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Anti-OsteoArthritic effects of Mahwangbuja-tang(Mahuangfuzi-tang ) on Papain-Induced OsteoArthritic mice model

  • Journal of Korean Medicine Rehabilitation
  • Abbr : JKMR
  • 2012, 22(4), pp.59-77
  • Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine

장효길 1,  Dongseok Heo 1

1대전대학교

Accredited

ABSTRACT

Objectives :This study was to investigate the anti-osteoarthritic effects of Mahwangbuja-tang(Mahuangfuzi-tang, MBT) on the papain-induced osteoarthritis. Methods :Arthritis was induced by injection of papain into knee joints of rats. Arthritic rats were divided into four groups(Normal, control, JoinsⓇ, MBT). Control group was taken distilled water for 4 weeks and the MBT group, extracts of MBT by orally for 4 weeks. At the end of the experiment(5 weeks after papain injection), micro CT arthrography and histopathological examinations on the articular structures of knee joint were performed. The effect on inflammatory cytokine, immunological cell in synovial fluid was measured. Gene expressions of inflammatory cytokine in synovial fluid were measured by PCR. Volume of cartilage was measured by micro CT arthrography. Injury of synovial tissue was measured by H&E, safranin-O imunofluorescence. Results :1. IL-6, MCP-1, TNF-α were decreased significantly and IL-1β was decreased in MBT group. 2. Thromboxane B2, Leukotriene B4, prostaglandin E2 were decreased significantly in MBT group. 3. Neutrophil was decreased significantly and white blood cell, monocyte was decreased in MBT group. 4. IL-1β mRNA, iNOS-Ⅱ mRNA were decreased significantly and IL-6 mRNA, TNF-α mRNA, COX-2 mRNA were decreased in MBT group . 5. Through micro CT arthrography, volume of cartilage was increased significantly in MBT group. 6. Histopathologically, injury on synovial membrane of MBT group was decreased and proteoglycan content of MBT group was increased. Conclusions :According to this study, Mahwangbuja-tang has inhibiting effect on the progression of arthritis in papain-induced osteoarthritis model. And it is related to inhibiting the activity of inflammatory cytokine and its gene expression in osteoarthritic chondrocytes and injury of volume in cartilage.

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