Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6
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pISSN : 1229-1854
- https://journal.kci.go.kr/jkmr
KJCKorea
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Study of Mori Fructus and Dried Mori Fructus Extracts on the Antioxidant Effect and the Inhibitory Effect on Adipocyte Differentiation
- Journal of Korean Medicine Rehabilitation
- Abbr : JKMR
- 2014, 24(4), pp.1-13
- Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
- Research Area : Medicine and Pharmacy > Korean Medicine
김형구
1,
Jing-Hua Wang
2,
Lim Dongwoo
3,
hee sung chae
4,
CHIN, YOUNG-WON
5,
Choi, Han-Seok
4,
Hojun Kim
4
1동국대학교 한의과대학 한방재활의학과교실
2동국대학교(경주캠퍼스) 한의학연구소
3동국대학교 한의과대학 한방병리학교실
4동국대학교
5동국대학교 약학대학 약학교실
Accredited
ABSTRACT
Objectives This study was to investigate the antioxidative capacity, antiobesity effect and anti-diabetes effects of Mori Fructus and dried Mori Fructus in Raw 264.7 cells and 3T3-L1 cells.
Methods 3 different types of Mori Fructus extracts (water 100%, ethanol 30%, ethanol 100%) were used in this study. And 3 different types of dried Mori Fructus extracts (water 100%, ethanol 30%, ethanol 100%) were used in this study. Total polyphenol compund, total favonoid compound, DPPH radical scavenging, ROS activity, NO, cell proliferation were measured in the experiment. Expressions of adipogenic transcription factors including C/EBP-α, C/EBP-β, PPAR-α, PPAR-γ, AMPK-α were analyzed by Real time PCR.
Results Mori Fructus extracts measurements are higher than dried Mori Fructus extracts measurements at Total flavonoid compound and total flavonoid compound. Mori Fructus extracts measurements are lower than dried Mori Fructus extracts measurements at DPPH radical scavenging, ROS activity, NO. In RT-PCR analysis, there is a tendency that dried Mori Fructus extracts inhibit the expression of C/EBP-α, C/EBP-β genes. In RT-PCR analysis, there is a tendency that dried Mori Fructus extracts promote the expression of PPAR-α, PPAR-γ, AMPK-α genes.
Conclusions Mori Fructus is effective on inhibiting the oxidation and dried Mori Fructus is effective on inhibiting the obesity and diabetes.
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