Effects of Trachelospermum caulis Extract on Sodium Nitroprusside (SNP)-induced Inflammatory Responses in Rabbit HIG-82 Synovial Membrane Cells (낙석등 추출물이 토끼 HIG-82 활액막 세포주에서 Sodium Nitroprusside (SNP)로 유도된 염증반응에 미치는 영향)

박정식; Lim Hyung Ho (임형호)

Effects of Trachelospermum caulis Extract on Sodium Nitroprusside (SNP)-induced Inflammatory Responses in Rabbit HIG-82 Synovial Membrane Cells

Journal of Korean Medicine Rehabilitation
Abbr : JKMR
2014, 24(2), pp.31-40
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Medicine and Pharmacy > Korean Medicine

박정식 ¹, Lim Hyung Ho ¹

¹가천대학교 한의과대학 한방재활의학과교실

Accredited

ABSTRACT

Objectives Trachelospermi caulis, known as Nak-Suk-Deung in Korea, is the dried leafy stem of Trachelospermum asiaticum var. intermedium Nakai, and climbing stems and branches of Trachelospermum sdisyivum var, intermedium nakai or Apocyanaceae . Trachelospermi caulis has antipyretic and analgesic activity. It has traditionally been used as a folk remedy in Korea for the treatment of various infla mMatory diseases, including rheumatoid arthritis. The purpose of this study was to evaluate the Effects of Trachelospermum caulis extract on SNP-induced infla mMatory responses in rabbit HIG-82 synovial membrane cells. Methods Anti-infla mMatory effects of the extract of Trachelospermum caulis were investigated using rabbit HIG-82 synovial membrane cells. For this study, 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, western blot analysis, PGE2 i mMunoassay, and NO detection were conducted. Results The aqueous extract of Trachelospermum caulis exerted cytotoxicity and suppressed PGE2 synthesis and NO production in rabbit HIG-82 synovial membrane cells. The aqueous extract of Trachelospermum caulis also inhibited the SNP-induced expressions of COX-2, iNOS, and TNF-α in rabbit HIG-82 synovial membrane cells. Conclusions These results showed that the extract of Trachelospermum caulis exerts the anti-infla mMatory effect by suppressing COX-2, iNOS, and TNF-α expressions in the synovial membrane cells.

KEYWORDS

Trachelospermum caulis, infla mMation, COX-2, iNOS, TNF-α, PGE2, NO

Citation status

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[book] William F / 1999 / 생리학 / 한우리 : 681~

[journal] Kaplanski G / 2003 / IL-6 : a regulator of the transition from neutrophil to monocyte recruitment during infla mMation / Trends I mMunol 24(1) : 25~29

[journal] Kang-Rotondo CH / 1996 / Upregulation of nitric oxide synthase in cultured human keratinocytes after ultraviolet B and bradykinin / Photodermatol Photoi mMunol Photomed 12(2) : 57~65

[journal] Seo SJ / 2002 / Time course of expression of mRNA of inducible nitric oxide synthase and generation of nitric oxide by ultraviolet B in keratinocyte cell lines / Br J Dermatol 147(4) : 655~662

[journal] Shew RL / 1993 / NADPH-diaphorase-positive nerves and the role of nitric oxide in CGRP relaxation of uterine contraction / Peptides 14(3) : 637~641

[journal] Kawamata H / 2000 / Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS activated RAW 264.7 cells, a murine macrophage cell line / Am J Chin Med 28(2) : 217~226

[book] Kasper, Braunwald / 2006 / HARRISON'S 내과학 / 도서출판 MIP : 2148~2151

[journal] Matthew H Ornstein / 1996 / The antiinfla mMatory and antiviral effects of hydroxychloroquine in two patients with acquired i mMunodeficiency syndrome and active infla mMatory arthritis / Arthritis Rheum 39(1) : 157~161

[book] 전국한의과대학 본초학편집위원회 / 2005 / 본초학 / 영림사 : 319~320

[journal] 이태호 / 2009 / Effect of Trachelospermi Caulis Herbal-acupuncture on the Collagen-induced Arthritis in Rats / 대한침구의학회지 / 대한침구의학회 26(6) : 51~65

[journal] Tan XQ / 2010 / Chemical constituents of Trachelospermum jasminoides / Nat Prod Res 24(13) : 1248~1252

[journal] 신민교 / 1988 / 絡石藤과 地錦의 效能에 關한 書誌學的比較考察 / 대한본초학회지 13(1) : 37~43

[journal] 임형호 / 1991 / 絡石藤의 效能에 關한 實驗的硏究 / 경희의학 7(3) : 334~341

[journal] Lee MH / 2007 / In-vitro and in-vivo anti-infla mMatory action of the ethanol extract of Trachelospermi caulis / J Pharm Pharmacol 59(1) : 123~130

[journal] 정시화 / 2012 / Effect of Vigna angularis Ethanol Extract on Papain-induced Arthritis in Mice / 동의생리병리학회지 / 대한동의병리학회 26(5) : 665~671

[journal] Sarzi-Puttini P / 2005 / Osteoarthritis;an overview of the disease and its treatment strategies / Semin Arthritis Rheum 35(1) : 1~10

[other] 보건복지부 건강정책과 / 2012 / 2011 국민건강통계 / 보건복지부 : 512~

[journal] Cashman JN / 1996 / The mechanisms of action of NSAIDs in analgesia / Drugs 52(5) : 13~23

[journal] Duerksen-Hughes PJ / 1992 / Both tumor necrosis factor and nitric oxide participate in lysis of simian virus40-transformed cells by activated macrophages / J I mMunol 149(6) : 2114~2122

[journal] Crofford LJ / 2000 / Basic biology and clinical application of specific cyclooxygenase-2 inhibitors / Arthritis Rheum 43(1) : 4~13

[journal] Dawson TM / 1994 / Nitric oxide : cellular regulation and neuronal injury / Prog Brain Res 103 : 365~369

[journal] Nathan C / 1994 / Nitric oxide synthases : roles, tolls, and controls / Cell 78(6) : 915~918

[journal] 이경태 / 2007 / Genistein-4'-O-α-L-Rhamnopyranosyl-(1-2)-β-D-Glucopyranoside throughthe NF-κB Inactivation in RAW 264.7 Cells / 생약학회지 / 한국생약학회 38(4) : 339~348

[journal] McCartney-Francis N / 1993 / Suppression of arthritis by an inhibitor of nitric oxide synthase / J Exp Med 178(2) : 749~754

[journal] Badger AM / 1999 / Inhibition of interleukin-1 induced proteoglycan degradation and nitric oxide production in bovine articular cartilage/chondrocyte cultures by the natural product, hymenialdisine / J Pharmacol Exp Ther 290(2) : 587~593

[journal] Westacott CI / 1996 / Cytokines in osteoarthritis : mediators or makers of joint destruction / Semin Arthriris Rheum 25(4) : 254~327

[journal] Anggård E / 1994 / Nitric oxide : mediator, murderer, and medicine / Lancet 343(8907) : 1199~1206

[journal] Szabó C / 1995 / Alterations in nitric oxide production in various forms of circulatory shock / New Horiz 3(1) : 2~32

[journal] MacMicking J / 1997 / Nitric oxide and macrophage function / Annu Rev I mMunol 15 : 323~350

[journal] Ohshima H / 1994 / Chronic infections and infla mMatory processes as cancer risk factors : possible role of nitric oxide in carcinogenesis / Mutat Res 305(2) : 253~264

[journal] Kim KW / 2007 / Polygonum cuspidatum, compared with baicalin and berberine, inhibits inducible nitric oxide synthase and cyclooxygenase-2 gene expressions in RAW 264.7macrophages / Vascul Pharmacol 47(2,3) : 99~107

[journal] Förstermann, U / 1995 / Nitric oxide synthase : expression and expressional control of the three isoforms / Naunyn Schmiedebergs Arch Pharmacol 352(4) : 351~364

[journal] Barrera P / 1993 / Circulating soluble tumor necrosis factor receptors, interleukin-2 receptors, tumor necrosis factor alpha, and interleukin-6 levels in rheumatoid arthritis. Longitudinal evaluation during methotrexate and azathioprine therapy / Arthritis Rheum 36(8) : 1070~1079

[journal] Saxne T / 1988 / Detection of tumor necrosis factor a but not tumor necrosis factor β in rheumatoid arthritis synovial fluid and serum / Arthritis Rheum 31(8) : 1041~1045

[journal] Olsen NJ / 2004 / New drugs for rheumatoid arthritis / N Engl J Med 350(21) : 2167~2179

[journal] Moreland LW / 1997 / Treatment of rheumatoid arthritis with a recombinant human tumor necrosis factor receptor(p75)-Fc fusion protein / N Engl J Med 337(3) : 141~147

[journal] Den Broeder A / 2002 / A single dose, placebo controlled study of the fully human anti-tumor necrosis factor-α antibody adalimumab(D2E7)in patients with rheumatoid arthritis / J Rheumatol 29(11) : 2288~2298

[journal] Fleischmann RM / 2003 / Anakinra : A recombinant human interleukin-1 receptor antagonist(rmetHulL-1ra), in patients with rheumatoid arthritis : A large, international, multicenter, placebo-controlled trial / Arthritis Rheum 48(4) : 927~934

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