Effects of Hwangryunhaedok-tang(Huanglianjiedu-tang ) on Locomotor Dysfunction of Contusive Spinal Cord Injury-induced Rats (黃連解毒湯이 척수 압박손상 흰쥐의 운동기능 장애에 미치는 영향 )

성주원; Ki-Yuk Kim (김기역); 반효정; Jung-Won Shin (신정원); 강희; Seong-Joon Kim (김성준); SOHN NAK-WON (손낙원)

Effects of Hwangryunhaedok-tang(Huanglianjiedu-tang ) on Locomotor Dysfunction of Contusive Spinal Cord Injury-induced Rats

Journal of Korean Medicine Rehabilitation
Abbr : JKMR
2010, 20(4), pp.1-15
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Medicine and Pharmacy > Korean Medicine

성주원 ¹, Ki-Yuk Kim ¹, 반효정 ¹, Jung-Won Shin ¹, 강희 ¹, Seong-Joon Kim ², SOHN NAK-WON ¹

¹경희대학교
²경희대학교 동서의학대학원 한의과학전공

Accredited

ABSTRACT

Objectives :This study was performed to evaluate the effects of Hwangryunhaedok-tang(Huanglianjiedu-tang; HHT) water extract on locomotor dysfunction induced by spinal cord injury(SCI) in rats. Methods :SCI was induced by mechanical contusion following laminectomy of 10th thoracic vertebra in Sprague-Dawley rats. HHT was orally given once a day for 14 days after SCI. Neurological behavior was examined with the Basso-Beattie-Bresnahan locomotor rating scale. Tissue damage and nerve fiber degeneration were examined with cresyl violet and luxol fast blue staining. Using immunohistochemistry, cellular damage to neurons and nerve fibers were examined against Bax and MAP-2. As inflammatory response markers, iNOS and COX-2 expressions were also examined. Results :1. HHT ameliorated the locomotor dysfunction of the SCI-induced rats. 2. HHT attenuated the reduction of motor neurons in the ventral horn of the SCI-induced rat spinal cord. 3. HHT significantly reduced the number of Bax positive cells in the peri-lesion of the SCI-induced rat spinal cord. 4. HHT attenuated the reduction of MAP-2 positive cells in the peri-lesion of the SCI-induced rat spinal cord. 3. HHT significantly reduced the number of iNOS and COX-2 positive cells in the peri-lesion of the SCI-induced rat spinal cord. Conclusions :These results suggest that HHT improves the locomotor dysfunction of SCI by protecting motor neurons from cell death through anti-inflammatory effect.

KEYWORDS

Hwangryunhaedok-tang(Huanglianjiedu-tang), Locomotor dysfunction, Spinal cord injury, Bax, MAP-2, iNOS, COX-2

Citation status

* References for papers published after 2023 are currently being built.

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