Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6
-
pISSN : 1229-1854
- https://journal.kci.go.kr/jkmr
KJCKorea
Journal Central
Effects of Bee Venom Treatment on the Injured Ligament
- Journal of Korean Medicine Rehabilitation
- Abbr : JKMR
- 2007, 17(1), pp.75-98
- Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
- Research Area : Medicine and Pharmacy > Korean Medicine
이승민 1, Keum Dong-ho 1
1동국대학교
Candidate
ABSTRACT
Effects of Bee Venom Treatment on the Injured Ligament
Seung-Min Lee, O.M.D., Dong-Ho Keum, O.M.D.
Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Guk University
목적 :
손상된 인대조직이 염증기(Inflammation phase)에서 재형성기(Remodeling phase)로 이행되는 과정에서 봉독요법 치료가 손상 인대조직의 회복에 미치는 영향을 생화학적, 조직학적 방법으로 세포와 분자적인 관점에서 연구하고자 하였다.
방법 :
우측 뒷발의 족관절 염좌를 유발한 쥐들을 4주와 8주 후, 매주 1회 6주간 환측 족관절 전외측 부위(GB40, 구허)에 봉독요법을 시행한 후 인대조직을 박리하여 Western blotting, Immunofluorescence staining과 RT-PCR을 통하여 단백을 분석하였다. Hoechst 핵 염색법으로 세포수를 측정하였고 Masson's trichrome 염색법으로 콜라겐 섬유를 시각화하였다.
결과 :
봉독실험군에서 phospho-Erk1/2 kinase가 같은 기간 동안 손상대조군에 비해 증가되었다. 증가된 Erk1/2의 활성도는 Cyclin B1 단백질의 증가와 양성적인 관련을 보였고, 전체 세포수도 대조군보다 많았다. Type III collagen mRNA와 단백질은 봉독실험군에서 증가되었고 Trichrome staining에서도 증가된 collagen fiber를 확인할 수 있었다. TNF-α와 IL-1α mRNA 수준은 봉독실험군에서 손상대조군에 비해 현저한 감소를 보였다.
결론 :
인대 손상에 봉독요법을 시행한 결과 세포 증식 및 증가된 세포 활성을 통해 인대조직의 회복을 촉진함을 예측할 수 있었다.
KEYWORDS
Statistics
-
423 Viewed
-
0 Downloaded
Tools
Issue List
Citation status
* References for papers published after 2023 are currently being built.
[journal]
/ 2001
/ The analgesic efficacy of bee venom acupuncture for knee osteoarthritis: a comparative study with needle acupuncture
29(2)
: 187~199
[journal]
/ 2005
/ An Overview of Bee Venom Acupuncture in the Treatment of Arthritis
2(1)
: 79~84
[journal]
/ 2000
/ Treatments for fatigue in multiple sclerosis: a rapid and systematic review
4(27)
: 1~61
[journal]
/ 2003
/ The review on the study of bee venom in the domestic papers
20
: 154~165
[journal]
/ 1996
/ The bee venom test: a new tonic-pain test
66(2-3)
: 271~277
[journal]
/ 2003
/ 5-hydroxytryptamine1A receptor is involved in the bee venom induced inflammatory pain
106(1-2)
: 135~142
[journal]
/ 2003
/ Analysis of the inflammatory response in the rat paw caused by the venom of Apis melifera bee
52(3)
: 132~139
[journal]
/ 1973;245
/ An anti-inflammatory peptide from bee venom
: 163~4
[journal]
/ 2004
/ Acupoint stimulation with diluted bee venom (apipuncture) alleviates thermal hyperalgesia in a rodent neuropathic pain model: involvement of spinal alpha 2-adrenoceptors
5(6)
: 297~303
[journal]
/ 2003
/ The roles of growth factors in tendon and ligament healing
33(5)
: 381~394
[journal]
/ 1999
/ Molecular biology and biomechanics of normal and healing ligaments--a review
7(1)
: 130~140
[journal]
/ 1996
/ Phospholipase A2 activity is required for regeneration of sensory axons in cultured adult sciatic nerves
43(2)
: 183~189
[journal]
/ 2000
/ Contralateral heat hyperalgesia induced by unilaterally intraplantar bee venom injection is produced by central changes: a behavioral study in the conscious rat
284(1-2)
: 45~48
[journal]
/ 2002
/ Acupuncture analgesia in a new rat model of ankle sprain pain
99(3)
: 423~431
[journal]
/ 2004
/ Anti-inflammatory effect of bee venom on type II collagen-induced arthritis
32(3)
: 361~367
[journal]
/ 2001
/ Bee venom injection into an acupuncture point reduces arthritis associated edema and nociceptive responses
90(3)
: 271~280
[journal]
/ 2005
/ Unilateral subcutaneous bee venom but not formalin injection causes contralateral hypersensitized wind-up and after-discharge of the spinal withdrawal reflex in anesthetized spinal rats
195(1)
: 148~160
[journal]
/ 2001
/ Scavenging effect of bee venom for acua- acupunture against nitric oxide
18
: 161~170
[journal]
/ 2003
/ Effects of bee venom on cell proliferation and cyclooxygease 2 expression in the dentate gyrus of mice with acetic acid induced hyperalgesia
20
: 112~122
[journal]
/ 2003
/ Effects of bee venom on the pain, edema, and acute inflammatory reactants of rheumatoid arthritis patients
20
: 77~84
[journal]
/ 1984
/ Lateral ligamentous injuries of the ankle Traumatic disorders of the ankle
: 113~23
[journal]
/ 1999
/ Primary care orthopedics
/ St. Louis, MO:Mosby
: 306~380
[journal]
/ 1999
/ Ankle sprain: clinical evaluation and current treatment concept
/ Philadelphia, PA:Churchill Livingston
: 323~340
[journal]
/ 2000
/ Signal transduction by the JNK group of MAP kinases
103(2)
: 239~252
[journal]
/ 2003
/ MAPK pathways in radiation responses
22(37)
: 5885~5896
[journal]
/ 1995
/ Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis
270(5240)
: 1326~1331
[journal]
/ 1999
/ Extracellular- signal-regulated kinase signalling in neurons
9(5)
: 544~553
[journal]
/ 1996
/ Intracellular signaling pathways activated by neurotrophic factors
19
: 463~489
[journal]
/ 2005
/ Recent advances in the protein kinase B signaling pathway
17(2)
: 150~157
[journal]
/ 2000
/ Akt/protein kinase B prevents injury-induced motoneuron death and accelerates axonal regeneration
20(8)
: 2875~2886
[journal]
/ 2005
/ Vimentin- dependent spatial translocation of an activated MAP kinase in injured nerve
45(5)
: 715~726
[journal]
/ 2003
/ Fas engagement induces neurite growth through ERK activation and p35upregulation
5(2)
: 118~125
[journal]
/ 2004
/ Cdk5 phosphorylation of FAK regulates centrosome-associated miocrotubules and neuronal migration
3(2)
: 108~110
[journal]
/ 2001
/ A decade of CDK5
2(10)
: 749~759
[journal]
/ 2003
/ Cyclin B1 and CDK1: nuclear localization and upstream regulators
5
: 335~347
[journal]
/ 1997
/ Mammalian G1 and G2 phase checkpoints
29
: 151~182
[journal]
/ 1994;8
/ The cyclin-dependent protein kinases and the control of cell division
: 1114~21
[journal]
/ 1996
/ Newly assembled cyclin B-cdc2 kinase is required to suppress DNA replication between meiosis I and meiosis II in starfish oocytes
15
: 3590~3598
[journal]
/ 1999
/ ERK pathway mediates the activation of Cdk2 in IGF-1-induced proliferation of human osteosarcoma MG-63 cells
14(4)
: 528~535
[journal]
/ 2004
/ Disialoganglioside (GD3) synthase gene expression suppresses vascular smooth muscle cell responses via the inhibition of ERK1/2 phosphorylation, cell cycle progression, and matrix metalloproteinase-9 expression
279(32)
: 33063~33070
[journal]
/ 2002
/ Phosphorylation of MEK1 by cdk5/p35 down-regulates the mitogen- activated protein kinase pathway
277(1)
: 528~534
[journal]
/ 1999
/ Microtubules and signal transduction
11(1)
: 81~94
[journal]
/ 2005
/ Antimitogenic effect of green tea -epigallocatechin gallate on 3T3-L1preadipocytes depends on the ERK and Cdk2 pathways
288(5)
: C1094~C1108
[journal]
/ 2004
/ The MAP kinase pathway is required for entry into mitosis and cell survival
23(3)
: 763~776
[journal]
/ 2005.01-02
/ Modulation of proliferation and differentiation of human bone marrow stromal cells by fibroblast growth factor 2: potential implications for tissue engineering of tendons and ligaments
11(1-2)
: 41~49
[journal]
/ 2003
/ Basic fibroblast growth factor induces the expression of matrixmetalloproteinase-3 in human periodontal ligament cells through the MEK2 mitogen- activated protein kinase pathway
38(2)
: 122~129
[journal]
/ 2002
/ Immunohistochemical evaluation of matrix molecules associated with wound healing following regenerative periodontal treatment in monkeys
6(3)
: 175~182
[journal]
/ 2003
/ Immunohistochemical evaluation of matrix molecules associated with wound healing following treatment with an enamel matrix protein derivative in humans
7(3)
: 167~174
[journal]
/ 1996
/ Biologic basis for interleukin-1 in disease
87(6)
: 2095~2147
[journal]
/ 2001
/ Chemokines in pathology and medicine
250(2)
: 91~104
[journal]
/ 2002
/ Points of control in inflammation
420
: 846~852
[journal]
/ 2005
/ The use of therapeutic medications for soft-tissue injuries in sports medicine
183(7)
: 384~388
[journal]
/ 2005
/ Intraligamentous injection of sclerosing solutions (prolotherapy) for spinal pain: a critical review of the literature
5(3)
: 310~328
[journal]
/ 2004
/ Prolotherapy injections for chronic low back pain: a systematic review
29(19)
: 2126~2133
[journal]
/ 1999
/ Inducible cyclooxygenase may have anti-inflammatory properties
5(6)
: 698~701
[journal]
/ 2003
/ The two faces of IKK and NF-kappa B inhibition: prevention of systemic inflammation but increased local injury following intestinal ischemia- reperfusion
9(5)
: 575~581
[journal]
/ 2001
/ Possible new role for NF- kappaB in the resolution of inflammation
7(12)
: 1291~1297
[journal]
/ 2001
/ Prostaglandins and leukotrienes: advances in eicosanoid biology
294(5548)
: 1871~1875
[journal]
/ 2000
/ COX-2- Specific inhibitors--the emergence of a new class of analgesic and anti-inflammatory drugs
19(5)
: 331~343
[journal]
/ 1978;16
/ Stimulation of prostaglandin production in bone by phorbol diesters and melittin
: 221~32