Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6
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pISSN : 1229-1854
- https://journal.kci.go.kr/jkmr
KJCKorea
Journal Central
Effects of Bee Venom Treatment on the Injured Ligament
- Journal of Korean Medicine Rehabilitation
- Abbr : JKMR
- 2007, 17(1), pp.75-98
- Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
- Research Area : Medicine and Pharmacy > Korean Medicine
이승민 1, Keum Dong-ho 1
1동국대학교
Candidate
ABSTRACT
Effects of Bee Venom Treatment on the Injured Ligament
Seung-Min Lee, O.M.D., Dong-Ho Keum, O.M.D.
Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Guk University
목적 :
손상된 인대조직이 염증기(Inflammation phase)에서 재형성기(Remodeling phase)로 이행되는 과정에서 봉독요법 치료가 손상 인대조직의 회복에 미치는 영향을 생화학적, 조직학적 방법으로 세포와 분자적인 관점에서 연구하고자 하였다.
방법 :
우측 뒷발의 족관절 염좌를 유발한 쥐들을 4주와 8주 후, 매주 1회 6주간 환측 족관절 전외측 부위(GB40, 구허)에 봉독요법을 시행한 후 인대조직을 박리하여 Western blotting, Immunofluorescence staining과 RT-PCR을 통하여 단백을 분석하였다. Hoechst 핵 염색법으로 세포수를 측정하였고 Masson's trichrome 염색법으로 콜라겐 섬유를 시각화하였다.
결과 :
봉독실험군에서 phospho-Erk1/2 kinase가 같은 기간 동안 손상대조군에 비해 증가되었다. 증가된 Erk1/2의 활성도는 Cyclin B1 단백질의 증가와 양성적인 관련을 보였고, 전체 세포수도 대조군보다 많았다. Type III collagen mRNA와 단백질은 봉독실험군에서 증가되었고 Trichrome staining에서도 증가된 collagen fiber를 확인할 수 있었다. TNF-α와 IL-1α mRNA 수준은 봉독실험군에서 손상대조군에 비해 현저한 감소를 보였다.
결론 :
인대 손상에 봉독요법을 시행한 결과 세포 증식 및 증가된 세포 활성을 통해 인대조직의 회복을 촉진함을 예측할 수 있었다.
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