2015, Vol.25, No.2

Antibacterial Effect of Haedokgeumhwa-san against Methicillin-Resistant Staphylococcus aureus

SONG YUNG SUN , 이하일 , LEE SU KYUNG and 1 other persons | 2015, 25(2) | pp.1~13 | number of Cited : 1

ObjectivesMethicillin-Resistant Staphylococcus aureus (MRSA) is a human pathogen and a major cause of hospital-acquired infections. New antibacterial agents that have not been compromised by bacterial resistance are needed to treat MRSA-related infections. In this study, we investigated the antimicrobial activity ofethanol extract of Haedokgeumhwa-san (HGH) which prescription is composed of korean medicine against MRSA. MethodsThe antibacterial activity of HGH extract was evaluated against MRSA strains by using the Disc diffusion method, broth microdilution method (minimal inhibitory concentration; MIC), checkerboard dilution test, and time-kill test; its mechanism of action was investigated by bacteriolysis, detergent or ATPase inhibitors. The checkerboard dilution test was used to examined synergistic effect of ampicillin, oxacillin, ciprofloxacin, vancomycin, gentamicin and norfloxacin in combination with HGH ethanol extract. A time-kill assay was performed a survival curve which was obtained by plotting viable colony counts depending on time on bacterial growth. ResultsThe minimum inhibitory concentration (MIC) of ethanol extract (HGH) ranged from 1,000 to 2,000 μg/mL against all the tested bacterial strains, respectively. We are able to confirm that HGH extract has potentially strong antibacterial activity. In the checkerboard dilution test, fractional inhibitory concentration index of HGH in combination with antibiotics indicated synergy or partial synergism against S. aureus. A time-kill study showed that the growth of the tested bacteria was considerably inhibited after 8 hr of treatment with the combination of HGH with selected antibiotics. For measurement of cell membrane permeability, HGH 250~1,000 μg/mL along with concentration of Triton X-100 (TX) and Tris-(hydroxymethyl) aminomethane (Tris) were used. In the other hand, N,N-dicyclohexylcarbodimide (DCCD) and Sodium azide (NaN3) was used as an inhibitor of ATPase. TX, Tris, DCCD and NaN3 cooperation against S. aureus showed synergistic action. Accordingly, antimicrobial activity of HGH was affected by cell membrane and inhibitor of ATPase. ConclusionsThese results suggest that Haedokgeumhwa-san extract has antibacterial activity, and that HGH extract offers a potential as a natural antibiotic against MRSA. (J Korean Med Rehab 2015;25(2):1-13)

Effects of Bujasasim-tang Ethanol Extract on Oxidative Stress, Inflammation and Osteoarthritic Rat Model

Minseok Oh , Chang-Hoon Woo | 2015, 25(2) | pp.15~35 | number of Cited : 4

ObjectivesThis study was performed to investigate the effects of Bujasasim-tang ethanol extract (BST) on oxidative stress, inflammation and osteoarthritic rat model.MethodsTo ensure safety of BST, heavy metal levels were measured and cytotoxicity test was done. In vitro, To evaluate antioxidative effects of BST, total phenolic contents, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2’–azino–bis-(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) scavenging activity, reactive oxygen species (ROS) levels were measured. Also, to evaluate anti-inflammatory effects of BST treated group, total nitric oxide (NO) and pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) levels were measured in lipopolysaccharide(LPS)-stimulated RAW 264.7 cells. In vivo, We injected MIA 50 μl (60 mg/ml) into knee joints of rats to induce osteoarthritis. Rats were divided into total 3 groups (normal, control, BST treated group, each n=7). Normal group was not treated at all without inducing osteoarthritis and taken normal diet. Control group was induced osteoarthritis by MIA and taken with 2 ml of distilled water once a day for 4 weeks. BST treated group was induced osteoarthritis by MIA and taken BST 2 ml (200 mg/kg/mouse) once a day for 4 weeks. We evaluated dynamic weight bearing with the Incapacitance Test Meter. At the end of experiment, the rats were sacrificed to observe the functions of liver and kidney, changes of WBC, neutrophil, lymphocyte, monocyte levels in blood, to evaluate the levels of pro-inflammatory cytokines, tissue inhibitor of metallopreteinases-1 (TIMP-1), matrix metalloproteinase-9 (MMP-9), prostaglandin E2 (PGE2), leukotriene B4 (LTB4) within serum. We observed change of articular structures by Hematoxylin & Eosin (H&E), safranin-O staining method and measured amount of cartilage by micro CT-arthrography. Statistical analysis was done by unpaired student’s t-test with significance level at p＜0.05 in SPSS 11.0 for windows.Results1. Safety of the BST was identified. 2. AST, ALT, BUN, creatinine levels of BST treated group were within normal limit. In vitro, 1. DPPH and ABTS free radical scavenging activities of BST showed dose-dependent increase. 2. ROS production were significantly decreased. 3. Total nitric oxide (NO) and IL-1β production were decreased. 4. IL-6 and TNF-α production were significantly decreased. In vivo, 1. Weight bearing ability was significantly increased. 2. WBC, neutrophil, lymphocyte, monocyte levels in blood were decreased. 3. IL-1β and TNF-α levels in serum were significantly decreased. and the IL-6 level was decreased. 4. TIMP-1, MMP-9, LTB4, PGE2 levels in serum were significantly decreased. 5. Cartilage volume of BST treated group was significantly increased. Also changes of cartilage, synovial membrane, fibrous tissue were suppressed.ConclusionsThe results obtained in this study Bujasasim-tang have effects of anti-oxidative, antiinflammatory, relieve pain and protection of cartilage. Therefore we expect that Bujasasim-tang is effective treatment for osteoarthritis. (J Korean Med Rehab 2015;25(2):15-35)

Effects of Danggwisayeok-tang (Dangguisinitang) on MIA-Induced Osteoarthritis Rats

Hee Duk An , Yang Doo Hwa , Chang-Hoon Woo and 1 other persons | 2015, 25(2) | pp.37~50 | number of Cited : 5

ObjectivesThe purpose of this study was to know the effects of Danggwisayeok-tang (Dangguisinitang) extract (DGSYT) on monosodium iodoacetate (MIA)-induced rat osteoarthritis.MethodsFor this purpose, rats were divided into 5 groups. Normal group was not injected with MIA and orally administered any medication. Control group was injected with MIA and not orally administered any medication. DGSYT100 group was injected with MIA and orally administered 100 mg/kg of DGSYT. DGSYT300 group was injected with MIA and orally administered 300 mg/kg of DGSYT. JoinsT group was injected with MIA and orally administered 20 mg/kg of Joins tablet. DGSYT100 and DGSYT300 groups were orally administered DGSYT during a week before and 3 weeks after based on the day MIA injected. The changes of hepatotoxicity, nephrotoxicity, relative hind paw weight distribution, cytokine in serum, cytokine messenger ribonucleic acid (mRNA) in joint tissue and histopathological observation (Hematoxylin & Eosin and Safranin-O staining) were measured.ResultsAlanine aminotransferase (ALT) levels of DGSYT100, DGSYT300 and JoinsT groups were increased significantly, but these results were within normal range. Aspartate aminotransferase (AST) and creatinine levels of all groups were not changed significantly. In the change of relative hind paw weight distribution, DGSYT300 and JoinsT groups were decreased significantly 14 and 21 days after MIA injected. Interleukin-1β (IL-1β) and Interleukin-6 (IL-6), Leukotriene B4 and Osteocalcin levels of DGSYT300 and JoinsT groups were decreased significantly. In measurement of IL-1β and nitric oxide synthase-Ⅱ mRNA relative quantitative of control, DGSYT100, DGSYT300 and JoinsT groups were decreased significantly. In measurement of TNF-α, IL-6 and Cyclooxygenase-2 mRNA relative quantitative of control, DGSYT300 and JoinsT groups was decreased significantly. In histopathological observation of knee, synovial tissue, cartilage and proteoglycan of DGSYT100, DGSYT300 and JoinsT were well preserved compared with control group.ConclusionsAccording to the results, DGSYT has anti-inflammation and pain relief effects. So it should be suppressed progression of arthritis in MIA-induced osteoarthritis rat. (J Korean Med Rehab 2015;25(2):37-50)

Effects of Gyejigabuja-tang on MIA-induced Osteoarthritis in Rats

Chang-Hoon Woo , 원제훈 | 2015, 25(2) | pp.51~64 | number of Cited : 6

Identification of Novel Standard Compounds for Standardization of Sinbaro3 Pharmacopuncture

Lee In Hee , 이진호 , 이재웅 and 2 other persons | 2015, 25(2) | pp.65~72 | number of Cited : 3

Single-dose Intramuscular Toxicity Studies of Shinbaro3 Pharmacopunture in Sprague-Dawley Rats and Beagle Dogs

김민정 , 이진호 , Lee In Hee and 2 other persons | 2015, 25(2) | pp.73~80 | number of Cited : 4

Analysis of the Research Trends of Pelvic Malposition in Korean Clinical Research

강준혁 , 박재현 , 남수현 and 2 other persons | 2015, 25(2) | pp.81~88 | number of Cited : 2

Trends of Chuna Manual Therapy on Traffic Accident in Korea from 2005 to 2014

Ko YounSeok , 전용태 , 박상훈 and 2 other persons | 2015, 25(2) | pp.89~96 | number of Cited : 9

Acupuncture Treatment of Plantar Fasciitis: A Literature Review

Lee Jung-han , 고낙용 , 김창곤 and 1 other persons | 2015, 25(2) | pp.97~110 | number of Cited : 9

A Study of Bi-Jeung in the Mid-Chosun Dynasty: Based on the Seungjeongwon Ilgi

Chung, Seok Hee , 조우영 , 정재영 | 2015, 25(2) | pp.111~118 | number of Cited : 0

The Retrospective Medical Charts Review of 459 Neck Pain Inpatients in Korean Medicine Hospital

Minseok Oh , 허건 | 2015, 25(2) | pp.119~134 | number of Cited : 1

Characteristics of 601 Low Back Pain Patients: A Korean Medicine Hospital Experience, Retrospective Chart Review

Minseok Oh , 남대진 | 2015, 25(2) | pp.135~153 | number of Cited : 4

ObjectivesThe purpose of this study was to investigate the characteristics of inpatients who were admitted to Korean medicine hospital due to low back pain.MethodsThe current study was designed as a retrospective chart review to investigate descriptive characteristics of LBP patients. The clinical records of 601 patients who were hospitalized for treatment of LBP in Cheonan Korean Medicine Hospital, Daejeon University from 1st, January, 2013 to 31th, December, 2013 were analyzed.Results1. Most frequently given diagnosis was sprain and strain of lumbar (45.93%). 2. Female outnumbered male patients in all disease groups except fracture of lumbar spine. 3. In distribution according to age, sprain and strain of lumbar, HNP of L-spine and lumbago with sciatica were most frequent at 30s, fracture of lumbar spine was most frequent at 50s and spinal stenosis was most frequent at 70s. 4. The most frequently motive for low back pain was traffic accident (35.4%) 5. Patients with no related medical history were 76.95% 6. 0~1week interval between onset and visit to Korean Medicine Hospital was most frequent in all disease groups. 7. 50.85% of patients went through treatment at medical institutions before the admitting to Korean Medicine Hospital. 8. The average admission days of female was higher than male’s. And age goes up, average duration of admission was longer. 9. In most (74.59%) of the patients, symptoms were more than improved. 10. Most frequently prescribed examination was X-ray (65.13%). 11. Most frequently prescribed herb medicine was whal-lak-tang (Huoluo-tang).ConclusionsIn most (74.59%) of the patients, symptoms were more than improved, especially in sprain and strain of lumbar and lumbago with sciatica. But Patients with a local hospital statistic is not be representative of the incidence of the population. In order to obtain more accurate statistics, it is necessary to compare analysis collect statistics from other medical hospitals. (J Korean Med Rehab 2015;25(2):135-153)

The Retrospective Review of 410 Shoulder Pain Inpatients in Korean Medicine Hospital

Minseok Oh , 이재은 | 2015, 25(2) | pp.155~173 | number of Cited : 4

A Case Report of Neck Pain Patient with Klippel-Feil Syndrome by Cervical Manipulation Treatment

금동호 , 이종하 , 권정국 | 2015, 25(2) | pp.175~180 | number of Cited : 0

A Clinical Case Study of Operation-delayed Cauda Equina Syndrome Patient Improved by Korean Traditional Treatment

Suhyeon Jeong , 정해창 | 2015, 25(2) | pp.181~187 | number of Cited : 2

The Clinical Study of Oriental Medicine on Spontaneous Osteonecrosis of the Knee

이상욱 , 양재철 , 옥소윤 and 2 other persons | 2015, 25(2) | pp.189~196 | number of Cited : 0

Deep Vein Thrombosis after Total Knee Replacement in a Patient undergoing Korean Medical Rehabilitation

Lee Jung-han , 김창곤 , 고낙용 and 1 other persons | 2015, 25(2) | pp.197~203 | number of Cited : 3

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Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6

2015, Vol.25, No.2