Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6
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pISSN : 1229-1854
- https://journal.kci.go.kr/jkmr
KJCKorea
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Study of Mori Fructus and Dried Mori Fructus Extracts on the Antioxidant Effect and the Inhibitory Effect on Adipocyte Differentiation
- Journal of Korean Medicine Rehabilitation
- Abbr : JKMR
- 2014, 24(4), pp.1-13
- Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
- Research Area : Medicine and Pharmacy > Korean Medicine
김형구
1,
Jing-Hua Wang
2,
Lim Dongwoo
3,
hee sung chae
4,
CHIN, YOUNG-WON
5,
Choi, Han-Seok
4,
Hojun Kim
4
1동국대학교 한의과대학 한방재활의학과교실
2동국대학교(경주캠퍼스) 한의학연구소
3동국대학교 한의과대학 한방병리학교실
4동국대학교
5동국대학교 약학대학 약학교실
Accredited
ABSTRACT
Objectives This study was to investigate the antioxidative capacity, antiobesity effect and anti-diabetes effects of Mori Fructus and dried Mori Fructus in Raw 264.7 cells and 3T3-L1 cells.
Methods 3 different types of Mori Fructus extracts (water 100%, ethanol 30%, ethanol 100%) were used in this study. And 3 different types of dried Mori Fructus extracts (water 100%, ethanol 30%, ethanol 100%) were used in this study. Total polyphenol compund, total favonoid compound, DPPH radical scavenging, ROS activity, NO, cell proliferation were measured in the experiment. Expressions of adipogenic transcription factors including C/EBP-α, C/EBP-β, PPAR-α, PPAR-γ, AMPK-α were analyzed by Real time PCR.
Results Mori Fructus extracts measurements are higher than dried Mori Fructus extracts measurements at Total flavonoid compound and total flavonoid compound. Mori Fructus extracts measurements are lower than dried Mori Fructus extracts measurements at DPPH radical scavenging, ROS activity, NO. In RT-PCR analysis, there is a tendency that dried Mori Fructus extracts inhibit the expression of C/EBP-α, C/EBP-β genes. In RT-PCR analysis, there is a tendency that dried Mori Fructus extracts promote the expression of PPAR-α, PPAR-γ, AMPK-α genes.
Conclusions Mori Fructus is effective on inhibiting the oxidation and dried Mori Fructus is effective on inhibiting the obesity and diabetes.
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[journal]
Spiegelman BM
/ 2001
/ Obesity and the regulation of energy balance
/ Cell
104
: 531~543
[journal]
유순집
/ 2008
/
Pharmacological Treatment of Obesity
/ Endocrinology and Metabolism
/ 대한내분비학회
23(4)
: 223~233
[web]
KFDA
/ 2013
/ Suspension of sale on Sibutramine
/ http://www.kfda.go.kr/antidrug/index.do?nMenuCode=53&mode=view&boardSeq=2367
[book]
Halliwell B
/ 1999
/ Free radicals in Biology and Medicine
/ Oxford University Press
: 1~321
[journal]
Chung HY
/ 1992
/ Aging and Carcinogenic Mechanisms Induced by Free Fadicals
/ Kor J Gerontol
2
: 1~11
[journal]
Virag L
/ 2003
/ Peroxynitrite induced cytotoxicity: mechanism and opportunities for intervention
/ Toxicol Lett
140(1)
: 113~124
[journal]
Balavoine GGA
/ 1999
/ Peroxynitrite scavenging by different antioxidants. Part 1: convenient assay
/ Nitric Oxide
3(1)
: 40~54
[journal]
Patel RP
/ 1999
/ Biological aspects of reactive nitrogen species
/ Biochim Biophys Acta
1411(2-3)
: 385~400
[book]
Harman D
/ 1986
/ Free radical theory of aging ; Role of free radicals in the organization and evolution of life, aging and disease processes. Free Radicals, Aging and Degenerative Disease
/ alan R. Liss.Inc
: 3~49
[journal]
張文彭
/ 1989
/ 淸宮長春丹對老年腎虛證血漿過氧化脂質高密度脂蛋白膽固醇水平影響的硏究
/ 中醫雜誌
30(3)
: 34~38
[journal]
Batteli NG
/ 1973
/ Milk xanthine oxidase type D(dehydrogenase) and type O(oxydase) :Purification and interconversion and some properties
/ Biochem. J
131
: 191~198
[journal]
Simon RH
/ 1981
/ Hydrogen peroxide cause the fatal injury to human fibroblast exposed to the oxygen radicals
/ J. Biol. Chem
266
: 7181~7186
[book]
李尙仁
/ 1981
/ 本草學
/ 醫藥社
: 135~
[book]
두호경
/ 1993
/ 東醫腎系學
/ 동양의학연구원
: 10~1329
[book]
王其飛
/ 1989
/ 中醫長壽學
/ 遼寧科學技術出版社
: 340~342
[journal]
Konno K
/ 2006
/ Mulberry latex rich in antidiabetic sugar-mimic alkaloids forces dieting on caterpillars. roc
/ Natl. Acad. Sci
103
: 1337~1341
[journal]
Chung KO
/ 2003
/ In-vitro and in-vivo anti-inflammatory effect of oxyresveratrol from Morus alba L
/ Pharmacol
55
: 1695~1700
[journal]
Zou, T. B.
/ 2011
/ Optimization of ultrasound-assisted extraction of anthocyanins from mulberry, using response surface methodology
/ Int. J. Mol. Sci
12
: 3006~3017
[journal]
Chen PN
/ 2006
/ Mulberry anthocyanins, cyanidin 3-rutinoside and cyanidin 3-glucoside, exhibited an inhibitory effect on the migration and invasion of a human lung cancer cell line
/ Cancer Lett
235
: 248~259
[journal]
Kim HB
/ 2003
/ Identification of C3G(cyanidin-3-glucoside) from mulberry fructus and quantification with defferent varieties
/ Korean J. seric. Sci
45
: 90~95
[journal]
Abd-El-Mawla AM
/ 2011
/ Induction of biologically active flavonoids in cell cultures of Morus nigra and testing their hypoglycemic efficacy
/ Sci. Pharm
79
: 951~961
[journal]
Kobayashi Y
/ 2010
/ Ameliorative effects of mulberry (Morus alba L.)leaves on hyperlipidemia in rats fed a high-fat diet: induction of fatty acid oxidation, inhibition of lipogenesis, and suppression of oxidative stress
/ Biosci. Biotechnol. Biochem
74
: 2385~2395
[journal]
Kim HB
/ 2004
/ Varietal analysis and quantification of amino acid in mulberry fructus
/ Korean J. Seric. Scl
46
: 47~53
[journal]
Imran M
/ 2011
/ Chemical composition and antioxidant activity of certain Morus species
/ J. Zhejiang Univ. Sci. B
11
: 973~980
[journal]
Jin YS
/ 2007
/ Identification and properties of 2,5-dihydroxy-4,3'-di(beta-D-glucopyranosyloxy)-transstilbene from Morus bombycis Koidzumi roots
/ Phytother. Res
21
: 605~608
[journal]
Bae SH
/ 2007
/ Antioxidant activities of five different mulberry cultivars in Korea
/ Food Sci. and Tech
40
: 955~962
[journal]
Kim SY
/ 1998
/ Antiimflammatory and antioxidative effects of Morus spp. fructus extract
/ Korean J. Med. Corp. Sci
6
: 204~209
[journal]
Kimura T
/ 2007
/ Food-grade mulberry powder enriched with 1-deoxynojirimycin suppresses the elevation of post prandial blood glucose in humans
/ J. Agric. Food Chem
55
: 5869~5874
[journal]
Park MY
/ 2005
/ Effects of dietary mulberry, Korean red ginseng, and banaba on glucose homeostasis in relation to PPAR-alpha, PPAR-gamma, and LPL mRNA expressions
/ Life Sci
77
: 3344~3354
[journal]
Kim HB
/ 2001
/ Effect of methanol extracts from mulberry fructus on the lipid metalbolism and liver function in cholesterolinduced hyperlipidemia rats
/ Korean J. Seri. Sci
42
: 104~108
[book]
두호경
/ 1993
/ 동의신계학
/ 동양의학연구원
: 10~1329
[book]
Harman D.
/ 1986
/ Free radical theory of aging ; Role of free radicals in the organization and evolution of life, aging and disease processes. Free Radicals, Aging and Degenerative Disease
/ alan R. Liss.Inc
: 3~49
[journal]
張文彭
/ 1989
/ 淸宮長春丹對老年腎虛證血漿過.化脂質高密度脂蛋白膽固醇水平影響的硏究
/ 中醫雜誌
30(3)
: 34~38
[journal]
Batteli NG
/ 1973
/ Milk xanthine oxidase type D(dehydrogenase) and type O(oxydase) :Purification and interconversion and some properties
/ Biochem. J
131
: 191~198
[journal]
Simon RH
/ 1981
/ Hydrogen peroxide cause the fatal injury to human fibroblast exposed to the oxygen radicals
/ J. Biol. Chem
266
: 7181~7186
[journal]
이지영
/ 2004
/
Influence of the UCP2 Gene Polymorphism on Obesity Indices, Cardiorespiratory Fitness, and Obesity-related Metabolic Syndrome Markers in Non-obese and Obese Subjects
/ Journal of Exercise Nutrition & Biochemistry
/ 한국운동영양학회
8(3)
: 347~354
[journal]
안원근
/ 2003
/ 노화과정에 있어서 P21 유전자의 역할
/ 생화학뉴스
23(2)
: 80~81
[journal]
여에스더
/ 2002
/ 폐경기 노화와 비만
/ 대한비만학회지
11(3)
: 294~
[book]
王其飛
/ 1989
/ 中醫長壽學
/ 遼寧科學技術出版社
: 340~342
[journal]
장보윤
/ 2012
/
Acute Toxicity Study on the Extract of Mori Fructus
/ 생약학회지
/ 한국생약학회
43(2)
: 179~183
[journal]
박용기
/ 1999
/ 상심자의 항산화 작용에 관한 연구
/ 대한본초학회지
14(2)
: 43~50
[thesis]
박병철
/ 2007
/ 상심자의 항산화 효과 및 3T3-L1 세포주의 유전자 발현과 cytokine에 미치는 영향
/ 박사
/ 상지대학교 대학원
[journal]
이병규
/ 1998
/ 비만의 개념 및 변증시치에 관한 문헌적고찰
/ 대전대학교 한의학연구소 논문집
7(1)
: 533~541
[journal]
안형수
/ 2001
/
Effects of Water Extract of Several Herbs on Change of
Weight and Serum of Obesity Rats Induced by High Fat Diet
/ 동의생리병리학회지
/ 한의병리학회
15(4)
: 537~542
[journal]
Rosen ED
/ 2000
/ Transcriptional regulation of adipogenesis
/ Genes Dev
14
: 1293~1307
[journal]
Jeon T
/ 2004
/ Red yeast rice extracts suppress adipogenesis by down-regulating adipogenic transcription factors and gene expression in 3T3-L1 cells
/ Life Sci
75
: 3195~3203
[journal]
Cowherd RM
/ 1999
/ Molecular regulation of adipocyte differentiation
/ Semin Cell Dev Biol
10
: 3~10
[journal]
Cornelius P
/ 1994
/ Regulation of adipocyte development
/ Annu Rev Nutr
14
: 99~129
[journal]
Morrison RF
/ 2000
/ Hormonal signaling and transcriptional control of adipocyte differentiation
/ J Nutr
130
: 3116S~3121S
[journal]
Rosen ED
/ 2006
/ Adipocyte differentiation from the inside out
/ Nat Rev Mol Cell Biol
7
: 885~896
[journal]
Auboeuf D
/ 1997
/ Tissue distribution and quantification of the expression of mRNAs of peroxisome proliferator-activated receptors and liver X receptor-alpha in humans: no alteration in adipose tissue of obese and NIDDM patients
/ Diabetes
46(8)
: 1319~1327
[journal]
Guo W
/ 2006
/ Fatty acid transport and metabolism in HepG2 cells
/ Am J Physiol Gastrointest Liver Physiol
290(3)
: 528~534
[journal]
Dreyer C
/ 1992
/ Control of the peroxisomal beta-oxidation pathway by a novel family of nuclear hormone receptors
/ Cell
68(5)
: 879~887
[journal]
Schoonjans K
/ 1996
/ PPARalpha and PPARgamma activators direct a distinct tissue-specific transcriptional response via a PPRE in the lipoprotein lipase gene
/ EMBO J
15(19)
: 5336~5348
[journal]
Vu-Dac N
/ 1998
/ The nuclear receptors peroxisome proliferator-activated receptor alpha and Rev-erbalpha mediate the species-specific regulation of apolipoprotein A-I expression by fibrates
/ J Biol Chem
273(40)
: 25713~25720
[journal]
Rossen ED
/ 1999
/ PPAR gamma is required for the differentiatio of adipose tissue in vivo and in vitro
/ Mol Cell
4
: 611~617
[journal]
이규호
/ 2008
/
Association Study of the Peroxisome Proliferators-Activated Receptor γ2 Pro12Ala Polymorphism with Diabetic Nephropathy
/ Diabetes and Metabolism Journal
/ 대한당뇨병학회
32(5)
: 402~408
[journal]
Rossen ED
/ 1999
/ PPAR gamma is required for the differentiatio of adipose tissue in vivo and in vitro
/ Mol Cell
4
: 611~617
[journal]
Nammi S
/ 2009
/ Protective effects of ethanolic extract of Zingiber officinale rhizome on the development of metabolic syndrome in high-fat diet-fed rats
/ Basic. Clin. Pharmacol. Toxicol
104
: 366~
[journal]
Kola B
/ 2008
/ The role of AMP-activated protein kinase in obesity
/ Front Horm. Res
36
: 198~
[journal]
Yano W
/ 2008
/ Molecular mechanism of moderate insulin resistance in adiponectin-knockout mice
/ Endocr. J
55
: 515~
[journal]
Janovska A
/ 2007
/ AMPK and ACC phosphorylation:Effect of leptin, muscle fibre type and obesity
/ Mol. Cell. Endocrinol
284
: 1~
[journal]
Pang J
/ 2008
/ Ilex paraguariensis extract ameliorates obesity induced by high-fat diet: potential role of AMPK in the visceral adipose tissue
/ Arch. Biochem. Biophys
476
: 178~
[journal]
Landree LE
/ 2004
/ C75, a fatty acid synthase inhibitor, modulates AMP-activated protein kinase to alter neuronal energy metabolism
/ J. Biol. Chem
279
: 3817~