Effects of Scutellariae Radix on Neuronal Apoptosis of Spinal Cord Contusion Injury in Rats (黃芩이 척수압박손상 흰쥐의 척수신경세포 자연사에 미치는 영향)

반효정; 김범회; 신정원; 박성하; Seong-Joon Kim (김성준); SOHN NAK-WON (손낙원); Jong-Jin Jo (조종진)

Effects of Scutellariae Radix on Neuronal Apoptosis of Spinal Cord Contusion Injury in Rats

Journal of Korean Medicine Rehabilitation
Abbr : JKMR
2011, 21(4), pp.13-22
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Medicine and Pharmacy > Korean Medicine

반효정 ¹, 김범회 ², 신정원 ¹, 박성하 ³, Seong-Joon Kim ¹, SOHN NAK-WON ¹, Jong-Jin Jo ⁴

¹경희대학교
²동의대학교
³부산대학교
⁴자생한방병원

Accredited

ABSTRACT

Objectives :This study was performed to evaluate the effects of Scutellariae Radix(SR) water-extract on the tissue and neuronal apoptosis of the spinal cord injury(SCI). Methods :SCI was induced by mechanical contusion following laminectomy of 10th thoracic vertebra in Sprague-Dawley rats. SR was orally given once a day for 7 days after SCI. Neuronal apoptosis was examined with terminal deoxynucleotidyl transferase-mediated dUTPnick-end labeling(TUNEL) assay. Bax (Bcl-2-asociated X protein), Bcl-2(B-cell blastoma 2), c-Fos(FBJ osteosarcoma oncogene) expressions were examined using immuno-histochemistry. Individual TUNEL and immuno-labeled cells expressing Bax, Bcl-2 and c-Fos were counted on the same level in peri-damaged region and in ventral horn. Results :1. SR significantly reduced number of TUNEL labeled apoptotic cells induced by the spinal cord contusion injury. 2. SR significantly reduced Bax positive cells expression on the motor neuron in the ventral horn induced by the spinal cord contusion injury. 3. SR strengthened Bcl-2 expression on the motor neuron in the ventral horn induced by the spinal cord contusion injury. 4. SR reduced c-Fos expression on the motor neuron in the ventral horn induced by the spinal cord contusion injury. Conclusions :These results suggest that SR plays an inhibitory role against neuronal apoptosis and has significant effects for locomotor dysfunction induced by SCI.

KEYWORDS

Scutellariae Radix, Spinal cord injury, Apoptosis, TUNEL, Bax, Bcl-2

Citation status

* References for papers published after 2023 are currently being built.

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