Inhibitory Effects of Gamidaeganghwal-tang(Jiaweidaqianghuo-tang) on Rheumatoid Arthritis Induced by Type Ⅱ Collagen (加味大羌活湯이 Collagen Ⅱ로 유발된 관절염에 미치는 영향 )

김민기; Minseok Oh (오민석)

Inhibitory Effects of Gamidaeganghwal-tang(Jiaweidaqianghuo-tang) on Rheumatoid Arthritis Induced by Type Ⅱ Collagen

Journal of Korean Medicine Rehabilitation
Abbr : JKMR
2009, 19(2), pp.89-102
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Medicine and Pharmacy > Korean Medicine

김민기 ¹, Minseok Oh ¹

¹대전대학교

Accredited

ABSTRACT

Objectives : This study was carried out to understand the immunity responses and anti-oxidation effect of the Gamidaeganghwal-tang(GDT) on rheumatoid arthritis by using the THP-1 cells and the serum of CIA mice. Methods : For this purpose, GDT was orally administerd to mice with rheumatoid arthritis induced by collagen Ⅱ. To investigate the immunity responses, value of cytokine and gene expression in the THP-1 cell, levels of cytokines in the serum of CIA(collagen type II induced arthritis) mice, number of immunocyte in PBMC of CIA mice were measured. Then, anti-oxidant activity, scavenging activity on DHHP(2,2-diphenyl-1-picrylhydrazyl) free radical and SOD(Superoxide dismutae)-like activity of GDT was observed. Results : 1. The levels of IL-1β, IL-6, IL-8, MCP-1 at 100, 50 ㎍/㎖ of GDT were significantly reduced in the THP-1 cell. 2. The levels of TNF-α, COX-2 mRNA expression at 100, 50 ㎍/㎖ of GDT and IL-1β, IL-6 at 100 ㎍/㎖ of GDT were significantly reduced in the THP-1 cell line. 3. The levels of IL-6, TNF-α and IL-1β were significantly reduced in the serum of CIA mice. 4. The absolute number of CD3+, CD4+ and CD8+ cells were significantly induced, CD3+/CD69+, CD3+/CD49+, CD19+, B220+/CD23+ cells were significantly reduced in PBMC. 5. Scavenging activity on DPPH free radical and SOD-like activity were significantly induced in a concentration dependent manner. Conclusions : Taking all these observations, GDT considered to be effective in treating rheumatoid arthritis. Therefore we have to survey continuously in looking for the effective substance and mechanism in the future.

KEYWORDS

Gamidaeganghwal-tang(Jiaweidaqianghuo-tang, GDT), Inflammatory cytokine, Antioxidant activity, Rheumatoid arthritis

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