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@article{ART001339422},
author={이상현 and Yun Yong-Gab},
title={Effect of Korea Red Ginseng Extract on PC12 Cell Death Induced by Serum Deprivation},
journal={Journal of Korean Medicine Rehabilitation},
issn={1229-1854},
year={2009},
volume={19},
number={2},
pages={103-112}

TY - JOUR
AU - 이상현
AU - Yun Yong-Gab
TI - Effect of Korea Red Ginseng Extract on PC12 Cell Death Induced by Serum Deprivation
JO - Journal of Korean Medicine Rehabilitation
PY - 2009
VL - 19
IS - 2
PB - The Korean Academy Of Oriental Rehabilitation Medicine
SP - 103
EP - 112
SN - 1229-1854
AB - Objectives : This study was to evaluate the pharmacological effect of Korea Red Ginseng aqueous extract (KRGE) on serum-deprived apoptosis of neuronal-like pheochromocytoma PC12 cells and to investigate its underlying action mechanism. Methods : KRGE was prepared by extracting Korea Red Ginseng with hot water and concentrating using a vacuum evaporator. Cell viability was determined after incubation of cells with KRGE or chemical inhibitor in serum-deprived medium for 60 h by counting intact nuclei following lysing of the cell membrane. Caspase activities were measured using chromogenic substrates and signal-associated protein phosphorylation and cytochrome c release were determined by Western blot analyses using their specific antibodies. Results : Serum deprivation induced PC12 cell death, which was accompanied by typical morphological features of apoptotic cell, such as nuclear fragmentation, caspase-3 activation, and cytochrome c release. This apoptotic cell death was significantly inhibited by KRGE and caspase-3 inhibitor, but not by the addition of NMA, ODQ, and PD98059. KRGE promoted phosphorylation of Akt and Bad, and this phosphorylation was inhibited by the PI3K inhibitor LY92004. In addition, this inhibitor also reversed KRGE-mediated protection of PC 12 cells from serum deprivation. These results suggested that KRGE protects PC12 cells from serum deprivation-induced apoptosis through the activation of PI3K/Akt-dependent Bad phosphorylation and cytochrome c release, resulting in caspase-3 activation. Conclusions : KRGE should be considered as a potential therapeutic drug for brain diseases including stroke induced by apoptosis of neuronal cells.
KW - Korea Red Ginseng;Apoptosis;Caspase;Neuronal Cells;Stroke
DO -
UR -
ER -

이상현 and Yun Yong-Gab. (2009). Effect of Korea Red Ginseng Extract on PC12 Cell Death Induced by Serum Deprivation. Journal of Korean Medicine Rehabilitation, 19(2), 103-112.

이상현 and Yun Yong-Gab. 2009, "Effect of Korea Red Ginseng Extract on PC12 Cell Death Induced by Serum Deprivation", Journal of Korean Medicine Rehabilitation, vol.19, no.2 pp.103-112.

이상현, Yun Yong-Gab "Effect of Korea Red Ginseng Extract on PC12 Cell Death Induced by Serum Deprivation" Journal of Korean Medicine Rehabilitation 19.2 pp.103-112 (2009) : 103.

이상현, Yun Yong-Gab. Effect of Korea Red Ginseng Extract on PC12 Cell Death Induced by Serum Deprivation. 2009; 19(2), 103-112.

이상현 and Yun Yong-Gab. "Effect of Korea Red Ginseng Extract on PC12 Cell Death Induced by Serum Deprivation" Journal of Korean Medicine Rehabilitation 19, no.2 (2009) : 103-112.

이상현; Yun Yong-Gab. Effect of Korea Red Ginseng Extract on PC12 Cell Death Induced by Serum Deprivation. Journal of Korean Medicine Rehabilitation, 19(2), 103-112.

이상현; Yun Yong-Gab. Effect of Korea Red Ginseng Extract on PC12 Cell Death Induced by Serum Deprivation. Journal of Korean Medicine Rehabilitation. 2009; 19(2) 103-112.

이상현, Yun Yong-Gab. Effect of Korea Red Ginseng Extract on PC12 Cell Death Induced by Serum Deprivation. 2009; 19(2), 103-112.

이상현 and Yun Yong-Gab. "Effect of Korea Red Ginseng Extract on PC12 Cell Death Induced by Serum Deprivation" Journal of Korean Medicine Rehabilitation 19, no.2 (2009) : 103-112.