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Study on Effect of Saengbal-eum-II(Shēngfà-yĭn-Ⅱ) on Hair Regrowth Promotion in C57BL/6 Mice

  • Journal of Korean Medicine Rehabilitation
  • Abbr : JKMR
  • 2009, 19(4), pp.95-114
  • Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine

한애리 1,  SOHN NAK-WON 1,  Chung, Seok Hee 1,  Kim Sung-Soo 1,  Mi-Yeon Song 1

1경희대학교

Accredited

ABSTRACT

Objectives : Saengbal-eum-II(Shēngfà-yĭn-II) is a hair care product which is composed of ten plant extracts used in oriental medicine. This study was carried out to investigate the effects of Saengbal-eum-II(Shēngfà-yĭn-II) on hair regrowth and cytokine changes in a shaving model of C57BL/6 mice. Materials and Methods : Five-week-old mice were acclimated for 1 week at a temperature between 21-23℃, 40-60% relative humidity, and 12h of a light/dark cycle before beginning of the experiment. There were three experimental groups including 50% ethanol (EtOH, control), a positive control of 3% Minoxidil, and 30% Saengbal-eum-II(Shēngfà-yĭn-II) in 50% ethanol in 18 female mice. The test compounds were topically treated once a day over 12 days. The hair regrowth was photographically and histologically determined during the experimental period of 12 days. Revelation of EGF, TGF-β1 and IL-6 in hair follicle were also determined using immunohistochemistry. In addition to that, IL-6, TNF-α, and IL-1β in skin tissue were determined using enzyme-linked immunosorbent assay(ELISA). Results : Hair regrowth in 3% Minoxidil and Saengbal-eum-II(Shēngfà-yĭn-II) groups was promoted earlier and faster than the control group. Concentrations of hairs and thick-hair ratio in 3% Minoxidil and Saengbal-eum-II(Shēngfà-yĭn-II) groups were promoted than the control group. EGF was moderately positive in hair follicle of 3% Minoxidil and Saengbal-eum-II(Shēngfà-yĭn-II) groups, but negative in the control group. TGF-β1 was not significantly difference between the groups. IL-6 in hair follicle of Saengbal-eum-II(Shēngfà-yĭn-II) group was negative, but weakly positive in 3% Minoxidil and control group. IL-6 and IL-1β in skin tissue were significantly decreased in Saengbal-eum-II(Shēngfà-yĭn-II) group, but there was not significantly decreased in 3% Minoxidil and control group. TNF-α in skin tissue was significantly decreased in 3% Minoxidil and Saengbal-eum-II(Shēngfà-yĭn-II) groups. Conclusions : These results suggest that Saengbal-eum-II(Shēngfà-yĭn-II) has hair growth promoting activity and it can be used for treatment of alopecia. And these effects relate to EGF revelation of hair follicle and a decrease IL-6, TNF-α, and IL-1β in skin tissue.

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