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Identification of Novel Standard Compounds for Standardization of Sinbaro3 Pharmacopuncture

  • Journal of Korean Medicine Rehabilitation
  • Abbr : JKMR
  • 2015, 25(2), pp.65-72
  • Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine

Lee In Hee 1 이진호 1 이재웅 1 김민정 1 김은지 1

1자생의료재단

Accredited

ABSTRACT

ObjectivesTo investigate and validate potential standard compounds for standardization of Sinbaro3 pharmacopuncture prepared at OO Hospital of Korean Medicine.MethodsSinbaro3 pharmacopuncture was prepared by extraction, purification and hydrolysis of Harpagophytum procumbens, and various potential standard compounds were quantified through HPLC-UV and HPLC-MS analysis. Validation was examined by assessing specificity, linearity, precision, and accuracy.ResultsThe retention time of harpagide and cinnamic acid were 15.2 min and 28.2 min, respectively, and both showed good linearity in analysis by concentration at 0.9999 and 0.9998, respectively. Intra-day variation of precision was 0.0015~0.0045% and 0.0058~ 0.1629%, while inter-day variation of precision was 0.0011~0.0243% and 0.0098~0.1629%,and that of accuracy was 99.53~99.89% and 99.50~99.91%, respectively.ConclusionsHarpagide and cinnamic acid, which are hydrolyzates of harpagoside within Sinbaro3 pharmacopuncture, were both validated using HPLC-MS and HPLC-UV analysis, and Sinbaro3 pharmacopuncture contained 78.41 ug/ml harpagide, and 2.05 ug/ml cinnamic acid. (J Korean Med Rehab 2015;25(2):65-72)

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