@article{ART001957812},
author={조창영 and Lee In Hee and 이재웅 and 김은지 and 이진호 and 김민정},
title={The Effects of High Temperature High Pressure Steam Sterilization on Woohwangchungsimwon},
journal={Journal of Korean Medicine Rehabilitation},
issn={1229-1854},
year={2015},
volume={25},
number={1},
pages={45-52}
TY - JOUR
AU - 조창영
AU - Lee In Hee
AU - 이재웅
AU - 김은지
AU - 이진호
AU - 김민정
TI - The Effects of High Temperature High Pressure Steam Sterilization on Woohwangchungsimwon
JO - Journal of Korean Medicine Rehabilitation
PY - 2015
VL - 25
IS - 1
PB - The Korean Academy Of Oriental Rehabilitation Medicine
SP - 45
EP - 52
SN - 1229-1854
AB - ObjectivesTo check marker content for appropriate quality control of Woohwangchungsimwon sterilized to ensure microbiological safety and to observe antioxidant activity for any changes in efficacy.
MethodsTo measure any effects of sterilization on the effective compounds, 8 ingredients of Woohwangchungsimwon were screened for any changes in marker content using HPLC-DAD. Using the colorimetric method on the microplate reader any changes in total phenolic compound and flavonoid levels were observed. Antioxidant activity was measured using the DPPH, ABTS, and FRAP.
ResultsOf the ingredients of Woohwangchungsimwon, 8 were subject to quantitative analysis before and after sterilization. 21.6 mg and 1.93 mg of Glycyrrhizin was found in Glycyrrhiza uralensis Fischer pre and post sterilization, respectively. Decursin found in Angelica gigas Nakai increased from 0.16 mg to 0.29 mg after sterilization. Bilirubin found in Gallstone of Bostaurusvar. domesticus increased from 0.24 mg to 0.33 mg. Cinnamic acid found in Cinnamomum cassia Blume increased from 0.02 mg to 0.05 mg. Ginsenoside Rb1 found in Panax ginseng C. A. Meyer decreased from 0.02 mg to 0.14 mg. Paeoniflorin found in Paeonia lactiflora Pallas increased from 1.05 mg to 1.13 mg. Amygdalin found in Armeniacae Amarum Semen increased from 2.68 mg to 2.83 mg. L-muscone found in Musk increased from 0.63 mg to 0.76 mg. As for total phenolic compound and total flavonoid content, there was a 1.22 and 4.15-fold increase. DPPH and ABTS increased by 20.45% and 20.69%, respectively. FRAP activity was 2.78 times more active post stabilization.
ConclusionsThis study confirmed that high temperature high pressure steam sterilization, a method used to ensure microbiological safety of Woohwangchungsimwon, does not affect marker content; in other words, does not affect quality of the Woohwangchungsimwon. It could also be seen that total phenolic compound and flavonoid content increased after sterilization. An antioxidant activity test showed that there was significantly increased activity of antioxidants. (J Korean Med Rehab 2015;25(1):45-52)
KW - Woohwangchungsimwon;HPLC;DPPH;ABTS;FRAP;Sterilization
DO -
UR -
ER -
조창영, Lee In Hee, 이재웅, 김은지, 이진호 and 김민정. (2015). The Effects of High Temperature High Pressure Steam Sterilization on Woohwangchungsimwon. Journal of Korean Medicine Rehabilitation, 25(1), 45-52.
조창영, Lee In Hee, 이재웅, 김은지, 이진호 and 김민정. 2015, "The Effects of High Temperature High Pressure Steam Sterilization on Woohwangchungsimwon", Journal of Korean Medicine Rehabilitation, vol.25, no.1 pp.45-52.
조창영, Lee In Hee, 이재웅, 김은지, 이진호, 김민정 "The Effects of High Temperature High Pressure Steam Sterilization on Woohwangchungsimwon" Journal of Korean Medicine Rehabilitation 25.1 pp.45-52 (2015) : 45.
조창영, Lee In Hee, 이재웅, 김은지, 이진호, 김민정. The Effects of High Temperature High Pressure Steam Sterilization on Woohwangchungsimwon. 2015; 25(1), 45-52.
조창영, Lee In Hee, 이재웅, 김은지, 이진호 and 김민정. "The Effects of High Temperature High Pressure Steam Sterilization on Woohwangchungsimwon" Journal of Korean Medicine Rehabilitation 25, no.1 (2015) : 45-52.
조창영; Lee In Hee; 이재웅; 김은지; 이진호; 김민정. The Effects of High Temperature High Pressure Steam Sterilization on Woohwangchungsimwon. Journal of Korean Medicine Rehabilitation, 25(1), 45-52.
조창영; Lee In Hee; 이재웅; 김은지; 이진호; 김민정. The Effects of High Temperature High Pressure Steam Sterilization on Woohwangchungsimwon. Journal of Korean Medicine Rehabilitation. 2015; 25(1) 45-52.
조창영, Lee In Hee, 이재웅, 김은지, 이진호, 김민정. The Effects of High Temperature High Pressure Steam Sterilization on Woohwangchungsimwon. 2015; 25(1), 45-52.
조창영, Lee In Hee, 이재웅, 김은지, 이진호 and 김민정. "The Effects of High Temperature High Pressure Steam Sterilization on Woohwangchungsimwon" Journal of Korean Medicine Rehabilitation 25, no.1 (2015) : 45-52.