimulation(MENS) : articles published for recent 10 years

류지미 , Kim Sung-Soo , 김경석 and 1 other persons | 2008, 18(4) | pp.121~133 | number of Cited : 4

A Comparative Study with Lumbar Disc Herniation under Conservative Treatment according to the Duration

윤유석 , Jongsoo Lee , 하인혁 and 2 other persons | 2008, 18(4) | pp.135~145 | number of Cited : 4

The Study on Relation of Obesity and Low Back Pain Based on Body Composition Using Segmental Bioelectrical Impedance Analysis and Isokinetic Trunk Muscle Strength

박지현 , 안순선 , 최용훈 and 3 other persons | 2008, 18(4) | pp.147~159 | number of Cited : 2

A Comparative Study on the Changes of Pelvic Alignment between AP View of the Pelvis in Standing and Supine Position

이경무 , 박동수 , Kim， Soon-joong and 1 other persons | 2008, 18(4) | pp.161~169 | number of Cited : 7

The Retrospective Study on Modified Fasting Therapy in Inpatients

정순관 , 김동은 , 유덕선 and 3 other persons | 2008, 18(4) | pp.171~187 | number of Cited : 11

The Relationship between Abdominal Fat and Eating Attitude in Obese Climacteric Korean Women

이아라 , 황미자 , Chung Won Suk and 2 other persons | 2008, 18(4) | pp.189~201 | number of Cited : 1

Meridian-Electromyograph Analysis on Features of Abdominal Muscles in Chronic Low Back Pain Patients

정재영 , LEE JUN HWAN , 남기봉 and 1 other persons | 2008, 18(4) | pp.203~215 | number of Cited : 9

A Survey on the Injuries of Mixed Martial Arts Athletes : for Oriental Sports Medicine

박지훈 , 소갑석 , 박정현 and 3 other persons | 2008, 18(4) | pp.217~230 | number of Cited : 4

Two Cases Report of Spasmodic Torticollis by Acupuncture Therapy with Muscle Energy Techniques

박현호 , 정지은 , 정원희 and 2 other persons | 2008, 18(4) | pp.231~242 | number of Cited : 12

KJCKorea
Journal Central

Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6

2008, Vol.18, No.4