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Inhibitory Effects of Gamidaeganghwal-tang(Jiaweidaqianghuo-tang) on Rheumatoid Arthritis Induced by Type Ⅱ Collagen

  • Journal of Korean Medicine Rehabilitation
  • Abbr : JKMR
  • 2009, 19(2), pp.89-102
  • Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine

김민기 1 Minseok Oh 1

1대전대학교

Accredited

ABSTRACT

Objectives : This study was carried out to understand the immunity responses and anti-oxidation effect of the Gamidaeganghwal-tang(GDT) on rheumatoid arthritis by using the THP-1 cells and the serum of CIA mice. Methods : For this purpose, GDT was orally administerd to mice with rheumatoid arthritis induced by collagen Ⅱ. To investigate the immunity responses, value of cytokine and gene expression in the THP-1 cell, levels of cytokines in the serum of CIA(collagen type II induced arthritis) mice, number of immunocyte in PBMC of CIA mice were measured. Then, anti-oxidant activity, scavenging activity on DHHP(2,2-diphenyl-1-picrylhydrazyl) free radical and SOD(Superoxide dismutae)-like activity of GDT was observed. Results : 1. The levels of IL-1β, IL-6, IL-8, MCP-1 at 100, 50 ㎍/㎖ of GDT were significantly reduced in the THP-1 cell. 2. The levels of TNF-α, COX-2 mRNA expression at 100, 50 ㎍/㎖ of GDT and IL-1β, IL-6 at 100 ㎍/㎖ of GDT were significantly reduced in the THP-1 cell line. 3. The levels of IL-6, TNF-α and IL-1β were significantly reduced in the serum of CIA mice. 4. The absolute number of CD3+, CD4+ and CD8+ cells were significantly induced, CD3+/CD69+, CD3+/CD49+, CD19+, B220+/CD23+ cells were significantly reduced in PBMC. 5. Scavenging activity on DPPH free radical and SOD-like activity were significantly induced in a concentration dependent manner. Conclusions : Taking all these observations, GDT considered to be effective in treating rheumatoid arthritis. Therefore we have to survey continuously in looking for the effective substance and mechanism in the future.

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