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Protective Effect of Sorbus Commixta Cortex Extract on CoCl2-induced Apoptosis in Neuroblastoma Cell Line SK-N-MC

  • Journal of Korean Medicine Rehabilitation
  • Abbr : JKMR
  • 2012, 22(2), pp.17-29
  • Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine

Min-Soo Sung 1 Yun-Kyung Song 1 Lim Hyung Ho 2

1가천대학교 한의과대학 한방재활의학과교실
2가천대학교

Accredited

ABSTRACT

Objectives :Sorbus commixta cortex(SCC) has been traditionally used in Korea, China and Japan for the treatment of cough, asthma, and other bronchial disorders. Cobalt chloride(CoCl2) is used to induce hypoxic condition in vitro study. The purpose of this study was to evaluate the effects of SCC extract on CoCl2-induced apoptosis in human SK-N-MC neuroblastoma cells. Methods :In order to investigate whether SCC possesses a protective effect on CoCl2-induced cytotoxicity in neuronal cells, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, DAPI(4,6-diamidino-2-phenylindole) staining, TUNEL(terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) assay, DNA fragmentation assay, flow cytometry, DNA fragmentation assay, and Western blotting were performed on SK-N-MC neuroblastoma cells. Results :Cells treated with CoCl2 exhibited several apoptotic features, while cells pre-treated with SCC prior to CoCl2 exposure showed a decrease in the occurrence of apoptotic features. CoCl2 increased HIF-1α expression, however, SCC treatment decreased CoCl2-induced HIF-1α expression. The extract of SCC treatment suppressed Bax and caspase-3 expressions, and increased Bcl-2 expression in human neuroblastoma SK-N-MC cells. Conclusions :These results suggest that SCC may exert a protective effect on CoCl2-induced apoptosis in SK-N-MC neuroblastoma cells.

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