An Immunohistochemical Study on Effect of Gastrodiae Rhizoma against Neuronal Apoptosis Following Intracerebral Hemorrhage in Rats (天麻가 뇌실질출혈 흰쥐의 신경세포 자연사에 미치는 영향에 대한 면역조직화학적 연구)

송상훈; 이준석; 신정원; Seong-Joon Kim (김성준); SOHN NAK-WON (손낙원)

An Immunohistochemical Study on Effect of Gastrodiae Rhizoma against Neuronal Apoptosis Following Intracerebral Hemorrhage in Rats

Journal of Korean Medicine Rehabilitation
Abbr : JKMR
2011, 21(4), pp.1-12
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Medicine and Pharmacy > Korean Medicine

송상훈 ¹, 이준석 ¹, 신정원 ¹, Seong-Joon Kim ¹, SOHN NAK-WON ¹

¹경희대학교

Accredited

ABSTRACT

Objectives :This study was carried out in order to examine the effects of Gastrodiae rhizoma(GR) ethanol extract on neuronal apoptosis in intracerebral hemorrhage(ICH)-induced rats. Methods :ICH was induced by the stereotaxic intrastriatal injection of bacterial collagenase type VII in Sprague-Dawley rats. GR was orally given once a day for 3 days after ICH. Histological changes of the peri-hematoma regions were observed by cresyl violet staining. Bcl-2-associated X protein(Bax), B-cell blastoma 2(Bcl-2) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling(TUNEL) expressions in the affected regions were performed by immunohistochemistry. Results :1. GR reduced apoptotic bodies and swelling neurons in the peri-hematoma regions of ICH-induced rats. 2. GR significantly reduced TUNEL positive cells in the peri-hematoma regions of ICH-induced rats. 3. GR significantly reduced Bax positive cells in the peri-hematoma regions of ICH-induced rats. 4. GR did not influence Bcl-2 expression in the peri-hematoma regions of ICH-induced rats. Conclusions :These results suggest that GR has neuroprotective effects against ICH-induced apoptosis.

KEYWORDS

Intracerebral hemorrhage, Gastrodiae rhizoma, Neuronal apoptosis, TUNEL, Bax, Bcl-2

Citation status

* References for papers published after 2023 are currently being built.

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Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6