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Effect of Nardostachytis Rhizoma on Induction of Differentiation in HL-60 Cells

  • Journal of Korean Medicine Rehabilitation
  • Abbr : JKMR
  • 2007, 17(1), pp.61-74
  • Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine

윤상학 1 Seung-ryong Yeom 1 SONG YUNG SUN 1 Young Dal Kwon 1 박종하 1 김경수 1 오원교 1

1원광대학교

Candidate

ABSTRACT

Effect of Nardostachytis Rhizoma on Induction of Differentiation in HL-60 Cells Sang-Hak Yun, O.M.D., Jong-Ha Park, O.M.D., Kyeong-Su Kim, O.M.D., Won-Kyo Oh, O.M.D., Seung-Ryong Yeom, O.M.D., Young-Dal Kwon, O.M.D., Yung-Sun Song, O.M.D. Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University Objectives : To investigate PKC/MAPKs signaling pathways on the human promyelocytic leukemia HL-60 cell differentiation induced by Nardostachytis Rhizoma (N. Rhizoma). Methods : Differentiation of HL-60 cells was determined through expression of CD11b and CD14 surface antigens using flow cytometry and nitroblue tetrazolium (NBT) assay. PKC activity was measured by using a PKC Kinase Activity Assay kit (Non-Radioactive). Activation of ERK and protein levels of PKC isoforms were determined by Western blot analysis. Results : Activation of extracellular signal-regulated kinase (ERK) increased time-dependently in differentiation of HL-60 cells induced by N. Rhizoma. Inhibitor of ERK (PD98059) significantly reduced NBT reduction activity induced by N. Rhizoma in HL-60 cells. In addition, N. Rhizoma increased protein level of PKC α, PKC βI and PKC βII isoforms, but the protein level of PKC γ was constant. PKC inhibitors (GF 109203X, Chelerythrine and H-7) inhibited HL-60 cell differentiation and the ERK activation induced by N. Rhizoma. Conclusions : N. Rhizoma induces granulocytic differentiation via a PKC/ERK signaling pathway in HL-60 cells.

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